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PTS system N,N'-diacetylchitobiose-specific EIIA component (EIIA-Chb) (EIII-Chb) (IIIcel) (N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component)

 PTQA_ECOLI              Reviewed;         116 AA.
P69791; P17335; Q47092; Q47093; Q47094; Q57128;
10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
10-MAY-2005, sequence version 1.
28-MAR-2018, entry version 107.
RecName: Full=PTS system N,N'-diacetylchitobiose-specific EIIA component {ECO:0000303|PubMed:2092358};
AltName: Full=EIIA-Chb {ECO:0000303|PubMed:2092358};
AltName: Full=EIII-Chb {ECO:0000303|PubMed:2179047};
AltName: Full=IIIcel {ECO:0000303|PubMed:2179047};
AltName: Full=N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component {ECO:0000303|PubMed:2092358};
Name=chbA {ECO:0000303|PubMed:9405618};
Synonyms=celC {ECO:0000303|PubMed:2179047};
OrderedLocusNames=b1736, JW1725;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND GENE NAME.
STRAIN=K12;
PubMed=2179047;
Parker L.L., Hall B.G.;
"Characterization and nucleotide sequence of the cryptic cel operon of
Escherichia coli K12.";
Genetics 124:455-471(1990).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=28, 35, 37, 50, 51, 58, 6, 61, 66, 69, and ECOR 1;
PubMed=1630305;
Hall B.G., Sharp P.M.;
"Molecular population genetics of Escherichia coli: DNA sequence
diversity at the celC, crr, and gutB loci of natural isolates.";
Mol. Biol. Evol. 9:654-665(1992).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=9097039; DOI=10.1093/dnares/3.6.363;
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome
corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
FUNCTION, AND ACTIVE SITE.
PubMed=2092358; DOI=10.1016/0923-2508(90)90079-6;
Reizer J., Reizer A., Saier M.H. Jr.;
"The cellobiose permease of Escherichia coli consists of three
proteins and is homologous to the lactose permease of Staphylococcus
aureus.";
Res. Microbiol. 141:1061-1067(1990).
[7]
IDENTIFICATION OF CHB OPERON.
PubMed=9405618; DOI=10.1073/pnas.94.26.14367;
Keyhani N.O., Roseman S.;
"Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-
diacetylchitobiose, by expressing the cel operon.";
Proc. Natl. Acad. Sci. U.S.A. 94:14367-14371(1997).
[8]
FUNCTION, CATALYTIC ACTIVITY, INDUCTION, AND SUBSTRATE SPECIFICITY.
PubMed=10913117; DOI=10.1074/jbc.M001043200;
Keyhani N.O., Wang L.-X., Lee Y.C., Roseman S.;
"The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by
Escherichia coli and is transported/phosphorylated by the
phosphoenolpyruvate:glycose phosphotransferase system.";
J. Biol. Chem. 275:33084-33090(2000).
[9]
FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, AND PHOSPHORYLATION
AT HIS-89.
PubMed=10913118; DOI=10.1074/jbc.M001044200;
Keyhani N.O., Boudker O., Roseman S.;
"Isolation and characterization of IIAChb, a soluble protein of the
enzyme II complex required for the transport/phosphorylation of N, N'-
diacetylchitobiose in Escherichia coli.";
J. Biol. Chem. 275:33091-33101(2000).
[10]
SUBUNIT.
PubMed=10913119; DOI=10.1074/jbc.M001045200;
Keyhani N.O., Bacia K., Roseman S.;
"The transport/phosphorylation of N,N'-diacetylchitobiose in
Escherichia coli. Characterization of phospho-IIB(Chb) and of a
potential transition state analogue in the phosphotransfer reaction
between the proteins IIA(Chb) and IIB(Chb).";
J. Biol. Chem. 275:33102-33109(2000).
[11]
SUBUNIT.
PubMed=10913122; DOI=10.1074/jbc.M001717200;
Keyhani N.O., Rodgers M.E., Demeler B., Hansen J.C., Roseman S.;
"Analytical sedimentation of the IIAChb and IIBChb proteins of the
Escherichia coli N,N'-diacetylchitobiose phosphotransferase system.
Demonstration of a model phosphotransfer transition state complex.";
J. Biol. Chem. 275:33110-33115(2000).
[12]
STRUCTURE BY NMR OF 14-116 OF MUTANT LEU-92, MUTAGENESIS OF ASP-92,
COFACTOR, ACTIVE SITE, SUBUNIT, AND PHOSPHORYLATION AT HIS-89.
PubMed=15654077; DOI=10.1074/jbc.M414300200;
Tang C., Williams D.C. Jr., Ghirlando R., Clore G.M.;
"Solution structure of enzyme IIA(Chitobiose) from the N,N'-
diacetylchitobiose branch of the Escherichia coli phosphotransferase
system.";
J. Biol. Chem. 280:11770-11780(2005).
[13]
STRUCTURE BY NMR OF 14-116 OF MUTANT GLU-89, ACTIVE SITE, AND SUBUNIT.
PubMed=19959833; DOI=10.1074/jbc.M109.080937;
Jung Y.S., Cai M., Clore G.M.;
"Solution structure of the IIAChitobiose-IIBChitobiose complex of the
N,N'-diacetylchitobiose branch of the Escherichia coli
phosphotransferase system.";
J. Biol. Chem. 285:4173-4184(2010).
[14]
STRUCTURE BY NMR OF 14-116 OF MUTANT GLU-89 IN COMPLEX WITH THE
HISTIDINE PHOSPHOCARRIER PROTEIN HPR, ACTIVE SITE, SUBUNIT, AND
PHOSPHORYLATION AT HIS-89.
PubMed=22593574; DOI=10.1074/jbc.M112.371492;
Jung Y.S., Cai M., Clore G.M.;
"Solution structure of the IIAChitobiose-HPr complex of the N,N'-
diacetylchitobiose branch of the Escherichia coli phosphotransferase
system.";
J. Biol. Chem. 287:23819-23829(2012).
-!- FUNCTION: The phosphoenolpyruvate-dependent sugar
phosphotransferase system (sugar PTS), a major carbohydrate active
transport system, catalyzes the phosphorylation of incoming sugar
substrates concomitantly with their translocation across the cell
membrane. The enzyme II ChbABC PTS system is involved in the
transport of the chitin disaccharide N,N'-diacetylchitobiose
(GlcNAc2) (PubMed:10913118, PubMed:10913117). Also able to use
N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).
{ECO:0000269|PubMed:10913117, ECO:0000269|PubMed:10913118,
ECO:0000305|PubMed:2092358}.
-!- COFACTOR:
Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Evidence={ECO:0000269|PubMed:10913118,
ECO:0000305|PubMed:15654077};
Note=Can also use copper and nickel with lower efficiency.
{ECO:0000269|PubMed:10913118};
-!- SUBUNIT: Forms a complex with ChbB (EIIB) (PubMed:10913119,
PubMed:10913122, PubMed:19959833). ChbA is a homotrimer
(PubMed:10913122, PubMed:15654077, PubMed:19959833,
PubMed:22593574). The interface of the homotrimer is stabilized by
metal cations such as magnesium, copper or nickel
(PubMed:15654077). {ECO:0000269|PubMed:10913119,
ECO:0000269|PubMed:10913122, ECO:0000269|PubMed:15654077,
ECO:0000269|PubMed:19959833, ECO:0000269|PubMed:22593574,
ECO:0000305|PubMed:10913118}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- INDUCTION: By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose
(Me-TCB). {ECO:0000269|PubMed:10913117}.
-!- DOMAIN: The PTS EIIA type-3 domain is phosphorylated by phospho-
HPr on a histidyl residue. Then, it transfers the phosphoryl group
to the PTS EIIB type-3 domain. {ECO:0000255|PROSITE-
ProRule:PRU00418}.
-!- CAUTION: Was originally (PubMed:2179047) characterized as part of
a cryptic cel operon for a cellobiose degradation system. The Cel+
phenotype is due to mutations making expression chitobiose-
independent and altering the substrate specificity.
{ECO:0000305|PubMed:2179047}.
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EMBL; X52890; CAA37071.1; -; Genomic_DNA.
EMBL; M93570; AAA23551.1; -; Genomic_DNA.
EMBL; M93571; AAA23553.1; -; Genomic_DNA.
EMBL; M93572; AAA23554.1; -; Genomic_DNA.
EMBL; M93573; AAA23559.1; -; Genomic_DNA.
EMBL; M93574; AAA23556.1; -; Genomic_DNA.
EMBL; M93575; AAA23552.1; -; Genomic_DNA.
EMBL; M93576; AAA23560.1; -; Genomic_DNA.
EMBL; M93577; AAA23557.1; -; Genomic_DNA.
EMBL; M93591; AAA23561.1; -; Genomic_DNA.
EMBL; M93592; AAA23555.1; -; Genomic_DNA.
EMBL; M93593; AAA23558.1; -; Genomic_DNA.
EMBL; U00096; AAC74806.1; -; Genomic_DNA.
EMBL; AP009048; BAA15517.1; -; Genomic_DNA.
PIR; H64932; H64932.
RefSeq; NP_416250.1; NC_000913.3.
RefSeq; WP_000968919.1; NZ_LN832404.1.
PDB; 1WCR; NMR; -; A/B/C=14-116.
PDB; 2LRK; NMR; -; A/B/C=14-116.
PDB; 2LRL; NMR; -; A/B/C=14-116.
PDB; 2WWV; NMR; -; A/B/C=14-116.
PDB; 2WY2; NMR; -; A/B/C=14-116.
PDBsum; 1WCR; -.
PDBsum; 2LRK; -.
PDBsum; 2LRL; -.
PDBsum; 2WWV; -.
PDBsum; 2WY2; -.
ProteinModelPortal; P69791; -.
SMR; P69791; -.
BioGrid; 4259134; 12.
DIP; DIP-9265N; -.
IntAct; P69791; 11.
STRING; 316385.ECDH10B_1874; -.
TCDB; 4.A.3.2.1; the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.
iPTMnet; P69791; -.
PaxDb; P69791; -.
PRIDE; P69791; -.
EnsemblBacteria; AAC74806; AAC74806; b1736.
EnsemblBacteria; BAA15517; BAA15517; BAA15517.
GeneID; 946244; -.
KEGG; ecj:JW1725; -.
KEGG; eco:b1736; -.
PATRIC; fig|1411691.4.peg.520; -.
EchoBASE; EB0140; -.
EcoGene; EG10142; chbA.
eggNOG; ENOG4105VCP; Bacteria.
eggNOG; COG1447; LUCA.
HOGENOM; HOG000224338; -.
InParanoid; P69791; -.
KO; K02759; -.
OMA; MGLIING; -.
PhylomeDB; P69791; -.
BioCyc; EcoCyc:CELC-MONOMER; -.
BioCyc; MetaCyc:CELC-MONOMER; -.
EvolutionaryTrace; P69791; -.
PRO; PR:P69791; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0005829; C:cytosol; IDA:EcoCyc.
GO; GO:0090566; F:protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity; IDA:EcoCyc.
GO; GO:1902815; P:N,N'-diacetylchitobiose import; IDA:EcoCyc.
GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IDA:EcoCyc.
GO; GO:0051260; P:protein homooligomerization; IDA:EcoCyc.
CDD; cd00215; PTS_IIA_lac; 1.
InterPro; IPR003188; PTS_IIA_lac/cel.
InterPro; IPR036542; PTS_IIA_lac/cel_sf.
PANTHER; PTHR34382; PTHR34382; 1.
Pfam; PF02255; PTS_IIA; 1.
PIRSF; PIRSF000699; PTS_IILac_III; 1.
SUPFAM; SSF46973; SSF46973; 1.
TIGRFAMs; TIGR00823; EIIA-LAC; 1.
PROSITE; PS51095; PTS_EIIA_TYPE_3; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Cytoplasm; Phosphoprotein;
Phosphotransferase system; Reference proteome; Sugar transport;
Transferase; Transport.
CHAIN 1 116 PTS system N,N'-diacetylchitobiose-
specific EIIA component.
/FTId=PRO_0000186494.
DOMAIN 15 113 PTS EIIA type-3. {ECO:0000255|PROSITE-
ProRule:PRU00418}.
ACT_SITE 89 89 Tele-phosphohistidine intermediate.
{ECO:0000305|PubMed:15654077,
ECO:0000305|PubMed:19959833,
ECO:0000305|PubMed:2092358,
ECO:0000305|PubMed:22593574}.
MOD_RES 89 89 Phosphohistidine; by HPr.
{ECO:0000255|PROSITE-ProRule:PRU00418,
ECO:0000305|PubMed:10913118,
ECO:0000305|PubMed:15654077,
ECO:0000305|PubMed:22593574}.
VARIANT 14 14 A -> V (in strain: ECOR 1).
VARIANT 52 52 A -> T (in strain: ECOR 61).
VARIANT 59 59 M -> I (in strain: ECOR 50).
MUTAGEN 92 92 D->L: Eliminates the need for a metal
cation at the homotrimer interface by
substituting hydrophobic methyl-methyl
interactions in place of the metal
cation. {ECO:0000269|PubMed:15654077}.
CONFLICT 43 44 KQ -> NR (in Ref. 1; CAA37071).
{ECO:0000305}.
HELIX 15 43 {ECO:0000244|PDB:1WCR}.
HELIX 47 74 {ECO:0000244|PDB:1WCR}.
STRAND 77 79 {ECO:0000244|PDB:1WCR}.
HELIX 85 114 {ECO:0000244|PDB:1WCR}.
SEQUENCE 116 AA; 12748 MW; F2951DC6700FA8A9 CRC64;
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA KAMMDQSRMA
LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA RELITELIEL HEKLKA


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