Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Neurovirulence factor ICP34.5 (Infected cell protein 34.5) (protein gamma(1)34.5)

 ICP34_HHV1F             Reviewed;         263 AA.
P08353;
01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
01-OCT-1994, sequence version 2.
30-AUG-2017, entry version 65.
RecName: Full=Neurovirulence factor ICP34.5;
AltName: Full=Infected cell protein 34.5;
AltName: Full=protein gamma(1)34.5;
Name=RL1; Synonyms=ICP34.5;
Human herpesvirus 1 (strain F) (HHV-1) (Human herpes simplex virus 1).
Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
Alphaherpesvirinae; Simplexvirus.
NCBI_TaxID=10304;
NCBI_TaxID=9606; Homo sapiens (Human).
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3003394;
Chou J., Roizman B.;
"The terminal a sequence of the herpes simplex virus genome contains
the promoter of a gene located in the repeat sequences of the L
component.";
J. Virol. 57:629-637(1986).
[2]
SEQUENCE REVISION.
PubMed=2154589;
Chou J., Roizman B.;
"The herpes simplex virus 1 gene for ICP34.5, which maps in inverted
repeats, is conserved in several limited-passage isolates but not in
strain 17syn+.";
J. Virol. 64:1014-1020(1990).
[3]
FUNCTION, AND INTERACTION WITH HUMAN PPP1CA.
PubMed=9023344; DOI=10.1073/pnas.94.3.843;
He B., Gross M., Roizman B.;
"The gamma(1)34.5 protein of herpes simplex virus 1 complexes with
protein phosphatase 1alpha to dephosphorylate the alpha subunit of the
eukaryotic translation initiation factor 2 and preclude the shutoff of
protein synthesis by double-stranded RNA-activated protein kinase.";
Proc. Natl. Acad. Sci. U.S.A. 94:843-848(1997).
[4]
INTERACTION WITH HUMAN PPP1CA.
PubMed=9694816; DOI=10.1074/jbc.273.33.20737;
He B., Gross M., Roizman B.;
"The gamma134.5 protein of herpes simplex virus 1 has the structural
and functional attributes of a protein phosphatase 1 regulatory
subunit and is present in a high molecular weight complex with the
enzyme in infected cells.";
J. Biol. Chem. 273:20737-20743(1998).
[5]
MUTAGENESIS OF VAL-193 AND PHE-195.
PubMed=11882996; DOI=10.1006/viro.2001.1148;
Cheng G., Brett M.-E., He B.;
"Val193 and Phe195 of the gamma 1 34.5 protein of herpes simplex virus
1 are required for viral resistance to interferon-alpha/beta.";
Virology 290:115-120(2001).
[6]
FUNCTION.
PubMed=12072498; DOI=10.1128/JVI.76.14.6974-6986.2002;
Trgovcich J., Johnson D., Roizman B.;
"Cell surface major histocompatibility complex class II proteins are
regulated by the products of the gamma(1)34.5 and U(L)41 genes of
herpes simplex virus 1.";
J. Virol. 76:6974-6986(2002).
[7]
SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, NUCLEAR EXPORT
SIGNAL, AND MUTAGENESIS OF LEU-134; LEU-136; ARG-215 AND ARG-216.
PubMed=12186925; DOI=10.1128/JVI.76.18.9434-9445.2002;
Cheng G., Brett M.-E., He B.;
"Signals that dictate nuclear, nucleolar, and cytoplasmic shuttling of
the gamma(1)34.5 protein of herpes simplex virus type 1.";
J. Virol. 76:9434-9445(2002).
[8]
SUBCELLULAR LOCATION, AND INTERACTION WITH HOST PCNA.
PubMed=12907392; DOI=10.1080/13550280390218788;
Harland J., Dunn P., Cameron E., Conner J., Brown S.M.;
"The herpes simplex virus (HSV) protein ICP34.5 is a virion component
that forms a DNA-binding complex with proliferating cell nuclear
antigen and HSV replication proteins.";
J. Neurovirol. 9:477-488(2003).
[9]
REGION OF TRIPLET REPEATS.
PubMed=16099898; DOI=10.1099/vir.0.81033-0;
Jing X., He B.;
"Characterization of the triplet repeats in the central domain of the
gamma134.5 protein of herpes simplex virus 1.";
J. Gen. Virol. 86:2411-2419(2005).
[10]
INTERACTION WITH PP1.
PubMed=15705855; DOI=10.1126/science.1101902;
Boyce M., Bryant K.F., Jousse C., Long K., Harding H.P., Scheuner D.,
Kaufman R.J., Ma D., Coen D.M., Ron D., Yuan J.;
"A selective inhibitor of eIF2alpha dephosphorylation protects cells
from ER stress.";
Science 307:935-939(2005).
-!- FUNCTION: Contributes to HSV resistance to the antiviral effects
of alpha/beta interferon. Recruits the serine/threonine-protein
phosphatase PPP1CA/PP1-alpha to dephosphorylate the translation
initiation factor eIF-2A, thereby couteracting the host shutoff of
protein synthesis involving double-stranded RNA-dependent protein
kinase EIF2AK2/PKR. Also down-modulates the host MHC class II
proteins cell surface expression. Acts as a neurovirulence factor
that has a profound effect on the growth of the virus in central
nervous system tissue, probably through its ability to maintain an
environment favorable for viral replication.
{ECO:0000269|PubMed:12072498, ECO:0000269|PubMed:9023344}.
-!- SUBUNIT: Interacts with human PPP1CA to form a high-molecular-
weight complex that dephosphorylates eIF2-alpha. Binds to host
PCNA, which may release host cells from growth arrest and
facilitate viral replication. {ECO:0000269|PubMed:12907392,
ECO:0000269|PubMed:15705855, ECO:0000269|PubMed:9023344,
ECO:0000269|PubMed:9694816}.
-!- SUBCELLULAR LOCATION: Host cytoplasm. Host nucleus, host
nucleolus. Virion {ECO:0000250}. Note=At early times in infection,
colocalizes with PCNA and replication proteins in cell nuclei,
before accumulating in the cytoplasm by 8 to 12 hours post-
infection. The effects on the host cell are mediated by de novo-
synthesized ICP34.5, the virion-derived population being either
non-functional or present in very low amounts (By similarity).
{ECO:0000250}.
-!- DOMAIN: The triplet repeats region may play a role in modulating
virus egress.
-!- MISCELLANEOUS: ICP34.5 is detected as early as 3 hpi prior to
viral replication but reaches maximal levels late in infection.
ICP34.5 gene is therefore classified as gamma-1 or leaky late gene
(By similarity). {ECO:0000250}.
-!- MISCELLANEOUS: The phosphatase activity of the ICP34.5-PP1 complex
toward EIF2S1 is specifically inhibited by Salubrinal, which
inhibits viral replication.
-!- SIMILARITY: Belongs to the PPP1R15 family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; M12240; AAA45794.1; ALT_SEQ; Genomic_DNA.
EMBL; M33699; AAA45790.1; -; Genomic_DNA.
PIR; A27768; WMBE38.
ELM; P08353; -.
IntAct; P08353; 2.
MINT; MINT-8293225; -.
GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0044196; C:host cell nucleolus; IEA:UniProtKB-SubCell.
GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
GO; GO:0039586; P:modulation by virus of host PP1 activity; IEA:UniProtKB-KW.
GO; GO:0009405; P:pathogenesis; IEA:UniProtKB-KW.
GO; GO:0039521; P:suppression by virus of host autophagy; IEA:UniProtKB-KW.
GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
InterPro; IPR019523; Prot_Pase1_reg-su15A/B_C.
Pfam; PF10488; PP1c_bdg; 1.
1: Evidence at protein level;
Host cytoplasm; Host nucleus; Host-virus interaction;
Inhibition of host autophagy by virus;
Inhibition of host innate immune response by virus;
Inhibition of host interferon signaling pathway by virus;
Inhibition of host RLR pathway by virus;
Modulation of host PP1 activity by virus; Repeat; Viral immunoevasion;
Virion; Virulence.
CHAIN 1 263 Neurovirulence factor ICP34.5.
/FTId=PRO_0000115807.
