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Pesticidal crystal protein Cry1Ab (130 kDa crystal protein) (Crystaline entomocidal protoxin) (Insecticidal delta-endotoxin CryIA(b))

 CR1AB_BACTK             Reviewed;        1155 AA.
P0A370; P06577; P06578; P09663; P09666; P09667; P21257; Q45789;
01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
01-JAN-1988, sequence version 1.
23-MAY-2018, entry version 55.
RecName: Full=Pesticidal crystal protein Cry1Ab;
AltName: Full=130 kDa crystal protein;
AltName: Full=Crystaline entomocidal protoxin;
AltName: Full=Insecticidal delta-endotoxin CryIA(b);
Name=cry1Ab; Synonyms=bt2, cry-1-2, cry1A(b), cryIA(b), cryIC1;
Bacillus thuringiensis subsp. kurstaki.
Plasmid pUCBtS93.
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
Bacillus cereus group.
NCBI_TaxID=29339;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1;
PubMed=3557124; DOI=10.1016/0378-1119(86)90357-4;
Geiser M., Schweitzer S., Grimm C.;
"The hypervariable region in the genes coding for entomopathogenic
crystal proteins of Bacillus thuringiensis: nucleotide sequence of the
kurhd1 gene of subsp. kurstaki HD1.";
Gene 48:109-118(1986).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1;
PubMed=3011746; DOI=10.1128/jb.166.3.801-811.1986;
Thorne L., Garduno F., Thompson T., Decker D., Zounes M., Wild M.,
Walfield A.M., Pollock T.J.;
"Structural similarity between the lepidoptera- and diptera-specific
insecticidal endotoxin genes of Bacillus thuringiensis subsp.
'kurstaki' and 'israelensis'.";
J. Bacteriol. 166:801-811(1986).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1;
AGRICOLA=IND87016057;
Kondo S., Tamura N., Kunitate A., Hattori M., Akashi A., Ohmori I.;
"Cloning and nucleotide sequencing of two insecticidal delta-endotoxin
genes from Bacillus thuringiensis var. kurstaki HD-1 DNA.";
Agric. Biol. Chem. 51:455-463(1987).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=HD-1;
DOI=10.1038/nbt0887-807;
Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G.,
McCormick S.M., Niedermeyer J.G., Dean D.A., Kusano-Kretzmer K.,
Mayer E.J., Rochester D.E., Rogers S.G., Fraley R.T.;
"Insect tolerant transgenic tomato plants.";
Biotechnology (N.Y.) 5:807-813(1987).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NRD-12;
DOI=10.1016/0168-1656(87)90061-7;
Hefford M.A., Brousseau R., Prefontaine G., Hanna Z., Condie J.A.,
Lau P.C.K.;
"Sequence of a lepidopteran toxin gene of Bacillus thuringiensis subsp
kurstaki NRD-12.";
J. Biotechnol. 6:307-322(1987).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=S93; PLASMID=pUCBtS93;
DOI=10.1139/w99-032;
Silva-Werneck J.O., De-Souza M.T., Dias J.M.C.S., Ribeiro B.M.;
"Characterization of Bacillus thuringiensis subsp. kurstaki strain S93
effective against the Fall armyworm, Spodoptera frugiperda and cloning
of a cry1Ab gene.";
Can. J. Microbiol. 45:464-471(1999).
-!- FUNCTION: Promotes colloidosmotic lysis by binding to the midgut
epithelial cells of many lepidopteran larvae.
-!- DEVELOPMENTAL STAGE: The crystal protein is produced during
sporulation and is accumulated both as an inclusion and as part of
the spore coat.
-!- BIOTECHNOLOGY: Introduced by genetic manipulation and expressed in
insect-resistant maize by Monsanto, Northrup King and Ciba Geigy.
-!- MISCELLANEOUS: Toxic segment of the protein is located in the N-
terminus.
-!- MISCELLANEOUS: In strain S93 this is encoded on plasmid pUCBtS39.
{ECO:0000269|Ref.6}.
-!- SIMILARITY: Belongs to the delta endotoxin family. {ECO:0000305}.
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EMBL; M15271; AAA22561.1; -; Genomic_DNA.
EMBL; M12661; AAA22613.1; -; Genomic_DNA.
EMBL; D00117; BAA00071.1; -; Genomic_DNA.
EMBL; M37263; AAA22420.1; -; Genomic_DNA.
EMBL; AF059670; AAC64003.1; -; Genomic_DNA.
EMBL; A03793; CAA00303.1; -; Unassigned_DNA.
EMBL; A09398; CAA00840.1; -; Unassigned_DNA.
PIR; A29125; A29125.
PIR; A29838; A29838.
PIR; A90025; JD0002.
PIR; I39838; I39838.
RefSeq; WP_000369819.1; NZ_PGEH01000375.1.
ProteinModelPortal; P0A370; -.
SMR; P0A370; -.
TCDB; 1.C.2.1.3; the channel-forming Delta-endotoxin insecticidal crystal protein (icp) family.
GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
Gene3D; 1.20.190.10; -; 1.
Gene3D; 2.100.10.10; -; 1.
Gene3D; 2.60.120.260; -; 2.
InterPro; IPR008979; Galactose-bd-like_sf.
InterPro; IPR005638; Pest_crys_C.
InterPro; IPR005639; Pest_crys_N.
InterPro; IPR036716; Pest_crys_N_sf.
InterPro; IPR001178; Pest_cryst_cen_dom.
InterPro; IPR036399; Pest_cryst_cen_dom_sf.
Pfam; PF03944; Endotoxin_C; 1.
Pfam; PF00555; Endotoxin_M; 1.
Pfam; PF03945; Endotoxin_N; 1.
SUPFAM; SSF49785; SSF49785; 1.
SUPFAM; SSF51096; SSF51096; 1.
SUPFAM; SSF56849; SSF56849; 1.
1: Evidence at protein level;
Genetically modified food; Plasmid; Sporulation; Toxin.
CHAIN 1 1155 Pesticidal crystal protein Cry1Ab.
/FTId=PRO_0000174023.
CONFLICT 207 207 A -> R (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 382 406 VLDGTEFAYGTSSNLPSAVYRKSGT -> GPDGGRICLWNL
LKFGQPPYTEKAEP (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 410 410 L -> P (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 430 432 LSH -> CLAY (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 437 437 R -> Y (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 447 447 I -> V (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 452 458 MFSWIHR -> NDSSWTYC (in Ref. 2;
AAA22613). {ECO:0000305}.
CONFLICT 461 461 E -> N (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 463 465 NNI -> GDV (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 479 486 STNLGSGT -> LQSWLWN (in Ref. 2;
AAA22613). {ECO:0000305}.
CONFLICT 492 492 P -> L (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 501 505 RRTSP -> EELT (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 542 542 D -> H (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 568 569 TV -> HL (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 665 665 K -> E (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 675 676 KR -> NG (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 703 703 S -> N (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 712 712 D -> H (in Ref. 2; AAA22613).
{ECO:0000305}.
CONFLICT 832 832 A -> E (in Ref. 4; no nucleotide entry).
{ECO:0000305}.
CONFLICT 836 836 N -> I (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 842 843 EK -> GRA (in Ref. 4; no nucleotide
entry). {ECO:0000305}.
CONFLICT 1016 1016 E -> T (in Ref. 5; AAA22420).
{ECO:0000305}.
CONFLICT 1060 1060 E -> G (in Ref. 5; AAA22420).
{ECO:0000305}.
SEQUENCE 1155 AA; 130624 MW; 43461A64C7AC7CAF CRC64;
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ
RWGFDAATIN SRYNDLTRLI GNYTDHAVRW YNTGLERVWG PDSRDWIRYN QFRRELTLTV
LDIVSLFPNY DSRTYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIEGS IRSPHLMDIL
NSITIYTDAH RGEYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIPSSQ ITQIPLTKST
NLGSGTSVVK GPGFTGGDIL RRTSPGQIST LRVNITAPLS QRYRVRIRYA STTNLQFHTS
IDGRPINQGN FSATMSSGSN LQSGSFRTVG FTTPFNFSNG SSVFTLSAHV FNSGNEVYID
RIEFVPAEVT FEAEYDLERA QKAVNELFTS SNQIGLKTDV TDYHIDQVSN LVECLSDEFC
LDEKKELSEK VKHAKRLSDE RNLLQDPNFR GINRQLDRGW RGSTDITIQG GDDVFKENYV
TLLGTFDECY PTYLYQKIDE SKLKAYTRYQ LRGYIEDSQD LEIYLIRYNA KHETVNVPGT
GSLWPLSAPS PIGKCAHHSH HFSLDIDVGC TDLNEDLGVW VIFKIKTQDG HARLGNLEFL
EEKPLVGEAL ARVKRAEKKW RDKREKLEWE TNIVYKEAKE SVDALFVNSQ YDRLQADTNI
AMIHAADKRV HSIREAYLPE LSVIPGVNAA IFEELEGRIF TAFSLYDARN VIKNGDFNNG
LSCWNVKGHV DVEEQNNHRS VLVVPEWEAE VSQEVRVCPG RGYILRVTAY KEGYGEGCVT
IHEIENNTDE LKFSNCVEEE VYPNNTVTCN DYTATQEEYE GTYTSRNRGY DGAYESNSSV
PADYASAYEE KAYTDGRRDN PCESNRGYGD YTPLPAGYVT KELEYFPETD KVWIEIGETE
GTFIVDSVEL LLMEE


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Catalog number Product name Quantity
at166 Recombinant Pesticidal crystal protein cry1Ab 1 mg
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abx109060 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (HRP) 100 μg
abx106231 Polyclonal Rabbit Pesticidal crystal protein cry1Ab Antibody (Biotin) 100 μg
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abx109061 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (HRP) 100 μg
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abx106232 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (Biotin) 100 μg
abx106234 Polyclonal Rabbit Pesticidal crystal protein cry3Aa Antibody (Biotin) 100 μg
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abx107644 Polyclonal Rabbit Pesticidal crystal protein cry1Ac Antibody (FITC) 100 μg
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MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers 1 kit
MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers kit
MSK-2 Crystal Harvesting Sampler Kit, contains 100 mounts for crystal harvesting and data collection; 20 Dual Thickness MicroMounts, 20 MicroLoops LD, 20 MicroMesh, 20 MicroLoop E, and 20 MicroGrippers kit
MSK-1 Crystal Harvesting Sampler Kit, contains 40 mounts for crystal harvesting and data collection; 10 Dual Thickness MicroMounts, 10 MicroLoops, 5 MicroMesh, 5 MicroLoop LD, 5 MicroLoop E, and 5 MicroGrip 1 kit
MSK-1 Crystal Harvesting Sampler Kit, contains 40 mounts for crystal harvesting and data collection; 10 Dual Thickness MicroMounts, 10 MicroLoops, 5 MicroMesh, 5 MicroLoop LD, 5 MicroLoop E, and 5 MicroGrip kit
orb60731 Crystal violet Crystal violet is a triarylmethane dye. For research use only. 10 mg


 

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