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Purine ribonucleoside efflux pump NepI

 NEPI_ECOLI              Reviewed;         396 AA.
P0ADL1; P31438; P76725; Q2M7X5; Q5I3I1;
06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
10-JUL-2007, sequence version 2.
28-MAR-2018, entry version 96.
RecName: Full=Purine ribonucleoside efflux pump NepI;
Name=nepI; Synonyms=yicM; OrderedLocusNames=b3662, JW5938;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=7686882; DOI=10.1006/geno.1993.1230;
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
"DNA sequence and analysis of 136 kilobases of the Escherichia coli
genome: organizational symmetry around the origin of replication.";
Genomics 16:551-561(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 158-396.
STRAIN=BEN2908 / O2:K1:H5 / APEC;
PubMed=16428402; DOI=10.1128/JB.188.3.977-987.2006;
Chouikha I., Germon P., Bree A., Gilot P., Moulin-Schouleur M.,
Schouler C.;
"A selC-associated genomic island of the extraintestinal avian
pathogenic Escherichia coli strain BEN2908 is involved in carbohydrate
uptake and virulence.";
J. Bacteriol. 188:977-987(2006).
[5]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=15919996; DOI=10.1126/science.1109730;
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane
proteome.";
Science 308:1321-1323(2005).
[6]
FUNCTION, INDUCTION, AND CHARACTERIZATION OF TRANSLATIONAL START SITE.
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=16040204; DOI=10.1016/j.femsle.2005.06.051;
Gronskiy S.V., Zakataeva N.P., Vitushkina M.V., Ptitsyn L.R.,
Altman I.B., Novikova A.E., Livshits V.A.;
"The yicM (nepI) gene of Escherichia coli encodes a major facilitator
superfamily protein involved in efflux of purine ribonucleosides.";
FEMS Microbiol. Lett. 250:39-47(2005).
-!- FUNCTION: Involved in the efflux of purine ribonucleosides, such
as guanosine, adenosine and especially inosine. Involved in the
resistance to 6-mercaptopurine. {ECO:0000269|PubMed:16040204}.
-!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
protein.
-!- INDUCTION: In stationary phase. {ECO:0000269|PubMed:16040204}.
-!- SIMILARITY: Belongs to the major facilitator superfamily. DHA1
family. NepI (TC 2.A.1.2.26) subfamily. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA62014.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Sequence=AAW51767.1; Type=Frameshift; Positions=367; Evidence={ECO:0000305};
Sequence=BAE77631.1; Type=Erroneous initiation; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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EMBL; L10328; AAA62014.1; ALT_INIT; Genomic_DNA.
EMBL; U00096; AAC76685.2; -; Genomic_DNA.
EMBL; AP009048; BAE77631.1; ALT_INIT; Genomic_DNA.
EMBL; AY857617; AAW51767.1; ALT_FRAME; Genomic_DNA.
PIR; G65167; G65167.
RefSeq; NP_418118.2; NC_000913.3.
RefSeq; WP_001288549.1; NZ_LN832404.1.
ProteinModelPortal; P0ADL1; -.
BioGrid; 4261472; 10.
DIP; DIP-48180N; -.
STRING; 316385.ECDH10B_3845; -.
TCDB; 2.A.1.2.26; the major facilitator superfamily (mfs).
PaxDb; P0ADL1; -.
PRIDE; P0ADL1; -.
EnsemblBacteria; AAC76685; AAC76685; b3662.
EnsemblBacteria; BAE77631; BAE77631; BAE77631.
GeneID; 948213; -.
KEGG; ecj:JW5938; -.
KEGG; eco:b3662; -.
PATRIC; fig|511145.12.peg.3784; -.
EchoBASE; EB1640; -.
EcoGene; EG11689; yicM.
eggNOG; ENOG4107RTG; Bacteria.
eggNOG; COG2814; LUCA.
HOGENOM; HOG000136928; -.
InParanoid; P0ADL1; -.
KO; K03445; -.
OMA; LIAAEFM; -.
PhylomeDB; P0ADL1; -.
BioCyc; EcoCyc:YICM-MONOMER; -.
BioCyc; MetaCyc:YICM-MONOMER; -.
PRO; PR:P0ADL1; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
GO; GO:0015211; F:purine nucleoside transmembrane transporter activity; IMP:EcoCyc.
GO; GO:0015860; P:purine nucleoside transmembrane transport; IMP:EcoCyc.
CDD; cd06174; MFS; 1.
HAMAP; MF_01189; MFS_NepI; 1.
InterPro; IPR011701; MFS.
InterPro; IPR020846; MFS_dom.
InterPro; IPR023680; MFS_NepI.
InterPro; IPR036259; MFS_trans_sf.
Pfam; PF07690; MFS_1; 1.
SUPFAM; SSF103473; SSF103473; 1.
PROSITE; PS50850; MFS; 1.
1: Evidence at protein level;
Cell inner membrane; Cell membrane; Complete proteome; Membrane;
Reference proteome; Transmembrane; Transmembrane helix; Transport.
CHAIN 1 396 Purine ribonucleoside efflux pump NepI.
/FTId=PRO_0000169616.
TOPO_DOM 1 21 Cytoplasmic. {ECO:0000255}.
TRANSMEM 22 42 Helical. {ECO:0000255}.
TOPO_DOM 43 54 Periplasmic. {ECO:0000255}.
TRANSMEM 55 75 Helical. {ECO:0000255}.
TOPO_DOM 76 85 Cytoplasmic. {ECO:0000255}.
TRANSMEM 86 106 Helical. {ECO:0000255}.
TOPO_DOM 107 107 Periplasmic. {ECO:0000255}.
TRANSMEM 108 128 Helical. {ECO:0000255}.
TOPO_DOM 129 147 Cytoplasmic. {ECO:0000255}.
TRANSMEM 148 168 Helical. {ECO:0000255}.
TOPO_DOM 169 175 Periplasmic. {ECO:0000255}.
TRANSMEM 176 196 Helical. {ECO:0000255}.
TOPO_DOM 197 215 Cytoplasmic. {ECO:0000255}.
TRANSMEM 216 236 Helical. {ECO:0000255}.
TOPO_DOM 237 255 Periplasmic. {ECO:0000255}.
TRANSMEM 256 276 Helical. {ECO:0000255}.
TOPO_DOM 277 281 Cytoplasmic. {ECO:0000255}.
TRANSMEM 282 302 Helical. {ECO:0000255}.
TOPO_DOM 303 305 Periplasmic. {ECO:0000255}.
TRANSMEM 306 326 Helical. {ECO:0000255}.
TOPO_DOM 327 343 Cytoplasmic. {ECO:0000255}.
TRANSMEM 344 364 Helical. {ECO:0000255}.
TOPO_DOM 365 366 Periplasmic. {ECO:0000255}.
TRANSMEM 367 387 Helical. {ECO:0000255}.
TOPO_DOM 388 396 Cytoplasmic. {ECO:0000255}.
VARIANT 158 158 V -> M (in strain: BEN2908 / O2:K1:H5 /
APEC).
VARIANT 182 182 V -> A (in strain: BEN2908 / O2:K1:H5 /
APEC).
VARIANT 267 267 I -> V (in strain: BEN2908 / O2:K1:H5 /
APEC).
VARIANT 289 290 LI -> FV (in strain: BEN2908 / O2:K1:H5 /
APEC).
VARIANT 293 293 V -> L (in strain: BEN2908 / O2:K1:H5 /
APEC).
VARIANT 322 322 V -> I (in strain: BEN2908 / O2:K1:H5 /
APEC).
SEQUENCE 396 AA; 41842 MW; DB240A4A8C1B9D1B CRC64;
MSEFIAENRG ADAITRPNWS AVFSVAFCVA CLIIVEFLPV SLLTPMAQDL GISEGVAGQS
VTVTAFVAMF ASLFITQTIQ ATDRRYVVIL FAVLLTLSCL LVSFANSFSL LLIGRACLGL
ALGGFWAMSA SLTMRLVPPR TVPKALSVIF GAVSIALVIA APLGSFLGEL IGWRNVFNAA
AVMGVLCIFW IIKSLPSLPG EPSHQKQNTF RLLQRPGVMA GMIAIFMSFA GQFAFFTYIR
PVYMNLAGFG VDGLTLVLLS FGIASFIGTS LSSFILKRSV KLALAGAPLI LAVSALVLTL
WGSDKIVATG VAIIWGLTFA LVPVGWSTWI TRSLADQAEK AGSIQVAVIQ LANTCGAAIG
GYALDNIGLT SPLMLSGTLM LLTALLVTAK VKMKKS


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