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Poly(A) polymerase catalytic subunit (EC 2.7.7.19) (Poly(A) polymerase large subunit) (PAP-L)

 PAP1_LSDVN              Reviewed;         474 AA.
Q91MX5; Q77GP9; Q8JTW7;
23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
28-MAR-2018, entry version 52.
RecName: Full=Poly(A) polymerase catalytic subunit;
EC=2.7.7.19;
AltName: Full=Poly(A) polymerase large subunit;
Short=PAP-L;
Name=PAPL; OrderedLocusNames=LSDV032, LD032, LW032;
Lumpy skin disease virus (isolate Bovine/Kenya/Neethling 2490/1958)
(LSDV) (Lumpy skin disease virus (isolate NI-2490)).
Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae;
Capripoxvirus.
NCBI_TaxID=376849;
NCBI_TaxID=9913; Bos taurus (Bovine).
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Isolate Neethling 2490;
PubMed=11435593; DOI=10.1128/JVI.75.15.7122-7130.2001;
Tulman E.R., Afonso C.L., Lu Z., Zsak L., Kutish G.F., Rock D.L.;
"Genome of lumpy skin disease virus.";
J. Virol. 75:7122-7130(2001).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Isolate Neethling vaccine LW 1959, and
Isolate Neethling Warmbaths LW;
PubMed=12827464; DOI=10.1007/s00705-003-0102-0;
Kara P.D., Afonso C.L., Wallace D.B., Kutish G.F., Abolnik C., Lu Z.,
Vreede F.T., Taljaard L.C.F., Zsak A., Viljoen G.J., Rock D.L.;
"Comparative sequence analysis of the South African vaccine strain and
two virulent field isolates of Lumpy skin disease virus.";
Arch. Virol. 148:1335-1356(2003).
-!- FUNCTION: Polymerase that creates the 3'-poly(A) tail of mRNA's.
-!- CATALYTIC ACTIVITY: ATP + RNA(n) = diphosphate + RNA(n+1).
-!- SUBUNIT: Heterodimer of a large (catalytic) subunit and a small
(regulatory) subunit. {ECO:0000250}.
-!- SIMILARITY: Belongs to the poxviridae poly(A) polymerase catalytic
subunit family. {ECO:0000305}.
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EMBL; AF325528; AAK84993.1; -; Genomic_DNA.
EMBL; AF409137; AAN02600.1; -; Genomic_DNA.
EMBL; AF409138; AAN02757.1; -; Genomic_DNA.
RefSeq; NP_150466.1; NC_003027.1.
ProteinModelPortal; Q91MX5; -.
SMR; Q91MX5; -.
GeneID; 921671; -.
KEGG; vg:921671; -.
KO; K21315; -.
Proteomes; UP000126568; Genome.
Proteomes; UP000127252; Genome.
Proteomes; UP000156762; Genome.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0004652; F:polynucleotide adenylyltransferase activity; IEA:UniProtKB-EC.
GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
Gene3D; 1.20.1270.320; -; 1.
Gene3D; 3.30.460.60; -; 1.
InterPro; IPR037265; PolyA_pol_cat_sf.
InterPro; IPR024231; Pox_polyA_pol_nucTrfase.
InterPro; IPR038419; Pox_polyA_pol_nucTrfase_sf.
InterPro; IPR004976; Poxviridae_polyA_pol_cat.
InterPro; IPR024397; Poxvirus_polyA_pol_cat_C.
InterPro; IPR024398; Poxvirus_polyA_pol_cat_N.
InterPro; IPR038337; Poxvirus_polyA_pol_cat_N_sf.
Pfam; PF03296; Pox_polyA_pol; 1.
Pfam; PF12629; Pox_polyA_pol_C; 1.
Pfam; PF12630; Pox_polyA_pol_N; 1.
PIRSF; PIRSF015693; VAC-48L_nuct; 1.
SUPFAM; SSF160957; SSF160957; 1.
3: Inferred from homology;
ATP-binding; Complete proteome; mRNA processing; Nucleotide-binding;
Reference proteome; Transcription; Transferase.
CHAIN 1 474 Poly(A) polymerase catalytic subunit.
/FTId=PRO_0000308934.
ACT_SITE 193 193 {ECO:0000250}.
ACT_SITE 195 195 {ECO:0000250}.
VARIANT 123 123 T -> A (in strain: isolate Neethling
vaccine LW 1959).
VARIANT 270 270 A -> T (in strain: isolate Neethling
vaccine LW 1959).
SEQUENCE 474 AA; 54565 MW; 3BD4209BEBDD14F7 CRC64;
MNDKIVVDVL KLYLKRDPSL TEFHMLKSQY KNIQRVNIFN KEIFISLIKK NKKKFFSDIK
SSPSDIKRSI LTYFSKQENT YSIGKLYTII ELQSILVTTY TDVLGILTTK GPDLFTSNIY
YNTSSMQSLA RDALNFMNVA QMTDKTMGRH NVSSLVDNVN SLMEEYLRRH NKNCICYGSY
SLHLLNPDVK YGDIDILQTN SRTFLIDLAF LIKFITGLNV VLLKVPYLKN YMVLKDQNDS
HIIDSFNIRQ DTMQSIPKVL IDNIYIVDPA LQLMSMLKMF SQIDRLEDLA KNPEKLTVRL
ATLLEYVRVN YGINFISNQT KKNNMPMYSV IDIDKRIITV DTSNYDFPFK KCLVYLDESS
LSSDILDLNA DDAIDFENVS NSAFLINNNI LYTYFSNTIL MKSKTDIHEI SSRGLSAHIL
IYQILTKGDI IKPLTDIVNS LIGVEKNPIY DIIPRDKKSG KHGIIDIEKD IITH


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