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Prolactin regulatory element-binding protein (Mammalian guanine nucleotide exchange factor mSec12)

 PREB_MOUSE              Reviewed;         417 AA.
Q9WUQ2; Q9QZ99;
24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
01-NOV-1999, sequence version 1.
12-SEP-2018, entry version 147.
RecName: Full=Prolactin regulatory element-binding protein;
AltName: Full=Mammalian guanine nucleotide exchange factor mSec12;
Name=Preb; Synonyms=Sec12;
Mus musculus (Mouse).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
NCBI_TaxID=10090;
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Lung;
Edgar A.J., Polak J.M.;
"The human and mouse homologues of rat prolactin regulatory element
binding protein.";
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
STRAIN=BALB/cJ; TISSUE=Brain;
PubMed=10704286; DOI=10.1006/geno.1999.6089;
Taylor Clelland C.L., Craciun L., Bancroft C., Lufkin T.;
"Mapping and developmental expression analysis of the WD-repeat gene
Preb.";
Genomics 63:391-399(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=FVB/N; TISSUE=Salivary gland;
PubMed=15489334; DOI=10.1101/gr.2596504;
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA
project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
FUNCTION, AND INTERACTION WITH SAR1B.
PubMed=11422940; DOI=10.1034/j.1600-0854.2001.20704.x;
Weissman J.T., Plutner H., Balch W.E.;
"The mammalian guanine nucleotide exchange factor mSec12 is essential
for activation of the Sar1 GTPase directing endoplasmic reticulum
export.";
Traffic 2:465-475(2001).
[5]
NITRATION [LARGE SCALE ANALYSIS] AT TYR-10, AND IDENTIFICATION BY MASS
SPECTROMETRY [LARGE SCALE ANALYSIS].
TISSUE=Brain;
PubMed=16800626; DOI=10.1021/bi060474w;
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H.,
Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J.,
Squier T.C., Bigelow D.J.;
"Endogenously nitrated proteins in mouse brain: links to
neurodegenerative disease.";
Biochemistry 45:8009-8022(2006).
[6]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
Spleen, and Testis;
PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
"A tissue-specific atlas of mouse protein phosphorylation and
expression.";
Cell 143:1174-1189(2010).
-!- FUNCTION: Guanine nucleotide exchange factor that specifically
activates the small GTPase SAR1B. Mediates the recruitement of
SAR1B and other COPII coat components to endoplasmic reticulum
membranes and is therefore required for the formation of COPII
transport vesicles from the ER. {ECO:0000269|PubMed:11422940}.
-!- FUNCTION: Was first identified based on its probable role in the
regulation of pituitary gene transcription. Binds to the prolactin
gene (PRL) promoter and seems to activate transcription.
{ECO:0000250|UniProtKB:Q9WTV0}.
-!- SUBUNIT: Interacts with SAR1B (GDP-bound form) (PubMed:11422940).
Interacts with CTAGE5; recruits PREB to endoplasmic reticulum exit
sites (By similarity). {ECO:0000250|UniProtKB:Q9HCU5,
ECO:0000269|PubMed:11422940}.
-!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
{ECO:0000250|UniProtKB:Q9WTV0}; Single-pass membrane protein
{ECO:0000250|UniProtKB:Q9WTV0}. Nucleus
{ECO:0000250|UniProtKB:Q9WTV0}. Note=Concentrates at endoplasmic
reticulum exit sites. {ECO:0000250|UniProtKB:Q9HCU5}.
-!- DEVELOPMENTAL STAGE: Detected starting at E10.5 in the ectoderm
and superficial mesoderm surrounding the caudal part of the tail
region. At E12.5 detected in dorsal root ganglia, surface ectoderm
surrounding the caudal part of the tail, the inferior wall of the
genital tubercle, developing liver, in Rathke pouch and in
condensing mesenchyme forming the roof of the skull. At E14.5
detected in the perichondrial region of the craniofacial, axial,
and appendicular skeleton. By E16.5 expression is much lower
throughout the embryo, and is not detectable by E18.5.
{ECO:0000269|PubMed:10704286}.
-----------------------------------------------------------------------
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EMBL; AF150808; AAD31722.1; -; mRNA.
EMBL; AF193017; AAF07954.1; -; mRNA.
EMBL; BC017527; AAH17527.1; -; mRNA.
CCDS; CCDS39051.1; -.
RefSeq; NP_057912.2; NM_016703.3.
UniGene; Mm.272414; -.
ProteinModelPortal; Q9WUQ2; -.
SMR; Q9WUQ2; -.
BioGrid; 206147; 5.
IntAct; Q9WUQ2; 2.
MINT; Q9WUQ2; -.
STRING; 10090.ENSMUSP00000074387; -.
iPTMnet; Q9WUQ2; -.
PhosphoSitePlus; Q9WUQ2; -.
SwissPalm; Q9WUQ2; -.
EPD; Q9WUQ2; -.
MaxQB; Q9WUQ2; -.
PaxDb; Q9WUQ2; -.
PRIDE; Q9WUQ2; -.
Ensembl; ENSMUST00000074840; ENSMUSP00000074387; ENSMUSG00000045302.
GeneID; 50907; -.
KEGG; mmu:50907; -.
UCSC; uc012dui.2; mouse.
CTD; 10113; -.
MGI; MGI:1355326; Preb.
eggNOG; KOG0771; Eukaryota.
eggNOG; ENOG410XRQK; LUCA.
GeneTree; ENSGT00390000018031; -.
HOGENOM; HOG000045302; -.
HOVERGEN; HBG002027; -.
InParanoid; Q9WUQ2; -.
KO; K14003; -.
OMA; AHSMFVT; -.
OrthoDB; EOG091G06TN; -.
PhylomeDB; Q9WUQ2; -.
TreeFam; TF314383; -.
Reactome; R-MMU-204005; COPII-mediated vesicle transport.
Reactome; R-MMU-5694530; Cargo concentration in the ER.
ChiTaRS; Preb; mouse.
PRO; PR:Q9WUQ2; -.
Proteomes; UP000000589; Chromosome 5.
Bgee; ENSMUSG00000045302; Expressed in 285 organ(s), highest expression level in bone fossa.
CleanEx; MM_PREB; -.
ExpressionAtlas; Q9WUQ2; baseline and differential.
Genevisible; Q9WUQ2; MM.
GO; GO:0070971; C:endoplasmic reticulum exit site; ISO:MGI.
GO; GO:0000139; C:Golgi membrane; IEA:GOC.
GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISS:UniProtKB.
GO; GO:0005634; C:nucleus; ISO:MGI.
GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:MGI.
GO; GO:0005096; F:GTPase activator activity; IBA:GO_Central.
GO; GO:0051020; F:GTPase binding; IPI:UniProtKB.
GO; GO:0005090; F:Sar guanyl-nucleotide exchange factor activity; IDA:UniProtKB.
GO; GO:0001077; F:transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding; ISO:MGI.
GO; GO:0048208; P:COPII vesicle coating; ISS:UniProtKB.
GO; GO:0006888; P:ER to Golgi vesicle-mediated transport; ISS:UniProtKB.
GO; GO:0032527; P:protein exit from endoplasmic reticulum; ISS:UniProtKB.
GO; GO:0009306; P:protein secretion; IBA:GO_Central.
GO; GO:0003400; P:regulation of COPII vesicle coating; IBA:GO_Central.
GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:MGI.
InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
InterPro; IPR001680; WD40_repeat.
InterPro; IPR017986; WD40_repeat_dom.
Pfam; PF00400; WD40; 1.
SMART; SM00320; WD40; 3.
SUPFAM; SSF50998; SSF50998; 2.
PROSITE; PS50082; WD_REPEATS_2; 1.
PROSITE; PS50294; WD_REPEATS_REGION; 1.
1: Evidence at protein level;
Activator; Complete proteome; DNA-binding; Endoplasmic reticulum;
ER-Golgi transport; Membrane; Nitration; Nucleus; Protein transport;
Reference proteome; Repeat; Transcription; Transcription regulation;
Transmembrane; Transmembrane helix; Transport; WD repeat.
CHAIN 1 417 Prolactin regulatory element-binding
protein.
/FTId=PRO_0000051155.
TOPO_DOM 1 388 Cytoplasmic. {ECO:0000255}.
TRANSMEM 389 409 Helical. {ECO:0000255}.
TOPO_DOM 410 417 Lumenal. {ECO:0000255}.
REPEAT 152 191 WD 1.
REPEAT 194 232 WD 2.
REPEAT 298 337 WD 3.
MOD_RES 10 10 Nitrated tyrosine.
{ECO:0000244|PubMed:16800626}.
CONFLICT 136 136 Missing (in Ref. 2; AAF07954).
{ECO:0000305}.
SEQUENCE 417 AA; 45437 MW; 89F8B24F9A7684FA CRC64;
MGRRRGVELY RAPFPLYALR IDPKTGLLIA AGGGGAAKTG IKNGVHFLQL ELINGCLSAS
LLHSHDTETR ATMNLALAGD ILAAGQDAQC QLLRFQVHQQ KGSKAEKSGS KEQGPRQRKG
APPAEKKSGA QVHPEGVELK VKNLEAVQTD FSNEPLQKVV CFNHDNTLLA TGGTDGHVRV
WKVPSLEKVL EFKAHEGEIG DLTLGPDGKL VTVGWDFKAS VWQKDQLVTQ LQWQENGPAS
SNTPYRYQAC RFGQVPDQLG GLRLFTVQIP HKRLRQPPPC YLTAWDSSTF LPLRTRSCGH
EVISCLSVSD SGTFLGLGTV TGSVAIYIAF SLQRLYYVKE AHGIVVTDVT FLPEKGCGPK
LLGPHETALF SVAVDSRCQL HLLPSRRSVP VWLLLLLCVG LIIVTILLLQ TAFPGFL


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