Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Protein CWH43 (Calcofluor white hypersensitive protein)

 CWH43_YEAST             Reviewed;         953 AA.
P25618; D6VR25; Q8NIM1;
01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
24-OCT-2003, sequence version 2.
12-SEP-2018, entry version 136.
RecName: Full=Protein CWH43;
AltName: Full=Calcofluor white hypersensitive protein;
Name=CWH43; OrderedLocusNames=YCR017C; ORFNames=YCR17C;
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
NCBI_TaxID=559292;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=1574125; DOI=10.1038/357038a0;
Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M.,
Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W.,
Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H.,
Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V.,
Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A.,
de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H.,
Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K.,
Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B.,
Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A.,
Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y.,
Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R.,
Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G.,
Tzermia M., Urrestarazu L.A., Valle G., Vetter I.,
van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H.,
Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C.,
Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G.;
"The complete DNA sequence of yeast chromosome III.";
Nature 357:38-46(1992).
[2]
SEQUENCE REVISION TO 229; 329; 634 AND 873.
Valles G., Volckaerts G.;
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
[3]
GENOME REANNOTATION.
STRAIN=ATCC 204508 / S288c;
PubMed=24374639; DOI=10.1534/g3.113.008995;
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
Cherry J.M.;
"The reference genome sequence of Saccharomyces cerevisiae: Then and
now.";
G3 (Bethesda) 4:389-398(2014).
[4]
FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-57.
PubMed=11427965; DOI=10.1002/yea.731;
Martin-Yken H., Dagkessamanskaia A., De Groot P., Ram A., Klis F.,
Francois J.;
"Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative
sensor/transporter protein upstream of the BCK2 branch of the PKC1-
dependent cell wall integrity pathway.";
Yeast 18:827-840(2001).
[5]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=ATCC 208353 / W303-1A;
PubMed=16847258; DOI=10.1073/pnas.0604075103;
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane
proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
[6]
FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AND MUTAGENESIS OF GLY-57;
HIS-472; ASP-693; HIS-770; HIS-771; GLU-807; ASP-862 AND ARG-882.
PubMed=17761529; DOI=10.1091/mbc.E07-05-0482;
Umemura M., Fujita M., Yoko-O T., Fukamizu A., Jigami Y.;
"Saccharomyces cerevisiae CWH43 is involved in the remodeling of the
lipid moiety of GPI anchors to ceramides.";
Mol. Biol. Cell 18:4304-4316(2007).
[7]
FUNCTION, AND MUTAGENESIS OF ASP-713 AND HIS-802.
PubMed=17714445; DOI=10.1111/j.1365-2958.2007.05883.x;
Ghugtyal V., Vionnet C., Roubaty C., Conzelmann A.;
"CWH43 is required for the introduction of ceramides into GPI anchors
in Saccharomyces cerevisiae.";
Mol. Microbiol. 65:1493-1502(2007).
[8]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth
phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
-!- FUNCTION: Involved in the maintenance of cell wall integrity.
Required for the replacement of the diacylglycerol moiety by
ceramides during GPI-anchor maturation.
{ECO:0000269|PubMed:11427965, ECO:0000269|PubMed:17714445,
ECO:0000269|PubMed:17761529}.
-!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Note=Concentrates to the bud tip of small budded cells and to the
neck of dividing cells.
-!- DOMAIN: The PGAP2-like region interacts with the PGAP2IP-like
region. {ECO:0000269|PubMed:17761529}.
-!- SIMILARITY: In the N-terminal section; belongs to the PGAP2
family. {ECO:0000305}.
-!- SIMILARITY: In the C-terminal section; belongs to the PGAP2IP
family. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; X59720; CAC42972.1; -; Genomic_DNA.
EMBL; BK006937; DAA07494.1; -; Genomic_DNA.
PIR; S19427; S19427.
RefSeq; NP_009943.2; NM_001178730.1.
ProteinModelPortal; P25618; -.
BioGrid; 30996; 174.
STRING; 4932.YCR017C; -.
TCDB; 9.B.131.1.1; the post-gpi attachment protein (p-gap2) family.
iPTMnet; P25618; -.
MaxQB; P25618; -.
PaxDb; P25618; -.
PRIDE; P25618; -.
EnsemblFungi; CAC42972; CAC42972; CAC42972.
EnsemblFungi; YCR017C; YCR017C; YCR017C.
GeneID; 850376; -.
KEGG; sce:YCR017C; -.
EuPathDB; FungiDB:YCR017C; -.
SGD; S000000610; CWH43.
GeneTree; ENSGT00510000048509; -.
HOGENOM; HOG000196377; -.
InParanoid; P25618; -.
OMA; WYTSLNP; -.
OrthoDB; EOG092C05MJ; -.
BioCyc; YEAST:G3O-29332-MONOMER; -.
PRO; PR:P25618; -.
Proteomes; UP000002311; Chromosome III.
GO; GO:0005935; C:cellular bud neck; IDA:UniProtKB.
GO; GO:0005934; C:cellular bud tip; IDA:UniProtKB.
GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
GO; GO:0071555; P:cell wall organization; IGI:UniProtKB.
GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
GO; GO:0006506; P:GPI anchor biosynthetic process; IMP:SGD.
GO; GO:0006505; P:GPI anchor metabolic process; IMP:SGD.
Gene3D; 3.60.10.10; -; 1.
InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
InterPro; IPR019402; Frag1/DRAM/Sfk1.
InterPro; IPR027317; PGAP2IP.
PANTHER; PTHR14859:SF1; PTHR14859:SF1; 1.
Pfam; PF10277; Frag1; 1.
SUPFAM; SSF56219; SSF56219; 1.
1: Evidence at protein level;
Cell membrane; Complete proteome; Endoplasmic reticulum; Glycoprotein;
GPI-anchor biosynthesis; Membrane; Reference proteome; Transmembrane;
Transmembrane helix.
CHAIN 1 953 Protein CWH43.
/FTId=PRO_0000021052.
TOPO_DOM 1 7 Cytoplasmic. {ECO:0000255}.
TOPO_DOM 29 64 Extracellular. {ECO:0000255}.
TRANSMEM 65 85 Helical. {ECO:0000255}.
TOPO_DOM 86 91 Cytoplasmic. {ECO:0000255}.
TRANSMEM 92 112 Helical. {ECO:0000255}.
TOPO_DOM 113 117 Extracellular. {ECO:0000255}.
TRANSMEM 118 138 Helical. {ECO:0000255}.
TOPO_DOM 139 149 Cytoplasmic. {ECO:0000255}.
TRANSMEM 150 170 Helical. {ECO:0000255}.
TOPO_DOM 171 175 Extracellular. {ECO:0000255}.
TRANSMEM 176 196 Helical. {ECO:0000255}.
TOPO_DOM 197 278 Cytoplasmic. {ECO:0000255}.
TRANSMEM 279 299 Helical. {ECO:0000255}.
TOPO_DOM 300 307 Extracellular. {ECO:0000255}.
TRANSMEM 308 328 Helical. {ECO:0000255}.
TOPO_DOM 329 330 Cytoplasmic. {ECO:0000255}.
TRANSMEM 331 351 Helical. {ECO:0000255}.
TOPO_DOM 352 352 Extracellular. {ECO:0000255}.
TRANSMEM 353 368 Helical. {ECO:0000255}.
TOPO_DOM 369 380 Cytoplasmic. {ECO:0000255}.
TRANSMEM 381 401 Helical. {ECO:0000255}.
TOPO_DOM 402 420 Extracellular. {ECO:0000255}.
TRANSMEM 421 441 Helical. {ECO:0000255}.
TOPO_DOM 442 450 Cytoplasmic. {ECO:0000255}.
TRANSMEM 451 471 Helical. {ECO:0000255}.
TOPO_DOM 472 495 Extracellular. {ECO:0000255}.
TRANSMEM 496 516 Helical. {ECO:0000255}.
TOPO_DOM 517 517 Cytoplasmic. {ECO:0000255}.
TRANSMEM 518 538 Helical. {ECO:0000255}.
TOPO_DOM 539 547 Extracellular. {ECO:0000255}.
TRANSMEM 548 568 Helical. {ECO:0000255}.
TOPO_DOM 569 585 Cytoplasmic. {ECO:0000255}.
TRANSMEM 586 606 Helical. {ECO:0000255}.
TOPO_DOM 607 613 Extracellular. {ECO:0000255}.
TRANSMEM 614 634 Helical. {ECO:0000255}.
TOPO_DOM 635 642 Cytoplasmic. {ECO:0000255}.
TRANSMEM 643 663 Helical. {ECO:0000255}.
TOPO_DOM 664 953 Extracellular. {ECO:0000255}.
REGION 1 229 PGAP2-like.
REGION 230 953 PGAP2IP-like.
REGION 862 882 Required for function in lipid
remodeling.
ACT_SITE 802 802 {ECO:0000305}.
CARBOHYD 419 419 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 490 490 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 767 767 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 792 792 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
CARBOHYD 825 825 N-linked (GlcNAc...) asparagine.
{ECO:0000255}.
MUTAGEN 57 57 G->R: Causes destabilization of the
protein and induces the release of cell
wall proteins in the culture medium.
{ECO:0000269|PubMed:11427965,
ECO:0000269|PubMed:17761529}.
MUTAGEN 472 472 H->A: No effect on introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 693 693 D->A: No effect on introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 713 713 D->A: Impairs the introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17714445}.
MUTAGEN 770 770 H->A: No effect on introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 771 771 H->A: No effect on introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 802 802 H->A: Abrogates the introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17714445}.
MUTAGEN 807 807 E->A: No effect on introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 862 862 D->A: Impairs the introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
MUTAGEN 882 882 R->A: Abrogates the introduction of
ceramides into the GPI anchor.
{ECO:0000269|PubMed:17761529}.
SEQUENCE 953 AA; 107883 MW; 9F56CCD85824E848 CRC64;
MLIINGKIIP IAHTICAFSA FFAALVTGYS LHFHKIVTNA HYTYPDEWFP SVSATIGDRY
PERSIFQILI ALTAFPRFLL LLGHYYLNQS KVCFLVGVLR TVSCGGWVYI TSTDDHDIHD
IFMITYIVLT LPWDIMITRY SSPLTSKNKG LTATIFFGTL FPMIYWYIQH SVQQRAGAYS
IYAYFEWSLI LLDIAFDAFA YADFKKIDIV LAFNEKPGNT SFFQIRDSSP INYGEEKSSE
LQKSGEKKVE KEKPVARSAT GSYFRFDSFF YLLTNIFNGF LFWSNVTSLL CSIWHFPLWY
MGISGYEAAI LGYLGPIFLY LPFVSEAFTQ YGVLLGGIIA IGAYIVQMPE LRLISVAVGT
SITVATFVQN LRYITNAETS FSFALTWLLG LVASVILKMG FYTNNPTWVI LDERNGGYNK
TALVLTVLFG MLSPYVNSIN FEGKRNAQAK SASLIGKLFL AVGFGSLLFG IHQLLTDSST
TIYWAWEGYN ESHGPLPWPW GALTCTVMLF ASLSSVKFMG KPLVPCLLLL ISTAVLSARS
ITQWPKYIFG GLLYAIAMLW LVPSYFSALG QVQNIWVYVL SFSVYIIFVL AHVWVVAYAF
VPMGWVLREK IETVLAFSST FIIIGALTCK NLNIQLVTMG KKFFIYVFFF AVALLSLTAR
FVYDIRPTGI PQPYHPDSQL ITAGIWTIHF GLDNDMWASE DRMINLIKDM ELDVVGLLET
DTQRITMGNR DLTSKLAHDL NMYADFGPGP NKHTWGCVLL SKFPIVNSTH HLLPSPVGEL
APAIHATLQT YNDTLVDVFV FHSGQEEDEE DRRLQSNYMA KLMGNTTRPA ILLSYLVVDP
GEGNYNTYVS ETSGMHDIDP SDDDRWCEYI LYRGLRRTGY ARVARGTITD TELQVGKFQV
LSEQALVEHS DSMYEYGHMS EPEYEDMKFP DKFLGEGERG HFYHVFDEPR YYL


Related products :

Catalog number Product name Quantity
EIAAB30671 Cell wall biogenesis protein 43 C-terminal homolog,Cwh43,Mouse,Mus musculus,PGAP2-interacting protein,Pgap2ip
EIAAB30670 Cell wall biogenesis protein 43 C-terminal homolog,CWH43,Homo sapiens,Human,PGAP2-interacting protein,PGAP2IP
PG2IP_MOUSE ELISA Kit FOR PGAP2-interacting protein; organism: Mouse; gene name: Cwh43 96T
orb90500 Hypersensitive WB Chemiluminescent Substrate Hypersensitive WB Chemiluminescent Substrate For research use only. 5 ml (20x)
orb81138 Ganciclovir protein Ganciclovir is white to off-white crystalline powder with molecular formula of C9H13N504 and molecular weight of 255.23. For research use only. 100 mg
V-09398 VASELINE WHITE (Paraffin Soft White, Petroleum Jelly White ) CAS: 8009-03-8 500 gm
V-09398 VASELINE WHITE (Paraffin Soft White, Petroleum Jelly White ) CAS: 8009-03-8 5 kg
P-06710 PETROLEUM JELLY WHITE (Paraffin Soft white) (Vaseline White) CAS: 8009-03-8 500 gm
P-06710 PETROLEUM JELLY WHITE (Paraffin Soft white) (Vaseline White) CAS: 8009-03-8 5 kg
orb80938 Modified Affinity Purified Avidin protein Avidin is basic charged glycoprotein present in the egg white and in some extent in tissues of various animals. Avidin is homotetrameric protein that contains 2 mg
102-90 Avidin, Egg White, >< per u>10 U per mg Protein 500 mg
102-90 Avidin, Egg White, >< per u>10 U per mg Protein 250 mg
102-90 Avidin, Egg White, >< per u>10 U per mg Protein 25 mg
102-90 Avidin, Egg White, >< per u>10 U per mg Protein 5 mg
028987A CWH43 250ul
035863A Cwh43 250ul
051841A Cwh43 250ul
CR0001-25 Hypersensitive WB Chemiluminescent Substrate 25ml (20
BWR1064 Hypersensitive ECL Chemiluminescent Substrate (RTU) 100 ml
CR0001-5 Hypersensitive WB Chemiluminescent Substrate 5ml (20
CR0001-10 Hypersensitive WB Chemiluminescent Substrate 10ml (20
CR0001-25 Hypersensitive WB Chemiluminescent Substrate 25ml (20
CR0001-5 Hypersensitive WB Chemiluminescent Substrate 5ml (20
CR0001-10 Hypersensitive WB Chemiluminescent Substrate 10ml (20
BWR1064 Hypersensitive ECL Chemiluminescent Substrate (RTU) 100 ml


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur