Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Putative outer membrane protein assembly factor TP_0326 (TP_92)

 TP326_TREPA             Reviewed;         837 AA.
O83346;
09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
09-DEC-2015, sequence version 2.
22-NOV-2017, entry version 98.
RecName: Full=Putative outer membrane protein assembly factor TP_0326 {ECO:0000305|PubMed:21488980};
AltName: Full=TP_92 {ECO:0000303|PubMed:12904373};
Flags: Precursor;
Name=tp92; OrderedLocusNames=TP_0326; ORFNames=TPANIC_0326;
Treponema pallidum (strain Nichols).
Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Treponema.
NCBI_TaxID=243276;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Nichols;
PubMed=9665876; DOI=10.1126/science.281.5375.375;
Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M.,
Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D.,
Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M.,
Weidman J.F., Smith H.O., Venter J.C.;
"Complete genome sequence of Treponema pallidum, the syphilis
spirochete.";
Science 281:375-388(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Nichols;
PubMed=24058545; DOI=10.1371/journal.pone.0074319;
Petrosova H., Pospisilova P., Strouhal M., Cejkova D., Zobanikova M.,
Mikalova L., Sodergren E., Weinstock G.M., Smajs D.;
"Resequencing of Treponema pallidum ssp. pallidum strains Nichols and
SS14: correction of sequencing errors resulted in increased separation
of syphilis treponeme subclusters.";
PLoS ONE 8:E74319-E74319(2013).
[3]
BIOTECHNOLOGY.
PubMed=12904373; DOI=10.1128/JCM.41.8.3668-3674.2003;
Van Voorhis W.C., Barrett L.K., Lukehart S.A., Schmidt B.,
Schriefer M., Cameron C.E.;
"Serodiagnosis of syphilis: antibodies to recombinant Tp0453, Tp92,
and Gpd proteins are sensitive and specific indicators of infection by
Treponema pallidum.";
J. Clin. Microbiol. 41:3668-3674(2003).
[4]
FUNCTION IN HOST IMMUNE SYSTEM RESPONSE, SUBUNIT, SUBCELLULAR
LOCATION, INDUCTION, DOMAIN, AND TOPOLOGY.
STRAIN=Nichols-Farmington;
PubMed=21488980; DOI=10.1111/j.1365-2958.2011.07662.x;
Desrosiers D.C., Anand A., Luthra A., Dunham-Ems S.M., LeDoyt M.,
Cummings M.A., Eshghi A., Cameron C.E., Cruz A.R., Salazar J.C.,
Caimano M.J., Radolf J.D.;
"TP0326, a Treponema pallidum beta-barrel assembly machinery A (BamA)
orthologue and rare outer membrane protein.";
Mol. Microbiol. 80:1496-1515(2011).
[5]
BIOTECHNOLOGY.
STRAIN=Nichols;
PubMed=23100335; DOI=10.1128/JCM.01390-12;
Smith B.C., Simpson Y., Morshed M.G., Cowen L.L., Hof R.,
Wetherell C., Cameron C.E.;
"New proteins for a new perspective on syphilis diagnosis.";
J. Clin. Microbiol. 51:105-111(2013).
[6]
FUNCTION IN HOST IMMUNE SYSTEM RESPONSE, SUBCELLULAR LOCATION,
STRUCTURE MODEL, TOPOLOGY, AND EXPRESSION IN E.COLI.
STRAIN=Nichols-Farmington;
PubMed=25825429; DOI=10.1128/JB.00086-15;
Luthra A., Anand A., Hawley K.L., LeDoyt M., La Vake C.J.,
Caimano M.J., Cruz A.R., Salazar J.C., Radolf J.D.;
"A homology model reveals novel structural features and an
immunodominant surface loop/opsonic target in the Treponema pallidum
BamA ortholog TP_0326.";
J. Bacteriol. 197:1906-1920(2015).
-!- FUNCTION: Might be part of the outer membrane protein assembly
complex, which is involved in assembly and insertion of beta-
barrel proteins into the outer membrane.
{ECO:0000250|UniProtKB:P0A940}.
-!- FUNCTION: Both rabbit immune serum and rabbit antiserum specific
for extracytoplasmic loop L4 promote bacteria internalization by
rabbit peritoneal macrophages. Pools of human syphilitic sera from
the USA and Columbia recognize both the N-terminal POTRA-
containing and C-terminal beta-barrel domains as well as loop L4,
showing this protein stimulates the immune system in both humans
and rabbits (PubMed:25825429). {ECO:0000269|PubMed:21488980,
ECO:0000269|PubMed:25825429}.
-!- SUBUNIT: Part of 2 complexes of about 239 and 164 kDa.
{ECO:0000269|PubMed:21488980}.
-!- SUBCELLULAR LOCATION: Cell outer membrane
{ECO:0000269|PubMed:21488980, ECO:0000269|PubMed:25825429}.
Note=The C-terminal beta-barrel domain is exposed on the outer
surface of the outer membrane (PubMed:21488980). Antibody labeling
by antisera to either the periplasmic or membrane domain occurs in
a punctate fashion over the length of the spirochete; detection by
periplasmic POTRA-domain-specific antibodies only occurs after
mild detergent solubilization (PubMed:25825429).
{ECO:0000269|PubMed:21488980, ECO:0000269|PubMed:25825429}.
-!- INDUCTION: Transcribed at low levels, estimated to be present at
about 100 molecules per cell (at protein level).
{ECO:0000269|PubMed:21488980}.
-!- DOMAIN: The N-terminus (residues 22-369) partitions into an
aqueous phase and is predicted to be periplasmic, while the C-
terminus (residues 426-837) partitions into Triton X-114 and has
the hallmarks of a beta-stranded protein, suggesting it is
membraneous. {ECO:0000269|PubMed:21488980}.
-!- BIOTECHNOLOGY: Recognized by sera from 42/43 syphilis patients, it
shows promise as a diagnostic antigen (PubMed:12904373). In
further diagnostic tests for syphilis this protein exhibits a
sensitivity of 86% and a specificity of 99%. A fusion with TP0453
(residues 32-287 of TP0453 fused N-terminally to residues 38-450
of TP0326) exhibits a sensitivity of 98% and a specificity of 99%,
and exhibits superior accuracy in classifying analytical false-
positive samples than a commericially available test, making it a
better candiate for diagnosis (PubMed:23100335).
{ECO:0000269|PubMed:12904373, ECO:0000269|PubMed:23100335}.
-!- MISCELLANEOUS: The outer membrane of T.pallidum has no
lipopolysaccharides, few proteins and is a fluid and very fragile
bilayer. Its lack of surface antigenicity is thought to contribute
to the ability of the pathogen to evade the human immune system.
{ECO:0000305}.
-!- SIMILARITY: Belongs to the BamA family. {ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAC65313.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Sequence=AGN75525.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; AE000520; AAC65313.1; ALT_INIT; Genomic_DNA.
EMBL; CP004010; AGN75525.1; ALT_INIT; Genomic_DNA.
PIR; A71339; A71339.
RefSeq; WP_014342788.1; NC_021490.2.
ProteinModelPortal; O83346; -.
SMR; O83346; -.
STRING; 243276.TP0326; -.
EnsemblBacteria; AAC65313; AAC65313; TP_0326.
EnsemblBacteria; AGN75525; AGN75525; TPANIC_0326.
KEGG; tpa:TP_0326; -.
KEGG; tpw:TPANIC_0326; -.
PATRIC; fig|243276.9.peg.324; -.
eggNOG; ENOG4105E1Z; Bacteria.
eggNOG; COG4775; LUCA.
KO; K07277; -.
Proteomes; UP000000811; Chromosome.
Proteomes; UP000014259; Chromosome.
GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0071709; P:membrane assembly; IEA:InterPro.
InterPro; IPR000184; Bac_surfAg_D15.
InterPro; IPR010827; BamA/TamA_POTRA.
InterPro; IPR023707; OM_assembly_BamA.
InterPro; IPR034746; POTRA.
PANTHER; PTHR12815:SF23; PTHR12815:SF23; 2.
Pfam; PF01103; Bac_surface_Ag; 1.
Pfam; PF07244; POTRA; 4.
PIRSF; PIRSF006076; OM_assembly_OMP85; 1.
TIGRFAMs; TIGR03303; OM_YaeT; 1.
PROSITE; PS51779; POTRA; 5.
1: Evidence at protein level;
Cell outer membrane; Complete proteome; Membrane; Reference proteome;
Repeat; Signal; Transmembrane; Transmembrane beta strand.
SIGNAL 1 21 {ECO:0000255}.
CHAIN 22 837 Putative outer membrane protein assembly
factor TP_0326.
/FTId=PRO_5004161024.
TOPO_DOM 22 433 Periplasmic. {ECO:0000305}.
TRANSMEM 434 442 Beta stranded; Name=1.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 443 450 Extracellular; loop L1. {ECO:0000305}.
TRANSMEM 451 461 Beta stranded; Name=2.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 462 468 Periplasmic. {ECO:0000305}.
TRANSMEM 469 476 Beta stranded; Name=3.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 477 478 Extracellular; loop L2. {ECO:0000305}.
TRANSMEM 479 489 Beta stranded; Name=4.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 490 499 Periplasmic. {ECO:0000305}.
TRANSMEM 500 520 Beta stranded; Name=5.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 521 530 Extracellular; loop L3. {ECO:0000305}.
TRANSMEM 531 543 Beta stranded; Name=6.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 544 554 Periplasmic. {ECO:0000305}.
TRANSMEM 555 568 Beta stranded; Name=7.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 569 601 Extracellular; loop L4.
{ECO:0000305|PubMed:25825429}.
TRANSMEM 602 615 Beta stranded; Name=8.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 616 623 Periplasmic. {ECO:0000305}.
TRANSMEM 624 636 Beta stranded; Name=9.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 637 644 Extracellular; loop L5. {ECO:0000305}.
TRANSMEM 645 658 Beta stranded; Name=10.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 659 667 Periplasmic. {ECO:0000305}.
TRANSMEM 668 682 Beta stranded; Name=11.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 683 724 Extracellular; loop L6. {ECO:0000305}.
TRANSMEM 725 736 Beta stranded; Name=12.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 737 741 Periplasmic. {ECO:0000305}.
TRANSMEM 742 756 Beta stranded; Name=13.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 757 786 Extracellular; loop L7. {ECO:0000305}.
TRANSMEM 787 799 Beta stranded; Name=14.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 800 802 Periplasmic. {ECO:0000305}.
TRANSMEM 803 814 Beta stranded; Name=15.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 815 824 Extracellular; loop L8. {ECO:0000305}.
TRANSMEM 825 829 Beta stranded; Name=16.
{ECO:0000303|PubMed:25825429}.
TOPO_DOM 830 837 Periplasmic. {ECO:0000305}.
DOMAIN 31 105 POTRA 1. {ECO:0000255|PROSITE-
ProRule:PRU01115,
ECO:0000305|PubMed:21488980}.
DOMAIN 106 182 POTRA 2. {ECO:0000255|PROSITE-
ProRule:PRU01115}.
DOMAIN 185 273 POTRA 3. {ECO:0000255|PROSITE-
ProRule:PRU01115}.
DOMAIN 276 354 POTRA 4. {ECO:0000255|PROSITE-
ProRule:PRU01115}.
DOMAIN 357 430 POTRA 5. {ECO:0000255|PROSITE-
ProRule:PRU01115}.
COMPBIAS 759 784 Ser-rich. {ECO:0000255|PROSITE-
ProRule:PRU00016}.
SEQUENCE 837 AA; 94267 MW; 10DA39487F828FBC CRC64;
MLKKASAFLI ASCCVMSLAW AQANDNWYEG KPISAISFEG LEYIARGQLD TIFSQYKGQK
WTYELYLEIL QKVYDLEYFS EVSPKAVPTD PEYQYVMLQF TVKERPSVKG IKMVGNSQIR
SGDLLSKILL KKGDIYNEVK MKVDQESLRR HYLDQGYAAV KISCEAKTEA GGVVVQFTIQ
EGKQTVVSRI QFKGNKAFTE SVLKKVLSTQ EARFLTSGVF KENALEADKA AVHSYYAERG
YIDARVEGVA KTVDKKTDAS RNLVTLTYTV VEGEQYRYGG VTIVGNQIFS TEELQAKIRL
KRGAIMNMVA FEQGFQALAD AYFENGYTSN YLNKEEHRDT AEKTLSFKIT VVERERSHVE
HIIIKGTKNT KDEVILREML LKPGDVFSKS KFTDSLRNLF NLRYFSSLVP DVRPGSEQDL
VDIILNVEEQ STANVQFGVT FSGVGEAGTF PLSLFCQWEE KNFLGKGNEI SVNATLGSEA
QSLKLGYVER WFLGSPLTVG FDFELTHKNL FVYRAGSYGN GLPHPYTSRE QWASSPGLAE
SFRLKYSRFE SAIGAHTGYQ WYPRYAVIRV NGGVDFRVVK NFYDKDNNQP FDLTVKEQLN
WTSINSFWTS VSFDGRDFAY DPSSGWFLGQ RCTFNGLVPF LEKEHSFRSD TKAEFYVTLL
NYPVSAVWNL KFVLAFYTGV SVQTYYGRRK SENGKGNGVR SGALVIDGVL VGRGWSEDAK
KNTGDLLLHH WIEFRWPLAH GIVSFDFFFD AAMVYNIESQ SPNGSSSASS SSSSSSSSSR
TTSSEGLYKM SYGPGLRFTL PQFPLKLAFA NTFTSPGGIP KTKKNWNFVL SFTVNNL


Related products :

Catalog number Product name Quantity
bs-2086P Peptides: ompF(putative outer membrane porin F protein) Protein Length:12-25 amino acids. 200ug lyophilized
bs-2086R Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody 100ug Lyophilized
bs-2086R-Cy5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy5 Conjugated 100ug
bs-2086R-PE-Cy5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy5 Conjugated 100ug
bs-2086R Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Unconjugated 100ug
bs-2086R-HRP Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, HRP Conjugated 100ug
bs-2086R-PE-Cy3 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy3 Conjugated 100ug
bs-2086R-Cy7 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy7 Conjugated 100ug
bs-2086R-Cy5.5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy5.5 Conjugated 100ug
bs-2086R-Cy3 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy3 Conjugated 100ug
bs-2086R-PE Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE Conjugated 100ug
bs-2086R-PE-Cy7 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy7 Conjugated 100ug
bs-2086R-PE-Cy5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy5 conjugated Isotype: IgG 100ug Lyophilized
bs-2086R-HRP Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, HRP conjugated Isotype: IgG 100ug Lyophilized
bs-2086R-Cy7 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy7 conjugated Isotype: IgG 100ug Lyophilized
bs-2086R-Cy5.5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy5.5 conjugated, Isotype: IgG 100ug Lyophilized
bs-2086R-Cy5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy5 conjugated, Isotype: IgG 100ug Lyophilized
bs-2086R-Cy3 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy3 conjugated, Isotype: IgG 100ug Lyophilized
bs-2086R-PE-Cy5.5 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy5.5 conjugated Isotype: IgG 100ug Lyophilized
bs-2086R-PE-Cy7 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, PE-Cy7 conjugated Isotype: IgG 100ug Lyophilized
bs-2086R-Alexa488 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Alexa 488 Conjugated 100ug
bs-2086R-Cy3 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy3 Conjugated, Isotype IgG, Application Flow-Cyt(1 20-100), IF(1 50-200) 100ug Lyophilized
bs-2086R-Cy7 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Cy7 Conjugated, Isotype IgG, Application Flow-Cyt(1 20-100), IF(1 50-200) 100ug Lyophilized
bs-2086R-A647 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Alexa Fluor 647 conjugated,Isotype: IgG 100ug Lyophilized
bs-2086R-A350 Rabbit Anti-ompF(putative outer membrane porin F protein) Polyclonal Antibody, Alexa Fluor 350 conjugated,Isotype: IgG 100ug Lyophilized


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur