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Proline/betaine transporter (Proline porter II) (PPII)

 PROP_ECOLI              Reviewed;         500 AA.
P0C0L7; P30848; Q2M6J3;
08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
08-NOV-2005, sequence version 1.
28-MAR-2018, entry version 110.
RecName: Full=Proline/betaine transporter;
AltName: Full=Proline porter II;
Short=PPII;
Name=proP; OrderedLocusNames=b4111, JW4072;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
PubMed=8421314; DOI=10.1006/jmbi.1993.1030;
Culham D.E., Lasby B., Marangoni A.G., Milner J.L., Steer B.A.,
van Nues R.W., Wood J.M.;
"Isolation and sequencing of Escherichia coli gene proP reveals
unusual structural features of the osmoregulatory proline/betaine
transporter, ProP.";
J. Mol. Biol. 229:268-276(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=7610040; DOI=10.1093/nar/23.12.2105;
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.,
Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the
region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
FUNCTION, AND INDUCTION.
STRAIN=K12;
PubMed=3082857; DOI=10.1128/jb.166.1.331-333.1986;
Gowrishankar J.;
"proP-mediated proline transport also plays a role in Escherichia coli
osmoregulation.";
J. Bacteriol. 166:331-333(1986).
[6]
FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
PubMed=10026245; DOI=10.1021/bi981279n;
Racher K.I., Voegele R.T., Marshall E.V., Culham D.E., Wood J.M.,
Jung H., Bacon M., Cairns M.T., Ferguson S.M., Liang W.J.,
Henderson P.J., White G., Hallett F.R.;
"Purification and reconstitution of an osmosensor: transporter ProP of
Escherichia coli senses and responds to osmotic shifts.";
Biochemistry 38:1676-1684(1999).
[7]
TOPOLOGY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION.
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=15919996; DOI=10.1126/science.1109730;
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane
proteome.";
Science 308:1321-1323(2005).
[8]
STRUCTURE BY NMR OF 464-498.
PubMed=14643666; DOI=10.1016/j.jmb.2003.10.020;
Zoetewey D.L., Tripet B.P., Kutateladze T.G., Overduin M.J.,
Wood J.M., Hodges R.S.;
"Solution structure of the C-terminal antiparallel coiled-coil domain
from Escherichia coli osmosensor ProP.";
J. Mol. Biol. 334:1063-1076(2003).
-!- FUNCTION: Proton symporter that senses osmotic shifts and responds
by importing osmolytes such as proline, glycine betaine,
stachydrine, pipecolic acid, ectoine and taurine. It is both an
osmosensor and an osmoregulator which is available to participate
early in the bacterial osmoregulatory response.
{ECO:0000269|PubMed:10026245, ECO:0000269|PubMed:3082857,
ECO:0000269|PubMed:8421314}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=175 uM for proline {ECO:0000269|PubMed:10026245};
Vmax=83.6 nmol/min/mg enzyme with proline as substrate
{ECO:0000269|PubMed:10026245};
-!- SUBCELLULAR LOCATION: Cell inner membrane
{ECO:0000269|PubMed:10026245, ECO:0000269|PubMed:15919996}; Multi-
pass membrane protein {ECO:0000255}.
-!- INDUCTION: Induced by an increase in the osmolarity of the growth
medium. {ECO:0000269|PubMed:3082857}.
-!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
transporter (TC 2.A.1.1) family. {ECO:0000305}.
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EMBL; M83089; AAB00919.1; -; Genomic_DNA.
EMBL; U14003; AAA97010.1; -; Genomic_DNA.
EMBL; U00096; AAC77072.1; -; Genomic_DNA.
EMBL; AP009048; BAE78113.1; -; Genomic_DNA.
PIR; S32331; S32331.
RefSeq; NP_418535.1; NC_000913.3.
RefSeq; WP_001298520.1; NZ_LN832404.1.
PDB; 1R48; NMR; -; A/B=468-497.
PDB; 1Y8S; Model; -; A=1-500.
PDBsum; 1R48; -.
PDBsum; 1Y8S; -.
ProteinModelPortal; P0C0L7; -.
SMR; P0C0L7; -.
BioGrid; 4263085; 22.
STRING; 316385.ECDH10B_4303; -.
TCDB; 2.A.1.6.4; the major facilitator superfamily (mfs).
PaxDb; P0C0L7; -.
PRIDE; P0C0L7; -.
EnsemblBacteria; AAC77072; AAC77072; b4111.
EnsemblBacteria; BAE78113; BAE78113; BAE78113.
GeneID; 948626; -.
KEGG; ecj:JW4072; -.
KEGG; eco:b4111; -.
PATRIC; fig|1411691.4.peg.2589; -.
EchoBASE; EB1569; -.
EcoGene; EG11612; proP.
eggNOG; ENOG4105CSH; Bacteria.
eggNOG; ENOG410XP7I; LUCA.
HOGENOM; HOG000141611; -.
InParanoid; P0C0L7; -.
KO; K03762; -.
OMA; DKFGRQK; -.
PhylomeDB; P0C0L7; -.
BioCyc; EcoCyc:PROP-MONOMER; -.
BioCyc; MetaCyc:PROP-MONOMER; -.
SABIO-RK; P0C0L7; -.
EvolutionaryTrace; P0C0L7; -.
PRO; PR:P0C0L7; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0016021; C:integral component of membrane; IDA:EcoliWiki.
GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
GO; GO:0005886; C:plasma membrane; IDA:CAFA.
GO; GO:0015653; F:glycine betaine:proton symporter activity; IDA:EcoCyc.
GO; GO:0006865; P:amino acid transport; IDA:EcoliWiki.
GO; GO:0071475; P:cellular hyperosmotic salinity response; IDA:CAFA.
GO; GO:0007231; P:osmosensory signaling pathway; IDA:CAFA.
GO; GO:1905647; P:proline import across plasma membrane; IDA:CAFA.
CDD; cd06174; MFS; 1.
InterPro; IPR020846; MFS_dom.
InterPro; IPR005828; MFS_sugar_transport-like.
InterPro; IPR036259; MFS_trans_sf.
InterPro; IPR004736; MHS_symport.
InterPro; IPR015041; Osmo_CC.
InterPro; IPR036292; ProP_C.
InterPro; IPR005829; Sugar_transporter_CS.
Pfam; PF08946; Osmo_CC; 1.
Pfam; PF00083; Sugar_tr; 1.
SUPFAM; SSF103473; SSF103473; 1.
SUPFAM; SSF103661; SSF103661; 1.
TIGRFAMs; TIGR00883; 2A0106; 1.
PROSITE; PS50850; MFS; 1.
PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
1: Evidence at protein level;
3D-structure; Cell inner membrane; Cell membrane; Coiled coil;
Complete proteome; Membrane; Reference proteome; Symport;
Transmembrane; Transmembrane helix; Transport.
CHAIN 1 500 Proline/betaine transporter.
/FTId=PRO_0000050324.
TOPO_DOM 1 37 Cytoplasmic. {ECO:0000255}.
TRANSMEM 38 58 Helical; Name=1. {ECO:0000255}.
TOPO_DOM 59 65 Periplasmic. {ECO:0000255}.
TRANSMEM 66 86 Helical; Name=2. {ECO:0000255}.
TOPO_DOM 87 97 Cytoplasmic. {ECO:0000255}.
TRANSMEM 98 118 Helical; Name=3. {ECO:0000255}.
TOPO_DOM 119 121 Periplasmic. {ECO:0000255}.
TRANSMEM 122 142 Helical; Name=4. {ECO:0000255}.
TOPO_DOM 143 169 Cytoplasmic. {ECO:0000255}.
TRANSMEM 170 190 Helical; Name=5. {ECO:0000255}.
TOPO_DOM 191 194 Periplasmic. {ECO:0000255}.
TRANSMEM 195 215 Helical; Name=6. {ECO:0000255}.
TOPO_DOM 216 260 Cytoplasmic. {ECO:0000255}.
TRANSMEM 261 281 Helical; Name=7. {ECO:0000255}.
TOPO_DOM 282 297 Periplasmic. {ECO:0000255}.
TRANSMEM 298 318 Helical; Name=8. {ECO:0000255}.
TOPO_DOM 319 325 Cytoplasmic. {ECO:0000255}.
TRANSMEM 326 346 Helical; Name=9. {ECO:0000255}.
TOPO_DOM 347 350 Periplasmic. {ECO:0000255}.
TRANSMEM 351 371 Helical; Name=10. {ECO:0000255}.
TOPO_DOM 372 390 Cytoplasmic. {ECO:0000255}.
TRANSMEM 391 411 Helical; Name=11. {ECO:0000255}.
TOPO_DOM 412 416 Periplasmic. {ECO:0000255}.
TRANSMEM 417 437 Helical; Name=12. {ECO:0000255}.
TOPO_DOM 438 500 Cytoplasmic.
{ECO:0000269|PubMed:15919996}.
COILED 453 498 {ECO:0000255}.
HELIX 469 494 {ECO:0000244|PDB:1R48}.
SEQUENCE 500 AA; 54846 MW; 60EDDBF61190DDFC CRC64;
MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD
PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD
TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA
GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE
GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM
LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP
AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE
IADLQAKRTR LVQQHPRIDE


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