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Nitrile hydratase subunit alpha (NHase) (Nitrilase) (EC 4.2.1.84)

 NHAA_PSECL              Reviewed;         200 AA.
P27764;
01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 3.
05-DEC-2018, entry version 84.
RecName: Full=Nitrile hydratase subunit alpha;
Short=NHase;
Short=Nitrilase;
EC=4.2.1.84;
Name=nthA;
Pseudomonas chlororaphis (Pseudomonas aureofaciens).
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
Pseudomonadaceae; Pseudomonas.
NCBI_TaxID=333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-26 AND
164-178.
STRAIN=B23;
PubMed=2013568; DOI=10.1128/jb.173.8.2465-2472.1991;
Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H.,
Beppu T.;
"Cloning and characterization of genes responsible for metabolism of
nitrile compounds from Pseudomonas chlororaphis B23.";
J. Bacteriol. 173:2465-2472(1991).
-!- FUNCTION: NHase catalyzes the hydration of various nitrile
compounds to the corresponding amides. Industrial production of
acrylamide is now being developed using some of the enzymes of
this class.
-!- CATALYTIC ACTIVITY:
Reaction=an aliphatic amide = a nitrile + H2O;
Xref=Rhea:RHEA:12673, ChEBI:CHEBI:15377, ChEBI:CHEBI:18379,
ChEBI:CHEBI:65285; EC=4.2.1.84;
-!- COFACTOR:
Name=Fe(3+); Xref=ChEBI:CHEBI:29034;
Note=Binds 1 Fe(3+) ion per subunit.;
-!- ACTIVITY REGULATION: Inactivated by oxidation of Cys-110 to a
sulfenic acid.
-!- SUBUNIT: Heterodimer of an alpha and a beta chain.
-!- PTM: Oxidation on Cys-108 is essential for the activity.
{ECO:0000250}.
-!- PTM: Oxidation on Cys-110 stabilizes the Fe-NO ligand coordinated
in the inactive form.
-!- SIMILARITY: Belongs to the nitrile hydratase subunit alpha family.
{ECO:0000305}.
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EMBL; D90216; BAA14245.1; -; Genomic_DNA.
PIR; A42725; A42725.
ProteinModelPortal; P27764; -.
SMR; P27764; -.
BioCyc; MetaCyc:MONOMER-15567; -.
GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
Gene3D; 3.90.330.10; -; 1.
InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
InterPro; IPR023900; CN_Hdrtase_asu/SCN_Hdrlase_gsu.
InterPro; IPR018141; Nitrile_hydratase_asu.
Pfam; PF02979; NHase_alpha; 1.
PIRSF; PIRSF001426; NHase_alpha; 1.
ProDom; PD007559; CN_Hdrtase_asu/SCN_Hdrlase_gsu; 1.
SUPFAM; SSF56209; SSF56209; 1.
TIGRFAMs; TIGR01323; nitrile_alph; 1.
1: Evidence at protein level;
Direct protein sequencing; Iron; Lyase; Metal-binding; Oxidation.
INIT_MET 1 1 Removed. {ECO:0000269|PubMed:2013568}.
CHAIN 2 200 Nitrile hydratase subunit alpha.
/FTId=PRO_0000186820.
METAL 105 105 Iron. {ECO:0000250}.
METAL 108 108 Iron. {ECO:0000250}.
METAL 109 109 Iron. {ECO:0000250}.
METAL 110 110 Iron. {ECO:0000250}.
MOD_RES 108 108 Cysteine sulfinic acid (-SO2H).
{ECO:0000250}.
MOD_RES 110 110 Cysteine sulfenic acid (-SOH).
{ECO:0000250}.
SEQUENCE 200 AA; 22117 MW; 12A80064CFCB0EFE CRC64;
MSTSISTTAT PSTPGERAWA LFQVLKSKEL IPEGYVEQLT QLMAHDWSPE NGARVVAKAW
VDPQFRALLL KDGTAACAQF GYTGPQGEYI VALEDTPGVK NVIVCSLCSC TNWPVLGLPP
EWYKGFEFRA RLVREGRTVL RELGTELPSD TVIKVWDTSA ESRYLVLPQR PEGSEHMSEE
QLQQLVTKDV LIGVALPRVG


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