REPEAT 161 163 1. {ECO:0000269|PubMed:16099898}.
REPEAT 164 166 2. {ECO:0000269|PubMed:16099898}.
REPEAT 167 169 3. {ECO:0000269|PubMed:16099898}.
REPEAT 170 172 4. {ECO:0000269|PubMed:16099898}.
REPEAT 173 175 5. {ECO:0000269|PubMed:16099898}.
REPEAT 176 178 6. {ECO:0000269|PubMed:16099898}.
REPEAT 179 181 7. {ECO:0000269|PubMed:16099898}.
REPEAT 182 184 8. {ECO:0000269|PubMed:16099898}.
REPEAT 185 187 9. {ECO:0000269|PubMed:16099898}.
REPEAT 188 190 10. {ECO:0000269|PubMed:16099898}.
REGION 1 16 Required for nucleolar localization.
REGION 161 190 10 X 3 AA tandem repeats of A-T-P.
REGION 190 203 Binding to PPP1CA.
REGION 205 263 Important for interferon resistance.
MOTIF 128 137 Nuclear export signal.
MOTIF 215 233 Bipartite nuclear localization signal.
COMPBIAS 3 8 Poly-Arg.
COMPBIAS 10 190 Pro-rich.
COMPBIAS 75 81 Poly-Asp.
MUTAGEN 134 134 L->A: Complete loss of cytoplasmic
localization; when associated with A-136.
{ECO:0000269|PubMed:12186925}.
MUTAGEN 136 136 L->A: Complete loss of cytoplasmic
localization; when associated with A-134.
{ECO:0000269|PubMed:12186925}.
MUTAGEN 193 193 V->E: Loss of interferon resistance; when
associated with F-195.
{ECO:0000269|PubMed:11882996}.
MUTAGEN 195 195 F->L: Loss of interferon resistance; when
associated with V-193.
{ECO:0000269|PubMed:11882996}.
MUTAGEN 215 215 R->A: Decrease in nuclear localization;
when associated with A-216.
{ECO:0000269|PubMed:12186925}.
MUTAGEN 216 216 R->A: Decrease in nuclear localization;
when associated with A-215.
{ECO:0000269|PubMed:12186925}.
SEQUENCE 263 AA; 27533 MW; F5084106A08A8CB1 CRC64;
MARRRRHRGP RRPRPPGPTG AVPTAQSQVT STPNSEPAVR SAPAAAPPPP PASGPPPSCS
LLLRQWLHVP ESASDDDDDD DWPDSPPPEP APEARPTAAA PRPRSPPPGA GPGGGANPSH
PPSRPFRLPP RLALRLRVTA EHLARLRLRR AGGEGAPEPP ATPATPATPA TPATPATPAT
PATPATPATP ARVRFSPHVR VRHLVVWASA ARLARRGSWA RERADRARFR RRVAEAEAVI
GPCLGPEARA RALARGAGPA NSV


Related products :

Catalog number Product name Quantity
EIAAB32070 Fibroblast growth factor-inducible protein 13,FIN13,Fin13,Mouse,Mus musculus,PP2C-gamma,Ppm1c,Ppm1g,Protein phosphatase 1C,Protein phosphatase 1G,Protein phosphatase 2C isoform gamma,Protein phosphata
EIAAB38927 Gamma-soluble NSF attachment protein,Homo sapiens,Human,NAPG,N-ethylmaleimide-sensitive factor attachment protein gamma,SNAPG,SNAP-gamma
EIAAB38925 Gamma-soluble NSF attachment protein,Mouse,Mus musculus,Napg,N-ethylmaleimide-sensitive factor attachment protein gamma,Snapg,SNAP-gamma
EIAAB38926 Bos taurus,Bovine,Gamma-soluble NSF attachment protein,NAPG,N-ethylmaleimide-sensitive factor attachment protein gamma,SNAPG,SNAP-gamma
18-661-15200 Protein BEX3 - Brain-expressed X-linked protein 3; p75NTR-associated cell death executor; Nerve growth factor receptor-associated protein 1; Ovarian granulosa cell 13.0 kDa protein HGR74 Polyclonal 0.1 mg
EIAAB43884 E3 ubiquitin-protein ligase TRIM33,Ectodermin homolog,Homo sapiens,Human,KIAA1113,Protein Rfg7,RET-fused gene 7 protein,RFG7,TIF1G,TIF1-gamma,Transcription intermediary factor 1-gamma,TRIM33,Tripartit
10-288-22050F Gamma-aminobutyric acid receptor-associated protein-like 2 - GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 0.1 mg
15-288-22050F Gamma-aminobutyric acid receptor-associated protein-like 2 - GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 0.1 mg
15-288-22050F Gamma-aminobutyric acid receptor-associated protein-like 2 - GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 0.05 mg
10-288-22050F Gamma-aminobutyric acid receptor-associated protein-like 2 - GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; GEF-2; General protein transport factor p16; MAP1 light chain 0.05 mg
18-003-43285 Transcription factor AP-2 gamma - AP2-gamma; Activating enhancer-binding protein 2 gamma; AP-2.2 Polyclonal 0.1 mg Protein A
20-272-191626 AP2 gamma - Mouse monoclonal [AP2g 6E4_4] to AP2 gamma; AP2-gamma; Activating enhancer-binding protein 2 gamma; Transcription factor ERF-1 Monoclonal 0.1 mg
27-789 IVNS1ABP is a novel human protein that interacts with the influenza A virus. Nonstructural NS1 protein is relocalized in the nuclei of infected cells. 0.1 mg
EIAAB43885 E3 ubiquitin-protein ligase TRIM33,Ectodermin homolog,Kiaa1113,Mouse,Mus musculus,TIF1-gamma,Transcription intermediary factor 1-gamma,Trim33,Tripartite motif-containing protein 33
EIAAB06706 C_EBP gamma,CCAAT_enhancer-binding protein gamma,Cebpg,GPE1-binding protein,GPE1-BP,Granulocyte colony-stimulating factor promoter element 1-binding protein,IG_EBP-1,Immunoglobulin enhancer-binding pr
EIAAB11411 Dlk2,DLK-2,Egfl9,EGF-like protein 9,Endothelial cell-specific protein S-1,Epidermal growth factor-like protein 9,Mouse,Mus musculus,Protein delta homolog 2
EIAAB11408 Dlk2,DLK-2,Egfl9,EGF-like protein 9,Endothelial cell-specific protein S-1,Epidermal growth factor-like protein 9,Protein delta homolog 2,Rat,Rattus norvegicus
EIAAB32069 Homo sapiens,Human,PP2C-gamma,PPM1C,PPM1G,Protein phosphatase 1C,Protein phosphatase 1G,Protein phosphatase 2C isoform gamma,Protein phosphatase magnesium-dependent 1 gamma
SCH-PRP34 RECOMBINANT RAT STEM CELL FACTOR , Product Type Recombinant Protein, Specificity STEM CELL FACTOR, Target Species Rat, Host N_A, Format Rec. Protein, Isotypes , Applications FN, Clone 10 µg
PRP34 RECOMBINANT RAT STEM CELL FACTOR , Product Type Recombinant Protein, Specificity STEM CELL FACTOR, Target Species Rat, Host N_A, Format Rec. Protein, Isotypes , Applications FN, Clone 10 µg
EIAAB33234 Homo sapiens,Human,Peroxin-11C,Peroxisomal biogenesis factor 11C,Peroxisomal membrane protein 11C,PEX11C,PEX11G,PEX11-gamma,Protein PEX11 homolog gamma
EIAAB33235 Mouse,Mus musculus,Peroxin-11C,Peroxisomal biogenesis factor 11C,Peroxisomal membrane protein 11C,Pex11c,Pex11g,PEX11-gamma,Protein PEX11 homolog gamma
orb82070 Mouse IFN-gamma, recombinant protein Interferon gamma (IFN-gamma) Recombinant is a purified cytokine_growth factor. For research use only. 100
PP-PPM1G-050 PPM1G, Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phos 50
orb82072 Rat IFN-gamma, recombinant protein Interferon gamma (IFN-gamma) Recombinant is a purified cytokine_growth factor. For research use only. 100


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur