Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Penicillin-binding protein 1A (PBP-1a) (PBP1a) [Includes: Penicillin-insensitive transglycosylase (EC 2.4.1.129) (Peptidoglycan TGase); Penicillin-sensitive transpeptidase (EC 3.4.16.4) (DD-transpeptidase)]

 PBPA_ECOLI              Reviewed;         850 AA.
P02918; P76688; Q2M761;
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
21-JUL-1986, sequence version 1.
28-MAR-2018, entry version 174.
RecName: Full=Penicillin-binding protein 1A;
Short=PBP-1a;
Short=PBP1a;
Includes:
RecName: Full=Penicillin-insensitive transglycosylase;
EC=2.4.1.129 {ECO:0000269|PubMed:7006606};
AltName: Full=Peptidoglycan TGase;
Includes:
RecName: Full=Penicillin-sensitive transpeptidase;
EC=3.4.16.4 {ECO:0000269|PubMed:7006606};
AltName: Full=DD-transpeptidase;
Name=mrcA; Synonyms=ponA; OrderedLocusNames=b3396, JW3359;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=3882429; DOI=10.1111/j.1432-1033.1985.tb08768.x;
Broome-Smith J.K., Edelman A., Yousif S., Spratt B.G.;
"The nucleotide sequences of the ponA and ponB genes encoding
penicillin-binding protein 1A and 1B of Escherichia coli K12.";
Eur. J. Biochem. 147:437-446(1985).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
PROTEIN SEQUENCE OF 452-480.
STRAIN=K12;
PubMed=3920658; DOI=10.1073/pnas.82.7.1999;
Keck W., Glauner B., Schwarz U., Broome-Smith J.K., Spratt B.G.;
"Sequences of the active-site peptides of three of the high-Mr
penicillin-binding proteins of Escherichia coli K-12.";
Proc. Natl. Acad. Sci. U.S.A. 82:1999-2003(1985).
[5]
FUNCTION, CATALYTIC ACTIVITY, PATHWAY, AND SUBCELLULAR LOCATION.
STRAIN=K12;
PubMed=7006606; DOI=10.1016/S0006-291X(80)80166-5;
Ishino F., Mitsui K., Tamaki S., Matsuhashi M.;
"Dual enzyme activities of cell wall peptidoglycan synthesis,
peptidoglycan transglycosylase and penicillin-sensitive
transpeptidase, in purified preparations of Escherichia coli
penicillin-binding protein 1A.";
Biochem. Biophys. Res. Commun. 97:287-293(1980).
[6]
REVIEW.
PubMed=9841666;
Goffin C., Ghuysen J.-M.;
"Multimodular penicillin-binding proteins: an enigmatic family of
orthologs and paralogs.";
Microbiol. Mol. Biol. Rev. 62:1079-1093(1998).
-!- FUNCTION: Cell wall formation. Synthesis of cross-linked
peptidoglycan from the lipid intermediates. The enzyme has a
penicillin-insensitive transglycosylase N-terminal domain
(formation of linear glycan strands) and a penicillin-sensitive
transpeptidase C-terminal domain (cross-linking of the peptide
subunits). {ECO:0000269|PubMed:7006606}.
-!- CATALYTIC ACTIVITY: (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-
Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-
Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-
diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-
Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl
diphosphate. {ECO:0000269|PubMed:7006606}.
-!- CATALYTIC ACTIVITY: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-
D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are
N-acyl substituents of D-alanine. {ECO:0000269|PubMed:7006606}.
-!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
{ECO:0000269|PubMed:7006606}.
-!- INTERACTION:
P45464:lpoA; NbExp=4; IntAct=EBI-1126191, EBI-557795;
-!- SUBCELLULAR LOCATION: Cell inner membrane
{ECO:0000269|PubMed:7006606}; Single-pass type II membrane protein
{ECO:0000255}.
-!- SIMILARITY: In the N-terminal section; belongs to the
glycosyltransferase 51 family. {ECO:0000305}.
-!- SIMILARITY: In the C-terminal section; belongs to the
transpeptidase family. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA58193.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; X02164; CAA26100.1; -; Genomic_DNA.
EMBL; U18997; AAA58193.1; ALT_INIT; Genomic_DNA.
EMBL; U00096; AAC76421.2; -; Genomic_DNA.
EMBL; AP009048; BAE77895.1; -; Genomic_DNA.
PIR; G65134; ZPECPA.
RefSeq; NP_417855.4; NC_000913.3.
RefSeq; WP_001336003.1; NZ_LN832404.1.
ProteinModelPortal; P02918; -.
SMR; P02918; -.
BioGrid; 4262992; 302.
DIP; DIP-10251N; -.
IntAct; P02918; 8.
STRING; 316385.ECDH10B_3571; -.
ChEMBL; CHEMBL1813; -.
DrugBank; DB01414; Cefacetrile.
DrugBank; DB01327; Cefazolin.
DrugBank; DB00274; Cefmetazole.
DrugBank; DB01328; Cefonicid.
DrugBank; DB01329; Cefoperazone.
DrugBank; DB01331; Cefoxitin.
DrugBank; DB00430; Cefpiramide.
DrugBank; DB01333; Cefradine.
DrugBank; DB00438; Ceftazidime.
DrugBank; DB01415; Ceftibuten.
DrugBank; DB01332; Ceftizoxime.
DrugBank; DB06211; Doripenem.
DrugBank; DB00303; Ertapenem.
DrugBank; DB01598; Imipenem.
DrugBank; DB04570; Latamoxef.
CAZy; GT51; Glycosyltransferase Family 51.
PaxDb; P02918; -.
PRIDE; P02918; -.
EnsemblBacteria; AAC76421; AAC76421; b3396.
EnsemblBacteria; BAE77895; BAE77895; BAE77895.
GeneID; 947907; -.
KEGG; ecj:JW3359; -.
KEGG; eco:b3396; -.
PATRIC; fig|1411691.4.peg.3334; -.
EchoBASE; EB0741; -.
EcoGene; EG10748; mrcA.
eggNOG; ENOG4108JQC; Bacteria.
eggNOG; COG5009; LUCA.
HOGENOM; HOG000041138; -.
InParanoid; P02918; -.
KO; K05366; -.
OMA; LAQMAMI; -.
PhylomeDB; P02918; -.
BioCyc; EcoCyc:EG10748-MONOMER; -.
BioCyc; MetaCyc:EG10748-MONOMER; -.
UniPathway; UPA00219; -.
PRO; PR:P02918; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoliWiki.
GO; GO:0008658; F:penicillin binding; IDA:EcoCyc.
GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IDA:EcoCyc.
GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IDA:EcoCyc.
GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
GO; GO:0009252; P:peptidoglycan biosynthetic process; IDA:EcoCyc.
GO; GO:0008360; P:regulation of cell shape; IGI:EcoCyc.
GO; GO:0046677; P:response to antibiotic; IDA:EcoCyc.
Gene3D; 1.10.3810.10; -; 1.
InterPro; IPR012338; Beta-lactam/transpept-like.
InterPro; IPR001264; Glyco_trans_51.
InterPro; IPR023346; Lysozyme-like_dom_sf.
InterPro; IPR036950; PBP_transglycosylase.
InterPro; IPR031376; PCB_OB.
InterPro; IPR001460; PCN-bd_Tpept.
Pfam; PF17092; PCB_OB; 1.
Pfam; PF00912; Transgly; 1.
Pfam; PF00905; Transpeptidase; 1.
SUPFAM; SSF53955; SSF53955; 1.
SUPFAM; SSF56601; SSF56601; 4.
1: Evidence at protein level;
Antibiotic resistance; Carboxypeptidase; Cell inner membrane;
Cell membrane; Cell shape; Cell wall biogenesis/degradation;
Complete proteome; Direct protein sequencing; Glycosyltransferase;
Hydrolase; Membrane; Multifunctional enzyme; Peptidoglycan synthesis;
Protease; Reference proteome; Signal-anchor; Transferase;
Transmembrane; Transmembrane helix.
CHAIN 1 850 Penicillin-binding protein 1A.
/FTId=PRO_0000083164.
TOPO_DOM 1 5 Cytoplasmic. {ECO:0000255}.
TRANSMEM 6 26 Helical; Signal-anchor for type II
membrane protein. {ECO:0000255}.
TOPO_DOM 27 850 Periplasmic. {ECO:0000255}.
REGION 48 216 Transglycosylase.
REGION 400 710 Transpeptidase.
ACT_SITE 86 86 Proton donor; for transglycosylase
activity. {ECO:0000250|UniProtKB:P02919}.
ACT_SITE 465 465 Acyl-ester intermediate; for
transpeptidase activity.
{ECO:0000250|UniProtKB:P02919}.
SEQUENCE 850 AA; 93636 MW; 0A54C6B28783E34B CRC64;
MKFVKYFLIL AVCCILLGAG SIYGLYRYIE PQLPDVATLK DVRLQIPMQI YSADGELIAQ
YGEKRRIPVT LDQIPPEMVK AFIATEDSRF YEHHGVDPVG IFRAASVALF SGHASQGAST
ITQQLARNFF LSPERTLMRK IKEVFLAIRI EQLLTKDEIL ELYLNKIYLG YRAYGVGAAA
QVYFGKTVDQ LTLNEMAVIA GLPKAPSTFN PLYSMDRAVA RRNVVLSRML DEGYITQQQF
DQTRTEAINA NYHAPEIAFS APYLSEMVRQ EMYNRYGESA YEDGYRIYTT ITRKVQQAAQ
QAVRNNVLDY DMRHGYRGPA NVLWKVGESA WDNNKITDTL KALPTYGPLL PAAVTSANPQ
QATAMLADGS TVALSMEGVR WARPYRSDTQ QGPTPRKVTD VLQTGQQIWV RQVGDAWWLA
QVPEVNSALV SINPQNGAVM ALVGGFDFNQ SKFNRATQAL RQVGSNIKPF LYTAAMDKGL
TLASMLNDVP ISRWDASAGS DWQPKNSPPQ YAGPIRLRQG LGQSKNVVMV RAMRAMGVDY
AAEYLQRFGF PAQNIVHTES LALGSASFTP MQVARGYAVM ANGGFLVDPW FISKIENDQG
GVIFEAKPKV ACPECDIPVI YGDTQKSNVL ENNDVEDVAI SREQQNVSVP MPQLEQANQA
LVAKTGAQEY APHVINTPLA FLIKSALNTN IFGEPGWQGT GWRAGRDLQR RDIGGKTGTT
NSSKDAWFSG YGPGVVTSVW IGFDDHRRNL GHTTASGAIK DQISGYEGGA KSAQPAWDAY
MKAVLEGVPE QPLTPPPGIV TVNIDRSTGQ LANGGNSREE YFIEGTQPTQ QAVHEVGTTI
IDNGEAQELF


Related products :

Catalog number Product name Quantity
AS11 1717 Antibody: Penicillin G, Total IgG (0.5mg), Immunogen: penicillin G , Host: rabbit , polyclonal, Confirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 0.5 mg
AS11 1657 Antibody: Penicillin G, Total IgG (2.5mg), Immunogen: penicillin G , Host: rabbit , polyclonal, Confirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 2.5 mg
AS11 1731 Antibody: Penicillin G, Serum (1ml), Immunogen: penicillin G , Host: rabbit , polyclonal, Confirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 percen 1 ml
AS11 1642 Antibody: Penicillin G, Serum (0.1ml), Immunogen: penicillin G, Host: rabbit, polyclonal, Confirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 percent ), 0.1 ml
AS11 1646 Antibody: Penicillin V, Total IgG (1 mg), Immunogen: penicillin V , Host: rabbit , polyclonal, Confirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 percen 1 mg
AS11 1751 Antibody: Penicillin V (0.2 mg total IgG), Immunogen: penicillin V , Host: rabbit , polyclonal, Confirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 perce 2.5 mg
AS11 1657 Antibody: Penicillin G, Total IgG (2.5mg) , Host: rabbit , polyclonal, Comfirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 percent ), dicloxacillin (70 2.5 mg
AS11 1661 Antibody: Penicillin V, Serum (0.1 ml), Immunogen: penicillin V , Host: rabbit , polyclonal, Confirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 percent 0.1 ml
AS11 1661 Antibody: Penicillin V, Serum (0.1 ml) , Host: rabbit , polyclonal, Comfirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 percent ), penicillin G (20 percen 0.1 ml
AS11 1694 Antibody: Penicillin V, Serum (1 ml), Immunogen: penicillin V , Host: rabbit , polyclonal, Confirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 percent ), 1 ml
AS11 1751 Antibody: Penicillin V (0.2 mg total IgG) , Host: rabbit , polyclonal, Comfirmed reactivity: penicillin V (100 percent ), cloxacillin (90 percent ), dicloxacillin (80 percent ), penicillin G (20 per 2.5 mg
AS11 1642 Antibody: Penicillin G, Serum (0.1ml) , Host: rabbit, polyclonal, Comfirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 percent ), dicloxacillin (70 percen 0.1 ml
AS11 1731 Antibody: Penicillin G, Serum (1ml) , Host: rabbit , polyclonal, Comfirmed reactivity: penicillin G (100 percent ), penicillin acid (90 percent ), cloxacillin (110 percent ), dicloxacillin (70 perce 1 ml
AE81025PE Penicillin G (benzyl penicillin) ELISA Kit 96T
69-57-8 Penicillin Series (Penicillin G Procaine_P - 1g
7219-9959 MOUSE ANTI PENICILLIN, Product Type Monoclonal Antibody, Specificity PENICILLIN, Target Species Fungal, Host Mouse, Format Purified, Isotypes IgG1, Applications E, Clone Pen_9 0.2 mg
SCH-7219-9959 MOUSE ANTI PENICILLIN, Product Type Monoclonal Antibody, Specificity PENICILLIN, Target Species Fungal, Host Mouse, Format Purified, Isotypes IgG1, Applications E, Clone Pen_9 0.2 mg
SCH-7220-0004 SHEEP ANTI PENICILLIN, Product Type Polyclonal Antibody, Specificity PENICILLIN, Target Species Fungal, Host Sheep, Format Serum, Isotypes Polyclonal IgG, Applications E, R, Clone 0.2 ml
7220-0004 SHEEP ANTI PENICILLIN, Product Type Polyclonal Antibody, Specificity PENICILLIN, Target Species Fungal, Host Sheep, Format Serum, Isotypes Polyclonal IgG, Applications E, R, Clone 0.2 ml
Mab-00819 Penicillin, Penicillin
Mab-00818 Penicillin, Penicillin
1406-05-9 Penicillin Penicillin 1g
orb62246 procaine penicillin procaine penicillin For research use only. 500 g
30034-13-0 Penicillin G sulfoxide p-methoxybenzyl e Penicillin G sulfoxide p- 1g
B280 PM Indicator Agar (Penicillin In Milk Indicator Agar) USE : For rapid detection of trace amounts of Penicillin in milk as per AOAC. 5x500gm


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur