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Questin oxidase (EC 1.-.-.-) (Trypacidin synthesis protein K)

 TPCI_ASPFU              Reviewed;         445 AA.
Q4WQY9;
07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
05-JUL-2005, sequence version 1.
05-DEC-2018, entry version 52.
RecName: Full=Questin oxidase {ECO:0000250|UniProtKB:Q0CCX5};
EC=1.-.-.- {ECO:0000250|UniProtKB:Q0CCX5};
AltName: Full=Trypacidin synthesis protein K {ECO:0000303|PubMed:26278536};
Name=tpcI {ECO:0000303|PubMed:26242966};
Synonyms=tynI {ECO:0000303|PubMed:26278536}; ORFNames=AFUA_4G14500;
Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
A1100) (Aspergillus fumigatus).
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus.
NCBI_TaxID=330879;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
PubMed=16372009; DOI=10.1038/nature04332;
Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S.,
Arroyo J., Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W.,
Bowyer P., Chen D., Collins M., Coulsen R., Davies R., Dyer P.S.,
Farman M.L., Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R.,
Fosker N., Fraser A., Garcia J.L., Garcia M.J., Goble A.,
Goldman G.H., Gomi K., Griffith-Jones S., Gwilliam R., Haas B.J.,
Haas H., Harris D.E., Horiuchi H., Huang J., Humphray S., Jimenez J.,
Keller N., Khouri H., Kitamoto K., Kobayashi T., Konzack S.,
Kulkarni R., Kumagai T., Lafton A., Latge J.-P., Li W., Lord A.,
Lu C., Majoros W.H., May G.S., Miller B.L., Mohamoud Y., Molina M.,
Monod M., Mouyna I., Mulligan S., Murphy L.D., O'Neil S., Paulsen I.,
Penalva M.A., Pertea M., Price C., Pritchard B.L., Quail M.A.,
Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
Ronning C.M., Rutter S., Salzberg S.L., Sanchez M.,
Sanchez-Ferrero J.C., Saunders D., Seeger K., Squares R., Squares S.,
Takeuchi M., Tekaia F., Turner G., Vazquez de Aldana C.R., Weidman J.,
White O., Woodward J.R., Yu J.-H., Fraser C.M., Galagan J.E., Asai K.,
Machida M., Hall N., Barrell B.G., Denning D.W.;
"Genomic sequence of the pathogenic and allergenic filamentous fungus
Aspergillus fumigatus.";
Nature 438:1151-1156(2005).
[2]
FUNCTION, AND TISSUE SPECIFICITY.
PubMed=22319557; DOI=10.1371/journal.pone.0029906;
Gauthier T., Wang X., Sifuentes Dos Santos J., Fysikopoulos A.,
Tadrist S., Canlet C., Artigot M.P., Loiseau N., Oswald I.P., Puel O.;
"Trypacidin, a spore-borne toxin from Aspergillus fumigatus, is
cytotoxic to lung cells.";
PLoS ONE 7:E29906-E29906(2012).
[3]
FUNCTION.
PubMed=26278536; DOI=10.1007/s00253-015-6898-1;
Mattern D.J., Schoeler H., Weber J., Novohradska S., Kraibooj K.,
Dahse H.M., Hillmann F., Valiante V., Figge M.T., Brakhage A.A.;
"Identification of the antiphagocytic trypacidin gene cluster in the
human-pathogenic fungus Aspergillus fumigatus.";
Appl. Microbiol. Biotechnol. 99:10151-10161(2015).
[4]
FUNCTION.
PubMed=26242966; DOI=10.1111/1462-2920.13007;
Throckmorton K., Lim F.Y., Kontoyiannis D.P., Zheng W., Keller N.P.;
"Redundant synthesis of a conidial polyketide by two distinct
secondary metabolite clusters in Aspergillus fumigatus.";
Environ. Microbiol. 18:246-259(2016).
-!- FUNCTION: Questin oxidase; part of the gene cluster that mediates
the biosynthesis of trypacidin, a mycotoxin with antiprotozoal
activity and that plays a role in the infection process
(PubMed:26278536, PubMed:26242966). The pathway begins with the
synthesis of atrochrysone thioester by the polyketide synthase
(PKS) tpcC (PubMed:26242966). The atrochrysone carboxyl ACP
thioesterase tpcB then breaks the thioester bond and releases the
atrochrysone carboxylic acid from tpcC (PubMed:26242966). The
decarboxylase tpcK converts atrochrysone carboxylic acid to
atrochrysone which is further reduced into emodin anthrone
(PubMed:26242966). The next step is performed by the emodin
anthrone oxygenase tpcL that catalyzes the oxidation of
emodinanthrone to emodin (PubMed:26242966). Emodin O-
methyltransferase encoded by tpcA catalyzes methylation of the 8-
hydroxy group of emodin to form questin (PubMed:26242966). Ring
cleavage of questin by questin oxidase tpcI leads to
desmethylsulochrin via several intermediates including questin
epoxide (By similarity). Another methylation step catalyzed by
tpcM leads to the formation of sulochrin which is further
converted to monomethylsulfochrin by tpcH. Finally, the tpcJ
catalyzes the conversion of monomethylsulfochrin to trypacidin
(PubMed:26242966). Trypacidin is toxic for human pulmonary and
bronchial epithelial cells by initiating the intracellular
formation of nitric oxide (NO) and hydrogen peroxide (H(2)O(2)),
thus triggering host necrotic cell death (PubMed:22319557). The
trypacidin pathway is also able to produce endocrocin via a
distinct route from the endocrocin Enc pathway (PubMed:26242966).
{ECO:0000250|UniProtKB:Q0CCX8, ECO:0000269|PubMed:22319557,
ECO:0000269|PubMed:26242966, ECO:0000269|PubMed:26278536}.
-!- COFACTOR:
Name=NADPH; Xref=ChEBI:CHEBI:57783;
Evidence={ECO:0000250|UniProtKB:Q0CCX5};
-!- PATHWAY: Secondary metabolite biosynthesis.
{ECO:0000305|PubMed:26242966}.
-!- TISSUE SPECIFICITY: Specifically expressed in conidia
(PubMed:22319557). {ECO:0000305|PubMed:22319557}.
-!- SIMILARITY: Belongs to the questin oxidase family. {ECO:0000305}.
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EMBL; AAHF01000005; EAL89345.1; -; Genomic_DNA.
RefSeq; XP_751383.1; XM_746290.1.
EnsemblFungi; EAL89345; EAL89345; AFUA_4G14500.
GeneID; 3509607; -.
KEGG; afm:AFUA_4G14500; -.
EuPathDB; FungiDB:Afu4g14500; -.
HOGENOM; HOG000160151; -.
InParanoid; Q4WQY9; -.
OMA; MGWKELY; -.
OrthoDB; EOG092C1PZR; -.
Proteomes; UP000002530; Chromosome 4.
Proteomes; UP000002530; Unassembled WGS sequence.
GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
GO; GO:0044550; P:secondary metabolite biosynthetic process; IGC:AspGD.
InterPro; IPR025337; Questin_oxidase-like.
PANTHER; PTHR35870; PTHR35870; 1.
Pfam; PF14027; Questin_oxidase; 1.
2: Evidence at transcript level;
Complete proteome; Oxidoreductase; Reference proteome.
CHAIN 1 445 Questin oxidase.
/FTId=PRO_0000437101.
SEQUENCE 445 AA; 49467 MW; 8B8D08C41DF03C78 CRC64;
MATASQIRLR PRLGVARATD TNPDGALEAA NRLLQKNHNE HHMFWRSVAG HNHVAHSVLN
TLALGGGPGD LQRAFDDGAE IQVPIPPMDR DAAASLSTPD QFRARMGSLE QYPNFLVFFT
KEIETRGYPA VVIEYCFSGT SIAESMFANL FEGLYHPLIH LALGIEFDQP SIVAEGLAQA
ACHDSMNIEP FLFGTDKLAK TQDAPIDLNT PLLSLFHAVR DNEVLRAAAH RDHGDGVARV
RDGILGRARE EISLIAARFR VDVDELDHRA AEMISCAAYL AGSAQRPGKA RKIDFFHLHA
VTASLGLIVL LQQPWVTPEQ KARLIEWKAR VDLVWYAASG AVELRRKDIV DYRPRHGLTW
DSLYQAVRRV HDDGHLAKFI RALKAGEQIS RPFEQGKRAE AFPIKGSMWL RIAQMAYDSS
AGRPIEEKWI WGAGFEPNWA TVPAV


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