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Protein single-minded

 SIM_DROME               Reviewed;         697 AA.
P05709; O96521; Q7KSL7; Q8MQI7; Q9VFZ3;
01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
23-MAY-2003, sequence version 3.
07-NOV-2018, entry version 185.
RecName: Full=Protein single-minded;
Name=sim; ORFNames=CG7771;
Drosophila melanogaster (Fruit fly).
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha;
Ephydroidea; Drosophilidae; Drosophila; Sophophora.
NCBI_TaxID=7227;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B), AND FUNCTION.
PubMed=9840810;
Kasai Y., Stahl S., Crews S.;
"Specification of the Drosophila CNS midline cell lineage: direct
control of single-minded transcription by dorsal/ventral patterning
genes.";
Gene Expr. 7:171-189(1998).
[2]
NUCLEOTIDE SEQUENCE (ALLELE SIM-J1-47), FUNCTION, TISSUE SPECIFICITY,
AND MUTAGENESIS OF SER-65.
PubMed=12221007; DOI=10.1006/dbio.2002.0770;
Pielage J., Steffes G., Lau D.C., Parente B.A., Crews S.T.,
Strauss R., Klambt C.;
"Novel behavioral and developmental defects associated with Drosophila
single-minded.";
Dev. Biol. 249:283-299(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
PubMed=10731132; DOI=10.1126/science.287.5461.2185;
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[4]
GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
STRAIN=Berkeley;
PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a
systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
STRAIN=Berkeley; TISSUE=Embryo;
PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[6]
NUCLEOTIDE SEQUENCE OF 25-42, AND SIMILARITY TO HLH PROTEINS.
PubMed=1760843; DOI=10.1016/0092-8674(91)90292-7;
Nambu J.R., Lewis J.O., Wharton K.A. Jr., Crews S.T.;
"The Drosophila single-minded gene encodes a helix-loop-helix protein
that acts as a master regulator of CNS midline development.";
Cell 67:1157-1167(1991).
[7]
NUCLEOTIDE SEQUENCE [MRNA] OF 43-697.
PubMed=3345560; DOI=10.1016/0092-8674(88)90538-7;
Crews S.T., Thomas J.B., Goodman C.S.;
"The Drosophila single-minded gene encodes a nuclear protein with
sequence similarity to the per gene product.";
Cell 52:143-151(1988).
[8]
FUNCTION IN SYNAPSE DEVELOPMENT.
PubMed=23644463; DOI=10.1136/jmedgenet-2012-101490;
Willemsen M.H., Nijhof B., Fenckova M., Nillesen W.M., Bongers E.M.,
Castells-Nobau A., Asztalos L., Viragh E., van Bon B.W., Tezel E.,
Veltman J.A., Brunner H.G., de Vries B.B., de Ligt J., Yntema H.G.,
van Bokhoven H., Isidor B., Le Caignec C., Lorino E., Asztalos Z.,
Koolen D.A., Vissers L.E., Schenck A., Kleefstra T.;
"GATAD2B loss-of-function mutations cause a recognisable syndrome with
intellectual disability and are associated with learning deficits and
synaptic undergrowth in Drosophila.";
J. Med. Genet. 50:507-514(2013).
-!- FUNCTION: Transcription factor that functions as a master
developmental regulator controlling midline development of the
ventral nerve cord. Required to correctly specify the formation of
the central brain complex, which controls walking behavior. Also
required for correct patterning of the embryonic genital disk and
anal pad anlage. Plays a role in synapse development.
{ECO:0000269|PubMed:12221007, ECO:0000269|PubMed:23644463,
ECO:0000269|PubMed:9840810}.
-!- SUBUNIT: Efficient DNA binding requires dimerization with another
bHLH protein.
-!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-
ProRule:PRU00981}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=A;
IsoId=P05709-1; Sequence=Displayed;
Note=No experimental confirmation available.;
Name=B;
IsoId=P05709-2; Sequence=VSP_011812;
-!- TISSUE SPECIFICITY: Embryonic nerve cord.
{ECO:0000269|PubMed:12221007}.
-!- POLYMORPHISM: Berkeley strain has 11 A-A-Q repeats.
{ECO:0000269|PubMed:1760843}.
-!- MISCELLANEOUS: Mutations result in the loss of the precursor cells
that give rise to midline cells of the embryonic central nervous
system.
-!- SEQUENCE CAUTION:
Sequence=AAC64519.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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EMBL; AF071934; AAC64519.1; ALT_SEQ; Genomic_DNA.
EMBL; AE014297; AAF54902.3; -; Genomic_DNA.
EMBL; AE014297; AAN14343.3; -; Genomic_DNA.
EMBL; AY129457; AAM76199.1; -; mRNA.
EMBL; M19020; AAA28900.1; -; mRNA.
PIR; A29945; A29945.
PIR; A41647; A41647.
RefSeq; NP_524340.2; NM_079616.4.
RefSeq; NP_731771.3; NM_169495.4.
UniGene; Dm.4557; -.
ProteinModelPortal; P05709; -.
SMR; P05709; -.
BioGrid; 66699; 8.
IntAct; P05709; 1.
STRING; 7227.FBpp0082178; -.
PaxDb; P05709; -.
PRIDE; P05709; -.
GeneID; 41612; -.
KEGG; dme:Dmel_CG7771; -.
CTD; 41612; -.
FlyBase; FBgn0004666; sim.
eggNOG; KOG3559; Eukaryota.
eggNOG; ENOG410XY57; LUCA.
InParanoid; P05709; -.
KO; K09100; -.
OrthoDB; EOG091G06FQ; -.
PhylomeDB; P05709; -.
GenomeRNAi; 41612; -.
PRO; PR:P05709; -.
Proteomes; UP000000803; Chromosome 3R.
Bgee; FBgn0004666; Expressed in 23 organ(s), highest expression level in oviduct (Drosophila).
ExpressionAtlas; P05709; baseline and differential.
Genevisible; P05709; DM.
GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
GO; GO:0005634; C:nucleus; IDA:UniProtKB.
GO; GO:0005667; C:transcription factor complex; IEA:InterPro.
GO; GO:0003700; F:DNA-binding transcription factor activity; IPI:FlyBase.
GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
GO; GO:0000978; F:RNA polymerase II proximal promoter sequence-specific DNA binding; IDA:FlyBase.
GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
GO; GO:0000982; F:transcription factor activity, RNA polymerase II proximal promoter sequence-specific DNA binding; IDA:FlyBase.
GO; GO:0007628; P:adult walking behavior; IMP:FlyBase.
GO; GO:0007411; P:axon guidance; IMP:FlyBase.
GO; GO:0007409; P:axonogenesis; IMP:FlyBase.
GO; GO:0007420; P:brain development; IMP:FlyBase.
GO; GO:0035225; P:determination of genital disc primordium; IMP:FlyBase.
GO; GO:0007398; P:ectoderm development; TAS:FlyBase.
GO; GO:0040011; P:locomotion; TAS:FlyBase.
GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
GO; GO:0006355; P:regulation of transcription, DNA-templated; NAS:UniProtKB.
GO; GO:0007419; P:ventral cord development; IMP:FlyBase.
GO; GO:0007418; P:ventral midline development; IMP:UniProtKB.
CDD; cd00083; HLH; 1.
CDD; cd00130; PAS; 2.
Gene3D; 4.10.280.10; -; 1.
InterPro; IPR011598; bHLH_dom.
InterPro; IPR036638; HLH_DNA-bd_sf.
InterPro; IPR001067; Nuc_translocat.
InterPro; IPR001610; PAC.
InterPro; IPR000014; PAS.
InterPro; IPR035965; PAS-like_dom_sf.
InterPro; IPR013767; PAS_fold.
InterPro; IPR013655; PAS_fold_3.
Pfam; PF00010; HLH; 1.
Pfam; PF00989; PAS; 1.
Pfam; PF08447; PAS_3; 1.
PRINTS; PR00785; NCTRNSLOCATR.
SMART; SM00353; HLH; 1.
SMART; SM00086; PAC; 1.
SMART; SM00091; PAS; 2.
SUPFAM; SSF47459; SSF47459; 1.
SUPFAM; SSF55785; SSF55785; 2.
TIGRFAMs; TIGR00229; sensory_box; 1.
PROSITE; PS50888; BHLH; 1.
PROSITE; PS50112; PAS; 2.
1: Evidence at protein level;
Alternative splicing; Complete proteome; Developmental protein;
Differentiation; DNA-binding; Neurogenesis; Nucleus; Polymorphism;
Reference proteome; Repeat; Transcription; Transcription regulation.
CHAIN 1 697 Protein single-minded.
/FTId=PRO_0000127438.
DOMAIN 24 77 bHLH. {ECO:0000255|PROSITE-
ProRule:PRU00981}.
DOMAIN 100 172 PAS 1. {ECO:0000255|PROSITE-
ProRule:PRU00140}.
DOMAIN 266 336 PAS 2. {ECO:0000255|PROSITE-
ProRule:PRU00140}.
REGION 406 446 14 X 3 AA repeats of A-A-Q (approximate).
COMPBIAS 553 672 Ser-rich.
COMPBIAS 673 693 Gln/His-rich.
VAR_SEQ 1 24 Missing (in isoform B). {ECO:0000305}.
/FTId=VSP_011812.
VARIANT 406 414 Missing (in strain: Berkeley).
MUTAGEN 65 65 S->F: In allele sim-J1-47; temperature
sensitive embryonic midline axon
phenotype. {ECO:0000269|PubMed:12221007}.
CONFLICT 151 151 I -> Y (in Ref. 1; AAC64519).
{ECO:0000305}.
SEQUENCE 697 AA; 76475 MW; 588414A4A17101AD CRC64;
MTNHRRVRKD CYESRLHDIA KTCAMKEKSK NAARTRREKE NTEFCELAKL LPLPAAITSQ
LDKASVIRLT TSYLKMRQVF PDGLGEAWGS SPAMQRGATI KELGSHLLQT LDGFIFVVAP
DGKIMYISET ASVHLGLSQV ELTGNSIFEY IHNYDQDEMN AILSLHPHIN QHPLAQTHTP
IGSPNGVQHP SAYDHDRGSH TIEIEKTFFL RMKCVLAKRN AGLTTSGFKV IHCSGYLKAR
IYPDRGDGQG SLIQNLGLVA VGHSLPSSAI TEIKLHQNMF MFRAKLDMKL IFFDARVSQL
TGYEPQDLIE KTLYQYIHAA DIMAMRCSHQ ILLYKGQVTT KYYRFLTKGG GWVWVQSYAT
LVHNSRSSRE VFIVSVNYVL SEREVKDLVL NEIQTGVVKR EPISPAAQAA QAAQAAQAAQ
AAQAAQAAQA AQAAQAAHVA QAVQAQVVVV PQQSVVVQPQ CAGATGQPVG PGTPVSLALS
ASPKLDPYFE PELPLQPAVT PVPPTNNSSS SSNNNNGVWH HHHVQQQQQS GSMDHDSLSY
TQLYPPLNDL VVSSSSSVGG GTASSAGGGS SASASSSGVY STEMQYPDTT TGNLYYNNNN
HYYYDYDATV DVATSMIRPF SANSNSCSSS SESERQLSTG NASIVNETSP SQTTYSDLSH
NFELSYFSDN SSQQHQHQQQ QQHLMEQQHL QYQYATW


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