GENTAUR Molecular Products.

 ACRZ_ECOLI              Reviewed;          49 AA.
 P0AAW9; P75759;
 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
 11-OCT-2005, sequence version 1.
 13-FEB-2019, entry version 83.
 RecName: Full=Multidrug efflux pump accessory protein AcrZ {ECO:0000255|HAMAP-Rule:MF_01484};
 AltName: Full=AcrAB-TolC multidrug efflux pump accessory protein AcrZ {ECO:0000255|HAMAP-Rule:MF_01484};
 AltName: Full=Acridine resistance protein Z {ECO:0000255|HAMAP-Rule:MF_01484};
 Name=acrZ {ECO:0000255|HAMAP-Rule:MF_01484}; Synonyms=ybhT;
 OrderedLocusNames=b0762, JW5102;
 Escherichia coli (strain K12).
 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
 Enterobacteriaceae; Escherichia.
 NCBI_TaxID=83333;
 [1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
 PubMed=8905232; DOI=10.1093/dnares/3.3.137;
 Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
 Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
 Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
 Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
 Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
 Yano M., Horiuchi T.;
 "A 718-kb DNA sequence of the Escherichia coli K-12 genome
 corresponding to the 12.7-28.0 min region on the linkage map.";
 DNA Res. 3:137-155(1996).
 [2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=K12 / MG1655 / ATCC 47076;
 PubMed=9278503; DOI=10.1126/science.277.5331.1453;
 Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
 Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
 Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
 Mau B., Shao Y.;
 "The complete genome sequence of Escherichia coli K-12.";
 Science 277:1453-1462(1997).
 [3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
 PubMed=16738553; DOI=10.1038/msb4100049;
 Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
 Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
 "Highly accurate genome sequences of Escherichia coli K-12 strains
 MG1655 and W3110.";
 Mol. Syst. Biol. 2:E1-E5(2006).
 [4]
 SUBCELLULAR LOCATION, AND INDUCTION.
 STRAIN=K12 / MG1655 / ATCC 47076;
 PubMed=19121005; DOI=10.1111/j.1365-2958.2008.06495.x;
 Hemm M.R., Paul B.J., Schneider T.D., Storz G., Rudd K.E.;
 "Small membrane proteins found by comparative genomics and ribosome
 binding site models.";
 Mol. Microbiol. 70:1487-1501(2008).
 [5]
 INDUCTION.
 STRAIN=K12 / MG1655 / ATCC 47076;
 PubMed=19734316; DOI=10.1128/JB.00872-09;
 Hemm M.R., Paul B.J., Miranda-Rios J., Zhang A., Soltanzad N.,
 Storz G.;
 "Small stress response proteins in Escherichia coli: proteins missed
 by classical proteomic studies.";
 J. Bacteriol. 192:46-58(2010).
 [6]
 SUBCELLULAR LOCATION.
 STRAIN=K12 / MG1655 / ATCC 47076;
 PubMed=21778229; DOI=10.1074/jbc.M111.245696;
 Fontaine F., Fuchs R.T., Storz G.;
 "Membrane localization of small proteins in Escherichia coli.";
 J. Biol. Chem. 286:32464-32474(2011).
 [7]
 FUNCTION, INTERACTION WITH ACRB, SUBUNIT, INDUCTION, DISRUPTION
 PHENOTYPE, AND MUTAGENESIS OF GLY-30 AND 46-GLY--HIS-49.
 STRAIN=K12 / MG1655 / ATCC 47076;
 PubMed=23010927; DOI=10.1073/pnas.1210093109;
 Hobbs E.C., Yin X., Paul B.J., Astarita J.L., Storz G.;
 "Conserved small protein associates with the multidrug efflux pump
 AcrB and differentially affects antibiotic resistance.";
 Proc. Natl. Acad. Sci. U.S.A. 109:16696-16701(2012).
 -!- FUNCTION: AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex
     with a broad substrate specificity. This protein binds to AcrB and
     is required for efflux of some but not all substrates, suggesting
     it may influence the specificity of drug export.
     {ECO:0000255|HAMAP-Rule:MF_01484, ECO:0000269|PubMed:23010927}.
 -!- SUBUNIT: Part of the AcrA-AcrB-AcrZ-TolC efflux pump, interacts
     directly with AcrB. {ECO:0000255|HAMAP-Rule:MF_01484,
     ECO:0000269|PubMed:23010927}.
 -!- INTERACTION:
     P31224:acrB; NbExp=7; IntAct=EBI-6313593, EBI-551006;
 -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
     Rule:MF_01484, ECO:0000269|PubMed:19121005,
     ECO:0000269|PubMed:21778229}; Single-pass membrane protein
     {ECO:0000255|HAMAP-Rule:MF_01484, ECO:0000269|PubMed:19121005,
     ECO:0000269|PubMed:21778229}. Note=In sucrose cushions
     fractionates with both the inner and outer membranes.
 -!- INDUCTION: Constitutively expressed (PubMed:19121005), may be
     repressed at 45 degrees Celsius (PubMed:19121005) (at protein
     level). Positively regulated by MarA, Rob and SoxS transcriptional
     regulators. {ECO:0000269|PubMed:19121005,
     ECO:0000269|PubMed:19734316, ECO:0000269|PubMed:23010927}.
 -!- DISRUPTION PHENOTYPE: Becomes sensitive to some antibiotics
     (chloramphenicol, puromycin and tetracycline) but not to others
     (erythromycin, rifampicin and fusidic acid).
     {ECO:0000269|PubMed:23010927}.
 -!- SIMILARITY: Belongs to the AcrZ family. {ECO:0000255|HAMAP-
     Rule:MF_01484}.
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 EMBL; U00096; AAC73849.1; -; Genomic_DNA.
 EMBL; AP009048; BAA35426.2; -; Genomic_DNA.
 PIR; B64812; B64812.
 RefSeq; NP_415283.1; NC_000913.3.
 RefSeq; WP_000891515.1; NZ_LN832404.1.
 PDB; 4C48; X-ray; 3.30 A; C=1-49.
 PDB; 5NC5; X-ray; 3.20 A; F/G/H=1-49.
 PDB; 5NG5; EM; 6.50 A; M/N/O=1-49.
 PDB; 5O66; EM; 5.90 A; M/N/O=1-49.
 PDBsum; 4C48; -.
 PDBsum; 5NC5; -.
 PDBsum; 5NG5; -.
 PDBsum; 5O66; -.
 ProteinModelPortal; P0AAW9; -.
 SMR; P0AAW9; -.
 BioGrid; 4259947; 12.
 DIP; DIP-11423N; -.
 IntAct; P0AAW9; 2.
 STRING; 316385.ECDH10B_0830; -.
 TCDB; 2.A.6.2.2; the resistance-nodulation-cell division (rnd) superfamily.
 TCDB; 8.A.50.1.1; the acrz rnd auxillary subunit (acrz) family.
 PaxDb; P0AAW9; -.
 PRIDE; P0AAW9; -.
 EnsemblBacteria; AAC73849; AAC73849; b0762.
 EnsemblBacteria; BAA35426; BAA35426; BAA35426.
 GeneID; 945365; -.
 KEGG; ecj:JW5102; -.
 KEGG; eco:b0762; -.
 PATRIC; fig|1411691.4.peg.1516; -.
 EchoBASE; EB4074; -.
 EcoGene; EG14328; acrZ.
 HOGENOM; HOG000219534; -.
 BioCyc; EcoCyc:G6396-MONOMER; -.
 BioCyc; ECOL316407:JW5102-MONOMER; -.
 PRO; PR:P0AAW9; -.
 Proteomes; UP000000318; Chromosome.
 Proteomes; UP000000625; Chromosome.
 GO; GO:0009279; C:cell outer membrane; IDA:EcoCyc.
 GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
 GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
 GO; GO:0015238; F:drug transmembrane transporter activity; IMP:UniProtKB.
 GO; GO:0036460; P:cellular response to cell envelope stress; IMP:EcoCyc.
 GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
 HAMAP; MF_01484; AcrZ; 1.
 InterPro; IPR019702; AcrZ.
 Pfam; PF10766; AcrZ; 1.
 1: Evidence at protein level;
 3D-structure; Antibiotic resistance; Cell inner membrane;
 Cell membrane; Complete proteome; Membrane; Reference proteome;
 Stress response; Transmembrane; Transmembrane helix; Transport.
 CHAIN         1     49       Multidrug efflux pump accessory protein
                              AcrZ.
                              /FTId=PRO_0000013811.
 TOPO_DOM      1      7       Periplasmic. {ECO:0000305}.
 TRANSMEM      8     28       Helical. {ECO:0000255|HAMAP-
                              Rule:MF_01484}.
 TOPO_DOM     29     49       Cytoplasmic. {ECO:0000305}.
 MUTAGEN      30     30       G->R: Loss of chloramphenicol resistance,
                              does not bind AcrB (dominant-negative to
                              wild-type). Partially suppressed by AcrB
                              H526Y. {ECO:0000269|PubMed:23010927}.
 MUTAGEN      46     49       Missing: Loss of chloramphenicol
                              resistance, still binds AcrB (dominant-
                              negative to wild-type).
                              {ECO:0000269|PubMed:23010927}.
 HELIX         2     35       {ECO:0000244|PDB:4C48}.
 SEQUENCE   49 AA;  5300 MW;  1B1185D27F1F37F7 CRC64;
 MLELLKSLVF AVIMVPVVMA IILGLIYGLG EVFNIFSGVG KKDQPGQNH

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