Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Monogalactosyldiacylglycerol synthase, chloroplastic (SoMGD1) (EC 2.4.1.46) (MGDG synthase type A)

 MGDG_SPIOL              Reviewed;         522 AA.
Q9SM44;
02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
01-MAY-2000, sequence version 1.
28-MAR-2018, entry version 46.
RecName: Full=Monogalactosyldiacylglycerol synthase, chloroplastic;
Short=SoMGD1;
EC=2.4.1.46;
AltName: Full=MGDG synthase type A;
Flags: Precursor;
Name=MGD A;
Spinacia oleracea (Spinach).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; Caryophyllales; Chenopodiaceae; Chenopodioideae;
Anserineae; Spinacia.
NCBI_TaxID=3562;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, SUBCELLULAR
LOCATION, AND SUBUNIT.
PubMed=10518794; DOI=10.1046/j.1432-1327.1999.00801.x;
Miege C., Marechal E., Shimojima M., Awai K., Block M.A., Ohta H.,
Takamiya K., Douce R., Joyard J.;
"Biochemical and topological properties of type A MGDG synthase, a
spinach chloroplast envelope enzyme catalyzing the synthesis of both
prokaryotic and eukaryotic MGDG.";
Eur. J. Biochem. 265:990-1001(1999).
[2]
CHARACTERIZATION.
PubMed=8119920;
Marechal E., Block M.A., Joyard J., Douce R.;
"Kinetic properties of monogalactosyldiacylglycerol synthase from
spinach chloroplast envelope membranes.";
J. Biol. Chem. 269:5788-5798(1994).
[3]
CHARACTERIZATION, AND ENZYME REGULATION.
PubMed=7890698; DOI=10.1074/jbc.270.11.5714;
Marechal E., Miege C., Block M.A., Douce R., Joyard J.;
"The catalytic site of monogalactosyldiacylglycerol synthase from
spinach chloroplast envelope membranes. Biochemical analysis of the
structure and of the metal content.";
J. Biol. Chem. 270:5714-5722(1995).
[4]
BIOPHYSICOCHEMICAL PROPERTIES, 3D-STRUCTURE MODELING, AND MUTAGENESIS
OF TRP-171; GLU-173; TRP-177; HIS-240; HIS-245; CYS-284; CYS-286;
343-GLY--GLY-347; GLU-346; CYS-378; ARG-380; PHE-402; CYS-415;
LYS-419; GLU-427; GLU-445 AND ASN-448.
PubMed=16009708; DOI=10.1074/jbc.M505622200;
Botte C., Jeanneau C., Snajdrova L., Bastien O., Imberty A.,
Breton C., Marechal E.;
"Molecular modeling and site-directed mutagenesis of plant chloroplast
monogalactosyldiacylglycerol synthase reveal critical residues for
activity.";
J. Biol. Chem. 280:34691-34701(2005).
-!- FUNCTION: Involved in the synthesis of the major structural
component of photosynthetic membranes. The 1,2-diacylglycerol
substrate preference is 18:2/18:2 > 18:0/18:1 > 18:1/18:1 >
18:1/16:0 > 16:0/18:2 > 18:3/18:3 > 16:0/18:1 > 16:0/16:0 >
18:0/18:0. {ECO:0000269|PubMed:10518794}.
-!- CATALYTIC ACTIVITY: UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
= UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.
-!- COFACTOR:
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
-!- ENZYME REGULATION: Inhibited by ortho-phenanthroline and UDP
(competitive inhibitor relatively to UDP-Gal only) and inactivated
by citraconic anhydride, tert-butoxycarbonyl-L-methionine
hydrosuccinimidyl ester (SLR) and N-ethylmaleimide (NEM).
{ECO:0000269|PubMed:10518794, ECO:0000269|PubMed:7890698}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=0.65 mM for UDP-Gal {ECO:0000269|PubMed:16009708};
-!- SUBUNIT: Homodimer. {ECO:0000305}.
-!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
{ECO:0000269|PubMed:10518794}.
-!- DOMAIN: The C-terminal domain (308-483) is involved in UDP-Gal
binding while the N-terminal domains (131-307) is involved in
dyacylglycerol binding.
-!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
{ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AJ249607; CAB56218.1; -; mRNA.
ProteinModelPortal; Q9SM44; -.
SMR; Q9SM44; -.
CAZy; GT28; Glycosyltransferase Family 28.
KEGG; ag:CAB56218; -.
KO; K03715; -.
BRENDA; 2.4.1.46; 5812.
SABIO-RK; Q9SM44; -.
GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
GO; GO:0046509; F:1,2-diacylglycerol 3-beta-galactosyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
InterPro; IPR009695; Diacylglyc_glucosyltr_N.
InterPro; IPR007235; Glyco_trans_28_C.
Pfam; PF04101; Glyco_tran_28_C; 1.
Pfam; PF06925; MGDG_synth; 1.
1: Evidence at protein level;
Chloroplast; Glycosyltransferase; Membrane; Plastid;
Plastid inner membrane; Transferase; Transit peptide.
TRANSIT 1 98 Chloroplast. {ECO:0000255}.
CHAIN 99 522 Monogalactosyldiacylglycerol synthase,
chloroplastic.
/FTId=PRO_5000065674.
SITE 422 422 Substrate specificity for galactose.
MUTAGEN 171 171 W->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 173 173 E->N: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 177 177 W->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 240 240 H->A: Total loss of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 245 245 H->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 284 284 C->A: No effect.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 286 286 C->A: No effect.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 343 347 Missing: Total loss of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 346 346 E->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 378 378 C->A: 25% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 380 380 R->E: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 402 402 F->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 415 415 C->A: No effect.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 419 419 K->A: Total loss of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 427 427 E->A: Total loss of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 445 445 E->A: Total loss of activity.
{ECO:0000269|PubMed:16009708}.
MUTAGEN 448 448 N->A: 90% reduction of activity.
{ECO:0000269|PubMed:16009708}.
SEQUENCE 522 AA; 57511 MW; 02E2B929732551A7 CRC64;
MSHPSTVTSE PSNLLDFVPK LGNFVLNSSL HGNNSNGYSS FSSNSVHFGG LATQNRYKFV
NSLSFSKEGS NLKRILSDFN RVIRLHCDRI PLGFSSIGLN SGESNGVSDN GHGVLEDVRV
PVNAVEPESP KRVLILMSDT GGGHRASAEA IKAAFNEEFG DDYQVFVTDL WSEHTPWPFN
QLPRSYNFLV KHGPLWKMMY YGTSPRVIHQ SNFAATSVFI AREVARGLMK YQPDIIISVH
PLMQHVPLRI LRGRGLLEKI VFTTVVTDLS TCHPTWFHKL VTRCYCPSNE VAKRATKAGL
QPSQIKVYGL PVRPSFVRSV RPKNELRKEL GMDEHLPAVL LMGGGEGMGP IEATARALGN
ALYDANLGEP TGQLLVICGR NKKLAGKLSS IDWKIPVQVK GFVTKIEECM GACDCIITKA
GPGTIAEAMI RGLPIILNDY IAGQEAGNVP YVIENGIGKY LKSPKEIAKT VSQWFGPKAN
ELQIMSQNAL KHARPDAVFK IVHDLDELVR QKIFVRQYSC AA


Related products :

Catalog number Product name Quantity
E0167h ELISA Beta-trace protein,Cerebrin-28,Glutathione-independent PGD synthase,Homo sapiens,Human,Lipocalin-type prostaglandin-D synthase,PDS,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandi 96T
E0167h ELISA kit Beta-trace protein,Cerebrin-28,Glutathione-independent PGD synthase,Homo sapiens,Human,Lipocalin-type prostaglandin-D synthase,PDS,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostag 96T
E0167r ELISA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds,Rat,Rattus norvegicus 96T
U0167b CLIA Bos taurus,Bovine,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS 96T
E0167m ELISA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,L-PGDS,Mouse,Mus musculus,PGD2 synthase,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds 96T
U0167m CLIA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,L-PGDS,Mouse,Mus musculus,PGD2 synthase,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds 96T
U0167r CLIA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds,Rat,Rattus norvegicus 96T
E0167m ELISA kit Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,L-PGDS,Mouse,Mus musculus,PGD2 synthase,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds 96T
E0167b ELISA Bos taurus,Bovine,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS 96T
E0167b ELISA kit Bos taurus,Bovine,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS 96T
E0167r ELISA kit Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,Ptgds,Rat,Rattus norvegicus 96T
E0167p ELISA kit Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Pig,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS,Sus scrofa 96T
U0167p CLIA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Pig,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS,Sus scrofa 96T
E0167p ELISA Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Pig,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isomerase,PTGDS,Sus scrofa 96T
U0167h CLIA Beta-trace protein,Cerebrin-28,Glutathione-independent PGD synthase,Homo sapiens,Human,Lipocalin-type prostaglandin-D synthase,PDS,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin 96T
CSB-EL027234PL Plant solanesyl diphosphate synthase 2, chloroplastic(SPS2_SPPS)ELISA kit , Species , Sample Type 96T
U0167c CLIA Canis familiaris,Canis lupus familiaris,Dog,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-isom 96T
E0167c ELISA kit Canis familiaris,Canis lupus familiaris,Dog,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 96T
E0167c ELISA Canis familiaris,Canis lupus familiaris,Dog,Glutathione-independent PGD synthase,Lipocalin-type prostaglandin-D synthase,PGD2 synthase,PGDS,PGDS2,Prostaglandin-D2 synthase,Prostaglandin-H2 D-iso 96T
EIAAB06601 CDP-DAG synthase 2,CDP-DG synthase 2,CDP-diacylglycerol synthase 2,CDP-diglyceride pyrophosphorylase 2,CDP-diglyceride synthase 2,CDS 2,CDS2,CTP phosphatidate cytidylyltransferase 2,Homo sapiens,Human
EIAAB06597 CDP-DAG synthase 1,CDP-DG synthase 1,CDP-diacylglycerol synthase 1,CDP-diglyceride pyrophosphorylase 1,CDP-diglyceride synthase 1,CDS,CDS 1,CDS1,CTP phosphatidate cytidylyltransferase 1,Homo sapiens,H
EIAAB06600 Bos taurus,Bovine,CDP-DAG synthase 2,CDP-DG synthase 2,CDP-diacylglycerol synthase 2,CDP-diglyceride pyrophosphorylase 2,CDP-diglyceride synthase 2,CDS 2,CDS2,CTP phosphatidate cytidylyltransferase 2,
EIAAB06603 CDP-DAG synthase 2,CDP-DG synthase 2,CDP-diacylglycerol synthase 2,CDP-diglyceride pyrophosphorylase 2,CDP-diglyceride synthase 2,CDS 2,Cds2,CTP phosphatidate cytidylyltransferase 2,Mouse,Mus musculus
EIAAB06599 CDP-DAG synthase 1,CDP-DG synthase 1,CDP-diacylglycerol synthase 1,CDP-diglyceride pyrophosphorylase 1,CDP-diglyceride synthase 1,Cds,CDS 1,Cds1,CTP phosphatidate cytidylyltransferase 1,Mouse,Mus musc
10-288-22051F Prostaglandin-H2 D-isomerase - EC 5.3.99.2; Lipocalin-type prostaglandin-D synthase; Glutathione-independent PGD synthetase; Prostaglandin-D2 synthase; PGD2 synthase; PGDS2; PGDS; Beta-trace protein; 0.05 mg


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur