Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

RAF proto-oncogene serine/threonine-protein kinase (EC 2.7.11.1) (C-MIL) (C-RAF) (MIL proto-oncogene serine/threonine-protein kinase) (RAF-1)

 RAF1_CHICK              Reviewed;         647 AA.
P05625; Q90893; Q90894;
01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
01-NOV-1988, sequence version 1.
12-SEP-2018, entry version 164.
RecName: Full=RAF proto-oncogene serine/threonine-protein kinase;
EC=2.7.11.1;
AltName: Full=C-MIL;
AltName: Full=C-RAF;
AltName: Full=MIL proto-oncogene serine/threonine-protein kinase;
AltName: Full=RAF-1;
Name=RAF1; Synonyms=MIL;
Gallus gallus (Chicken).
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes;
Phasianidae; Phasianinae; Gallus.
NCBI_TaxID=9031;
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
TISSUE=Erythroblast;
PubMed=3285296;
Koenen M., Sippel A.E., Trachmann C., Bister K.;
"Primary structure of the chicken c-mil protein: identification of
domains shared with or absent from the retroviral v-mil protein.";
Oncogene 2:179-185(1988).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-647.
PubMed=2998016; DOI=10.1016/0042-6822(85)90376-9;
Jansen H.W., Bister K.;
"Nucleotide sequence analysis of the chicken gene c-mil, the
progenitor of the retroviral oncogene v-mil.";
Virology 143:359-367(1985).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 230-330.
PubMed=3002017; DOI=10.1016/0042-6822(85)90257-0;
Flordellis C.S., Kan N.C., Lautenberger J.A., Samuel K.P., Garon C.F.,
Papas T.S.;
"Analysis of the cellular proto-oncogene mht/raf: relationship to the
5' sequences of v-mht in avian carcinoma virus MH2 and v-raf in murine
sarcoma virus 3611.";
Virology 141:267-274(1985).
[4]
NUCLEOTIDE SEQUENCE [MRNA] OF 275-283 (ISOFORM 2), AND ALTERNATIVE
SPLICING.
TISSUE=Embryo;
PubMed=2837658; DOI=10.1128/MCB.8.4.1835;
Dozier C., Denhez F., Henry C., Coll J., Begue A., Quatannens B.,
Saule S., Stehelin D.;
"Alternative splicing of RNAs transcribed from the chicken c-mil
gene.";
Mol. Cell. Biol. 8:1835-1838(1988).
[5]
PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, AND
TISSUE SPECIFICITY.
PubMed=1886707;
Dozier C., Ansieau S., Ferreira E., Coll J., Stehelin D.;
"An alternatively spliced c-mil/raf mRNA is predominantly expressed in
chicken muscular tissues and conserved among vertebrate species.";
Oncogene 6:1307-1311(1991).
-!- FUNCTION: Serine/threonine-protein kinase that acts as a
regulatory link between the membrane-associated Ras GTPases and
the MAPK/ERK cascade, and this critical regulatory link functions
as a switch determining cell fate decisions. RAF1 activation
initiates a mitogen-activated protein kinase (MAPK) cascade that
comprises a sequential phosphorylation of the dual-specific MAPK
kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular
signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) (By
similarity). {ECO:0000250}.
-!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell membrane
{ECO:0000250}.
-!- ALTERNATIVE PRODUCTS:
Event=Alternative splicing; Named isoforms=2;
Name=1; Synonyms=6C;
IsoId=P05625-1; Sequence=Displayed;
Name=2; Synonyms=1A;
IsoId=P05625-2; Sequence=VSP_034628;
-!- TISSUE SPECIFICITY: Isoform 1 was present in all tissues tested:
skeletal muscle, intestine, brain, gizzard, heart, lung, kidney,
bone marrow, spleen and bursa of Fabricius. Isoform 2 was only
detected in brain, heart and skeletal muscle. In brain and heart
isoform 1 is more abundant than isoform 2. In skeletal muscle
isoform 2 is more abundant than isoform 1.
{ECO:0000269|PubMed:1886707}.
-!- PTM: Phosphorylation at Ser-259 inactivates kinase activity.
Dephosphorylation of Ser-259 by a complex containing protein
phosphatase 1 relieves inactivation, leading to stimulate RAF1
activity (By similarity). {ECO:0000250}.
-!- SIMILARITY: Belongs to the protein kinase superfamily. TKL Ser/Thr
protein kinase family. RAF subfamily. {ECO:0000305}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution (CC BY 4.0) License
-----------------------------------------------------------------------
EMBL; X07017; CAA30069.1; -; mRNA.
EMBL; K03269; AAA48952.1; -; Genomic_DNA.
EMBL; K03259; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03260; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03261; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03262; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03263; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03264; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03265; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03266; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03267; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03268; AAA48952.1; JOINED; Genomic_DNA.
EMBL; K03048; AAA48951.1; -; Genomic_DNA.
EMBL; K03047; AAA48951.1; JOINED; Genomic_DNA.
EMBL; M19461; AAA48953.1; -; mRNA.
EMBL; X55430; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PIR; I50382; I50382.
PIR; S00644; S00644.
RefSeq; NP_990638.1; NM_205307.2. [P05625-1]
RefSeq; XP_015148495.1; XM_015293009.1. [P05625-2]
RefSeq; XP_015148496.1; XM_015293010.1. [P05625-2]
RefSeq; XP_015148497.1; XM_015293011.1. [P05625-2]
RefSeq; XP_015148498.1; XM_015293012.1. [P05625-2]
RefSeq; XP_015148499.1; XM_015293013.1. [P05625-1]
UniGene; Gga.42842; -.
ProteinModelPortal; P05625; -.
SMR; P05625; -.
BioGrid; 676505; 1.
STRING; 9031.ENSGALP00000007991; -.
iPTMnet; P05625; -.
PaxDb; P05625; -.
PRIDE; P05625; -.
Ensembl; ENSGALT00000033956; ENSGALP00000033314; ENSGALG00000004998. [P05625-1]
Ensembl; ENSGALT00000049372; ENSGALP00000046736; ENSGALG00000004998. [P05625-2]
GeneID; 396245; -.
KEGG; gga:396245; -.
CTD; 5894; -.
eggNOG; KOG0193; Eukaryota.
eggNOG; ENOG410Y4UP; LUCA.
GeneTree; ENSGT00900000140880; -.
HOGENOM; HOG000252972; -.
HOVERGEN; HBG001886; -.
InParanoid; P05625; -.
KO; K04366; -.
OMA; HQFIRKT; -.
OrthoDB; EOG091G09SB; -.
PhylomeDB; P05625; -.
TreeFam; TF317006; -.
BRENDA; 2.7.11.1; 1306.
Reactome; R-GGA-2672351; Stimuli-sensing channels.
Reactome; R-GGA-392517; Rap1 signalling.
Reactome; R-GGA-430116; GP1b-IX-V activation signalling.
Reactome; R-GGA-442742; CREB phosphorylation through the activation of Ras.
Reactome; R-GGA-5621575; CD209 (DC-SIGN) signaling.
Reactome; R-GGA-5673000; RAF activation.
Reactome; R-GGA-5674135; MAP2K and MAPK activation.
Reactome; R-GGA-5674499; Negative feedback regulation of MAPK pathway.
Reactome; R-GGA-5675221; Negative regulation of MAPK pathway.
PRO; PR:P05625; -.
Proteomes; UP000000539; Chromosome 12.
Bgee; ENSGALG00000004998; Expressed in 10 organ(s), highest expression level in skeletal muscle tissue.
GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
GO; GO:0005829; C:cytosol; IBA:GO_Central.
GO; GO:0005794; C:Golgi apparatus; IEA:Ensembl.
GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
GO; GO:0031143; C:pseudopodium; IEA:Ensembl.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
GO; GO:0004709; F:MAP kinase kinase kinase activity; IBA:GO_Central.
GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO; GO:0031434; F:mitogen-activated protein kinase kinase binding; IBA:GO_Central.
GO; GO:0017016; F:Ras GTPase binding; IEA:Ensembl.
GO; GO:0007190; P:activation of adenylate cyclase activity; IEA:Ensembl.
GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
GO; GO:0071550; P:death-inducing signaling complex assembly; IEA:Ensembl.
GO; GO:0060324; P:face development; IEA:Ensembl.
GO; GO:0035773; P:insulin secretion involved in cellular response to glucose stimulus; IEA:Ensembl.
GO; GO:0045104; P:intermediate filament cytoskeleton organization; IEA:Ensembl.
GO; GO:0007275; P:multicellular organism development; IBA:GO_Central.
GO; GO:0043066; P:negative regulation of apoptotic process; IBA:GO_Central.
GO; GO:0008285; P:negative regulation of cell proliferation; IEA:Ensembl.
GO; GO:1902042; P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; IEA:Ensembl.
GO; GO:0031333; P:negative regulation of protein complex assembly; IEA:Ensembl.
GO; GO:0009968; P:negative regulation of signal transduction; IBA:GO_Central.
GO; GO:0048011; P:neurotrophin TRK receptor signaling pathway; IEA:Ensembl.
GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IBA:GO_Central.
GO; GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IEA:Ensembl.
GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
GO; GO:0035994; P:response to muscle stretch; IEA:Ensembl.
GO; GO:0035019; P:somatic stem cell population maintenance; IEA:Ensembl.
GO; GO:0048538; P:thymus development; IEA:Ensembl.
GO; GO:0030878; P:thyroid gland development; IEA:Ensembl.
CDD; cd00029; C1; 1.
InterPro; IPR020454; DAG/PE-bd.
InterPro; IPR011009; Kinase-like_dom_sf.
InterPro; IPR002219; PE/DAG-bd.
InterPro; IPR000719; Prot_kinase_dom.
InterPro; IPR017441; Protein_kinase_ATP_BS.
InterPro; IPR003116; RBD_dom.
InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
InterPro; IPR008271; Ser/Thr_kinase_AS.
InterPro; IPR029071; Ubiquitin-like_domsf.
Pfam; PF00130; C1_1; 1.
Pfam; PF07714; Pkinase_Tyr; 1.
Pfam; PF02196; RBD; 1.
PRINTS; PR00008; DAGPEDOMAIN.
SMART; SM00109; C1; 1.
SMART; SM00455; RBD; 1.
SMART; SM00220; S_TKc; 1.
SUPFAM; SSF54236; SSF54236; 1.
SUPFAM; SSF56112; SSF56112; 1.
PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PROSITE; PS50898; RBD; 1.
PROSITE; PS00479; ZF_DAG_PE_1; 1.
PROSITE; PS50081; ZF_DAG_PE_2; 1.
2: Evidence at transcript level;
Alternative splicing; ATP-binding; Cell membrane; Complete proteome;
Cytoplasm; Kinase; Membrane; Metal-binding; Nucleotide-binding;
Phosphoprotein; Proto-oncogene; Reference proteome;
Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.
CHAIN 1 647 RAF proto-oncogene serine/threonine-
protein kinase.
/FTId=PRO_0000086599.
DOMAIN 56 131 RBD. {ECO:0000255|PROSITE-
ProRule:PRU00262}.
DOMAIN 349 609 Protein kinase. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
ZN_FING 138 184 Phorbol-ester/DAG-type.
{ECO:0000255|PROSITE-ProRule:PRU00226}.
NP_BIND 355 363 ATP. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
ACT_SITE 468 468 Proton acceptor. {ECO:0000255|PROSITE-
ProRule:PRU00159, ECO:0000255|PROSITE-
ProRule:PRU10027}.
BINDING 375 375 ATP. {ECO:0000255|PROSITE-
ProRule:PRU00159}.
MOD_RES 43 43 Phosphoserine. {ECO:0000250}.
MOD_RES 259 259 Phosphoserine. {ECO:0000250}.
MOD_RES 268 268 Phosphothreonine; by autocatalysis.
{ECO:0000250}.
MOD_RES 338 338 Phosphoserine. {ECO:0000250}.
MOD_RES 499 499 Phosphoserine. {ECO:0000250}.
MOD_RES 621 621 Phosphoserine. {ECO:0000250}.
VAR_SEQ 278 278 E -> ENNSLNASPSSWCRRFCLRGR (in isoform
2). {ECO:0000303|PubMed:2837658}.
/FTId=VSP_034628.
CONFLICT 549 549 S -> F (in Ref. 2; AAA48952).
{ECO:0000305}.
SEQUENCE 647 AA; 73125 MW; 8E1443667312DFC0 CRC64;
MEHIQGAWKT ISNGFGLKDS VFDGPNCISP TIVQQFGYQR RASDDGKISD TSKTSNTIRV
FLPNKQRTVV NVRNGMTLHD CLMKALKVRG LQPECCAVFR LVTEPKGKKV RLDWNTDAAS
LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV
PTMCVDWSNI RQLLLFPNSN ISDSGVPALP PLTMRRMRES VSRIPVSSQH RYSTPHVFTF
NTSNPSSEGT LSQRQRSTST PNVHMVSTTM PVDSRIIEDA IRNHSESASP SALSGSPNNM
SPTGWSQPKT PVPAQRERAP GTNTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF
GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV
TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL
TVKIGDFGLA TVKSRWSGSQ QVEQPTGSIL WMAPEVIRMQ DSNPFSFQSD VYSYGIVLYE
LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCLK KVREERPLFP
QILSSIELLQ HSLPKINRSA SEPSLHRASH TEDINSCTLT STRLPVF


Related products :

Catalog number Product name Quantity
G4694 Proto-oncogene serine threonine-protein kinase mos (MOS), Rat, ELISA Kit 96T
G4693 Proto-oncogene serine threonine-protein kinase mos (MOS), Pig, ELISA Kit 96T
G6186 RAF proto-oncogene serine threonine-protein kinase (RAF1), Rat, ELISA Kit 96T
G4696 Proto-oncogene serine threonine-protein kinase pim-1 (PIM1), Cat, ELISA Kit 96T
G4692 Proto-oncogene serine threonine-protein kinase mos (MOS), Mouse, ELISA Kit 96T
G4691 Proto-oncogene serine threonine-protein kinase mos (MOS), Human, ELISA Kit 96T
G4690 Proto-oncogene serine threonine-protein kinase mos (MOS), Chicken, ELISA Kit 96T
RAF1_HUMAN Human ELISA Kit FOR RAF proto-oncogene serine per threonine-protein kinase 96T
E0726r Bovine ELISA Kit FOR RAF proto-oncogene serine per threonine-protein kinase 96T
E0812r Mouse ELISA Kit FOR Proto-oncogene serine per threonine-protein kinase mos 96T
SVOP_BOVIN Bovine ELISA Kit FOR RAF proto-oncogene serine per threonine-protein kinase 96T
G4699 Proto-oncogene serine threonine-protein kinase pim-1 (PIM1), Rat, ELISA Kit 96T
E2796Hu Human C-Raf Proto Oncogene Serine per Threonine Protein Kinase (CRAF)ELISA Kit 96T
E2796Hu Human C-Raf Proto Oncogene Serine-Threonine Protein Kinase (CRAF)ELISA Kit 48T
UT-E05024 Human C-Raf Proto Oncogene Serine Threonine Protein Kinase (CRAF) ELISA Kit 96T
G4698 Proto-oncogene serine threonine-protein kinase pim-1 (PIM1), Mouse, ELISA Kit 96T
G4697 Proto-oncogene serine threonine-protein kinase pim-1 (PIM1), Human, ELISA Kit 96T
G4695 Proto-oncogene serine threonine-protein kinase pim-1 (PIM1), Bovine, ELISA Kit 96T
YHB0856Hu Human C-Raf Proto Oncogene Serine-Threonine Protein Kinase (CRAF)ELISA Kit 96T
E2796Hu Human C-Raf Proto Oncogene Serine-Threonine Protein Kinase (CRAF)ELISA Kit 96T
CSB-E11825h Human Proto-oncogene serine per threonine-protein kinase pim-1(PIM1) ELISA kit 96T
UB-E05024 Human C-Raf Proto Oncogene Serine per Threonine Protein Kinase(CRAF)ELISA Kit 96T
E2796Hu Human C-Raf Proto Oncogene Serine per Threonine Protein Kinase (CRAF)ELISA Kit 48T
G6183 RAF proto-oncogene serine threonine-protein kinase (RAF1), Chicken, ELISA Kit 96T
YHB0856Hu Human C-Raf Proto Oncogene Serine-Threonine Protein Kinase (CRAF)ELISA Kit 48T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur