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PTS system glucose-specific EIIA component (EIIA-Glc) (EIII-Glc) (Glucose-specific phosphotransferase enzyme IIA component)

 PTGA_SALTY              Reviewed;         169 AA.
P0A283; P02908;
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 2.
25-APR-2018, entry version 79.
RecName: Full=PTS system glucose-specific EIIA component {ECO:0000303|PubMed:6086327};
AltName: Full=EIIA-Glc {ECO:0000303|PubMed:6086327};
AltName: Full=EIII-Glc {ECO:0000303|PubMed:6086327};
AltName: Full=Glucose-specific phosphotransferase enzyme IIA component {ECO:0000303|PubMed:6754734};
Name=crr; OrderedLocusNames=STM2433;
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Salmonella.
NCBI_TaxID=99287;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6086327;
Nelson S.O., Schuitema A.R.J., Benne R., van der Ploeg L.H.T.,
Plijter J.S., Aan F., Postma P.W.;
"Molecular cloning, sequencing, and expression of the crr gene: the
structural gene for IIIGlc of the bacterial PEP:glucose
phosphotransferase system.";
EMBO J. 3:1587-1593(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=LT2 / SGSC1412 / ATCC 700720;
PubMed=11677609; DOI=10.1038/35101614;
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M.,
Waterston R., Wilson R.K.;
"Complete genome sequence of Salmonella enterica serovar Typhimurium
LT2.";
Nature 413:852-856(2001).
[3]
FUNCTION, AND DISRUPTION PHENOTYPE.
PubMed=789369;
Saier M.H. Jr., Roseman S.;
"Sugar transport. The crr mutation: its effect on repression of enzyme
synthesis.";
J. Biol. Chem. 251:6598-6605(1976).
[4]
FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
PubMed=6754734;
Meadow N.D., Roseman S.;
"Sugar transport by the bacterial phosphotransferase system. Isolation
and characterization of a glucose-specific phosphocarrier protein
(IIIGlc) from Salmonella typhimurium.";
J. Biol. Chem. 257:14526-14537(1982).
[5]
FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
SUBSTRATE SPECIFICITY.
PubMed=6292227;
Stock J.B., Waygood E.B., Meadow N.D., Postma P.W., Roseman S.;
"Sugar transport by the bacterial phosphotransferase system. The
glucose receptors of the Salmonella typhimurium phosphotransferase
system.";
J. Biol. Chem. 257:14543-14552(1982).
[6]
FUNCTION.
PubMed=17158705; DOI=10.1128/MMBR.00024-06;
Deutscher J., Francke C., Postma P.W.;
"How phosphotransferase system-related protein phosphorylation
regulates carbohydrate metabolism in bacteria.";
Microbiol. Mol. Biol. Rev. 70:939-1031(2006).
-!- FUNCTION: The phosphoenolpyruvate-dependent sugar
phosphotransferase system (sugar PTS), a major carbohydrate active
transport system, catalyzes the phosphorylation of incoming sugar
substrates concomitantly with their translocation across the cell
membrane. The enzyme II complex composed of PtsG and Crr is
involved in glucose transport (PubMed:6754734, PubMed:6292227). It
can also phosphorylate mannose, methyl alpha-glucoside and 2-
deoxy-glucose (PubMed:6292227). The non-phosphorylated EIII-Glc is
an inhibitor for uptake of certain sugars such as maltose,
melibiose, lactose, and glycerol. Phosphorylated EIII-Glc,
however, may be an activator for adenylate cyclase
(PubMed:789369). {ECO:0000269|PubMed:6292227,
ECO:0000269|PubMed:6754734, ECO:0000269|PubMed:789369,
ECO:0000305|PubMed:17158705}.
-!- COFACTOR:
Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
Evidence={ECO:0000250|UniProtKB:P69783};
Note=Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc
ion is important for dimerization. {ECO:0000250|UniProtKB:P69783};
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=3.4 uM for methyl alpha-glucoside (with phospho-IIIGlc Slow)
{ECO:0000269|PubMed:6754734};
KM=6 uM for methyl alpha-glucoside {ECO:0000269|PubMed:6292227};
KM=10 uM for glucose {ECO:0000269|PubMed:6292227};
KM=40 uM for mannose {ECO:0000269|PubMed:6292227};
KM=200 uM for 2-deoxy-glucose {ECO:0000269|PubMed:6292227};
Vmax=126 umol/min/mg enzyme with methyl alpha-glucoside as
substrate {ECO:0000269|PubMed:6292227};
Vmax=126 umol/min/mg enzyme with glucose as substrate
{ECO:0000269|PubMed:6292227};
Vmax=10 umol/min/mg enzyme with mannose as substrate
{ECO:0000269|PubMed:6292227};
Vmax=10 umol/min/mg enzyme with 2-deoxy-glucose as substrate
{ECO:0000269|PubMed:6292227};
-!- SUBUNIT: Heterodimer with glycerol kinase (glpk).
{ECO:0000250|UniProtKB:P69783}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a
histidyl residue. Then, it transfers the phosphoryl group to the
EIIB domain. {ECO:0000255|PROSITE-ProRule:PRU00416}.
-!- DISRUPTION PHENOTYPE: In mutants defective in enzyme I and
histidine-containing phosphate carrier protein (HPr), cells
lacking this gene are able to grow on the non-PTS compounds such
as glycerol, maltose, melibiose, mannose 6-phosphate, and alpha-
glycerol phosphate. {ECO:0000269|PubMed:789369}.
-!- MISCELLANEOUS: Two forms of III-Glc (called IIIGlc Slow and IIIGlc
Fast) are present in living cells. IIIGlc Fast, derived from
IIIGlc Slow by cleavage of the seven NH2-terminal amino acids, is
present only in trace quantities and has slight activity (only 2-
3% of phospho-IIIGlc Slow) in the phosphorylation of sugar. Both
forms accept phosphate from phosphoenolpyruvate via Enzyme I and
HPr, however only IIIGlc Slow participates in the phosphorylation
of glucose or methyl alpha-D-glucoside. IIIGlc Fast may play a
role in the regulation of non-PTS sugar transport systems.
{ECO:0000269|PubMed:6754734}.
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EMBL; X05210; CAA28837.1; -; Genomic_DNA.
EMBL; AE006468; AAL21327.1; -; Genomic_DNA.
PIR; A03405; WQEB3T.
RefSeq; NP_461368.1; NC_003197.2.
RefSeq; WP_000522253.1; NC_003197.2.
ProteinModelPortal; P0A283; -.
SMR; P0A283; -.
IntAct; P0A283; 1.
STRING; 99287.STM2433; -.
PaxDb; P0A283; -.
PRIDE; P0A283; -.
EnsemblBacteria; AAL21327; AAL21327; STM2433.
GeneID; 1253955; -.
GeneID; 29379035; -.
KEGG; stm:STM2433; -.
PATRIC; fig|99287.12.peg.2571; -.
eggNOG; ENOG4105C5Y; Bacteria.
eggNOG; COG2190; LUCA.
HOGENOM; HOG000224567; -.
KO; K02777; -.
OMA; HTKHAVG; -.
PhylomeDB; P0A283; -.
BioCyc; SENT99287:G1FZD-2456-MONOMER; -.
PRO; PR:P0A283; -.
Proteomes; UP000001014; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
InterPro; IPR011055; Dup_hybrid_motif.
InterPro; IPR001127; PTS_EIIA_1_perm.
Pfam; PF00358; PTS_EIIA_1; 1.
SUPFAM; SSF51261; SSF51261; 1.
TIGRFAMs; TIGR00830; PTBA; 1.
PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
1: Evidence at protein level;
Complete proteome; Cytoplasm; Kinase; Metal-binding; Phosphoprotein;
Phosphotransferase system; Reference proteome; Sugar transport;
Transferase; Transport; Zinc.
INIT_MET 1 1 Removed. {ECO:0000250|UniProtKB:P69783}.
CHAIN 2 169 PTS system glucose-specific EIIA
component.
/FTId=PRO_0000186535.
DOMAIN 39 143 PTS EIIA type-1. {ECO:0000255|PROSITE-
ProRule:PRU00416}.
ACT_SITE 91 91 Tele-phosphohistidine intermediate; for
EIIA activity.
{ECO:0000250|UniProtKB:P69783,
ECO:0000255|PROSITE-ProRule:PRU00416}.
METAL 76 76 Zinc; shared with glycerol kinase.
{ECO:0000250|UniProtKB:P69783}.
METAL 91 91 Zinc; shared with glycerol kinase.
{ECO:0000250|UniProtKB:P69783}.
SITE 76 76 Important for phospho-donor activity.
{ECO:0000250|UniProtKB:P69783}.
MOD_RES 91 91 Phosphohistidine; by HPr.
{ECO:0000250|UniProtKB:P69783}.
SEQUENCE 169 AA; 18247 MW; 451098233E8E1479 CRC64;
MGLFDKLKSL VSDDKKDTGT IEIVAPLSGE IVNIEDVPDV VFAEKIVGDG IAIKPTGNKM
VAPVDGTIGK IFETNHAFSI ESDSGIELFV HFGIDTVELK GEGFKRIAEE GQRVKVGDPV
IEFDLPLLEE KAKSTLTPVV ISNMDEIKEL IKLSGSVTVG ETPVIRIKK


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