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Probable transcriptional regulator WhiBTM4

 WHIB_BPMT4              Reviewed;          76 AA.
Q9ZX29;
28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
01-MAY-1999, sequence version 1.
20-DEC-2017, entry version 51.
RecName: Full=Probable transcriptional regulator WhiBTM4;
Name=whiBTM4; Synonyms=49; ORFNames=TM4_49;
Mycobacterium phage TM4 (Mycobacteriophage TM4).
Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae;
Tm4virus.
NCBI_TaxID=88870;
NCBI_TaxID=1763; Mycobacterium.
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=10645443; DOI=10.1054/tuld.1998.0007;
Ford M.E., Stenstrom C., Hendrix R.W., Hatfull G.F.;
"Mycobacteriophage TM4: genome structure and gene expression.";
Tuber. Lung Dis. 79:63-73(1998).
[2]
FUNCTION, COFACTOR, SUBUNIT, DNA-BINDING, INDUCTION, DISRUPTION
PHENOTYPE, AND MUTAGENESIS OF CYS-36; CYS-39; CYS-45; LEU-55; VAL-56;
VAL-58; TRP-59 AND ARG-67.
PubMed=20545868; DOI=10.1111/j.1365-2958.2010.07235.x;
Rybniker J., Nowag A., van Gumpel E., Nissen N., Robinson N., Plum G.,
Hartmann P.;
"Insights into the function of the WhiB-like protein of
mycobacteriophage TM4--a transcriptional inhibitor of WhiB2.";
Mol. Microbiol. 77:642-657(2010).
-!- FUNCTION: A dominant-negative regulator of endogenous whiB2
expression. Low level expression in liquid culture in M.smegmatis
leads to elongated and highly branched cells, with multiple
nucleoids, similar to the effects seen in deletion experiments for
whiB2 in M.smegmatis, as well as loss of acid-fastness. High level
expression is lethal. Represses expression of endogenous whiB2.
The apo-form binds to the whiB2 promoter found in M.tuberculosis.
M.smegmatis cells expressing this protein will not grow on plates.
Low level expression in M.smegmatis leads to superinfection
exclusion, possibly via modification to the cell wall.
{ECO:0000269|PubMed:20545868}.
-!- COFACTOR:
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
Evidence={ECO:0000250, ECO:0000305};
Note=Binds 1 [4Fe-4S] cluster per subunit. Contains 1 [2Fe-2S]
cluster after reconstitution of overexpressed protein from E.coli.
Following nitrosylation of the [4Fe-4S] cluster binds 1 [4Fe-
8(NO)] cluster per subunit. {ECO:0000250, ECO:0000305};
-!- SUBUNIT: Forms homodimers in the absence of a reducing agent.
{ECO:0000269|PubMed:20545868}.
-!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
-!- INDUCTION: Expressed in the early phase of the viral replicative
cycle. {ECO:0000269|PubMed:20545868}.
-!- PTM: The iron-sulfur cluster is sensitive to air and oxidizing
agents; degradation is complete in 3 hours upon exposure to air.
-!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
{ECO:0000250}.
-!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
formed. {ECO:0000250}.
-!- DISRUPTION PHENOTYPE: Dispensible for viral growth in M.smegmatis
strain ATCC 700084 / mc(2)155. {ECO:0000269|PubMed:20545868}.
-!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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EMBL; AF068845; AAD17616.1; -; Genomic_DNA.
RefSeq; NP_569784.1; NC_003387.1.
GeneID; 932296; -.
KEGG; vg:932296; -.
KO; K18955; -.
Proteomes; UP000002133; Genome.
GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-KW.
GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW.
HAMAP; MF_01479; WhiB; 1.
InterPro; IPR034768; 4FE4S_WBL.
InterPro; IPR003482; Whib.
PANTHER; PTHR38839; PTHR38839; 1.
Pfam; PF02467; Whib; 1.
PROSITE; PS51674; 4FE4S_WBL; 1.
1: Evidence at protein level;
4Fe-4S; Complete proteome; Disulfide bond; DNA-binding;
Host cytoplasm; Iron; Iron-sulfur; Metal-binding; Reference proteome;
Transcription; Transcription regulation.
CHAIN 1 76 Probable transcriptional regulator
WhiBTM4.
/FTId=PRO_0000420399.
DOMAIN 12 69 4Fe-4S Wbl-type.
METAL 13 13 Dinitrosyliron [4Fe-8(NO)]; alternate.
{ECO:0000250}.
METAL 13 13 Iron-sulfur (4Fe-4S); alternate.
{ECO:0000305}.
METAL 36 36 Dinitrosyliron [4Fe-8(NO)]; alternate.
{ECO:0000250}.
METAL 36 36 Iron-sulfur (4Fe-4S); alternate.
{ECO:0000305}.
METAL 39 39 Dinitrosyliron [4Fe-8(NO)]; alternate.
{ECO:0000250}.
METAL 39 39 Iron-sulfur (4Fe-4S); alternate.
{ECO:0000250}.
METAL 45 45 Dinitrosyliron [4Fe-8(NO)]; alternate.
{ECO:0000250}.
METAL 45 45 Iron-sulfur (4Fe-4S); alternate.
{ECO:0000305}.
MUTAGEN 36 36 C->S: Unstable iron-sulfur center, small
orange colonies in M.smegmatis. Binds
DNA. {ECO:0000269|PubMed:20545868}.
MUTAGEN 39 39 C->F,R: No iron-sulfur cluster, normal
white colonies in M.smegmatis.
{ECO:0000269|PubMed:20545868}.
MUTAGEN 45 45 C->S: Unstable iron-sulfur center, small
orange colonies in M.smegmatis. Binds
DNA. {ECO:0000269|PubMed:20545868}.
MUTAGEN 55 55 L->Q: Small orange colonies in
M.smegmatis. Decreased DNA-binding.
{ECO:0000269|PubMed:20545868}.
MUTAGEN 56 56 V->D: Small orange colonies in
M.smegmatis. No DNA-binding.
{ECO:0000269|PubMed:20545868}.
MUTAGEN 58 58 V->E: Small orange colonies in
M.smegmatis, poor DNA-binding. Decreased
DNA-binding.
{ECO:0000269|PubMed:20545868}.
MUTAGEN 59 59 W->C: Small orange colonies in
M.smegmatis. Decreased DNA-binding.
{ECO:0000269|PubMed:20545868}.
MUTAGEN 67 67 R->C: Small orange colonies in
M.smegmatis. Decreased DNA-binding.
{ECO:0000269|PubMed:20545868}.
SEQUENCE 76 AA; 8491 MW; 3005C683CD4F9352 CRC64;
MHMHMGGDPS AICAQTDPEL WFPDKGQSTR DAKRMCMRCP LLDECRALAL RDPHLVGVWG
GLSAQERRRI RKGASA


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