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RNA-directed RNA polymerase 2a (protein 2a) (EC 2.7.7.48)

 RDRP_CMVFN              Reviewed;         857 AA.
P16490;
01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
01-AUG-1990, sequence version 1.
15-MAR-2017, entry version 68.
RecName: Full=RNA-directed RNA polymerase 2a;
Short=protein 2a;
EC=2.7.7.48;
ORFNames=ORF2a;
Cucumber mosaic virus (strain FNY) (CMV).
Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage;
Bromoviridae; Cucumovirus.
NCBI_TaxID=12307;
NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
NCBI_TaxID=4097; Nicotiana tabacum (Common tobacco).
[1]
NUCLEOTIDE SEQUENCE [GENOMIC RNA].
PubMed=3404113; DOI=10.1099/0022-1317-69-8-1777;
Rizzo T.M., Palukaitis P.;
"Nucleotide sequence and evolutionary relationships of cucumber mosaic
virus (CMV) strains: CMV RNA 2.";
J. Gen. Virol. 69:1777-1787(1988).
-!- FUNCTION: RNA-dependent RNA polymerase which replicates the viral
genome composed of 3 RNA segments, RNA1, RNA2 and RNA3.
{ECO:0000305}.
-!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
+ RNA(n+1). {ECO:0000255|PROSITE-ProRule:PRU00539}.
-!- SUBUNIT: Interacts with replication protein 1a. {ECO:0000250}.
-!- SIMILARITY: Belongs to the ssRNA positive-strand viruses RNA-
directed RNA polymerase family. {ECO:0000305}.
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EMBL; D00355; BAA00263.1; -; Genomic_RNA.
PIR; JQ0015; WMVXCU.
RefSeq; NP_049324.1; NC_002035.1.
ProteinModelPortal; P16490; -.
PRIDE; P16490; -.
GeneID; 962642; -.
KEGG; vg:962642; -.
OrthoDB; VOG09000048; -.
Proteomes; UP000002502; Genome.
GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
GO; GO:0003723; F:RNA binding; IEA:InterPro.
GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
InterPro; IPR007094; RNA-dir_pol_PSvirus.
InterPro; IPR001788; Tymovirus_RNA-dep_RNA_pol.
Pfam; PF00978; RdRP_2; 1.
PROSITE; PS50507; RDRP_SSRNA_POS; 1.
3: Inferred from homology;
Complete proteome; Nucleotide-binding; Nucleotidyltransferase;
Reference proteome; RNA-directed RNA polymerase; Transferase;
Viral RNA replication.
CHAIN 1 857 RNA-directed RNA polymerase 2a.
/FTId=PRO_0000083273.
DOMAIN 511 624 RdRp catalytic. {ECO:0000255|PROSITE-
ProRule:PRU00539}.
SEQUENCE 857 AA; 96710 MW; 5BBA030FE68D7B2B CRC64;
MAFPAPAFSL ANLLNGSYGV DTPEDVERLR SEQREEAAAA CRNYRPLPAV DVSESVTEDA
HSLRTPDGAP AEAVSDEFVT YGAEDYLEKS DDELLVAFET MVKPMRIGQL WCPAFNKCSF
ISSIAMARAL LLAPRTSHRT MKCFEDLVAA IYTKSDFYYS EECEADDAQI DISSRDVPGY
SFEPWSRTSG FEPPPICEAC DMIMYQCPCF DFNALKKSCA ERTFADDYVI EGLDGVVDNA
TLLSNLGPFL VPVKCQYEKC PTPTIAIPPD LNRATDRVDI NLVQSICDST LPTHSNYDDS
FHQVFVESAD YSIDLDHVRL RQSDLIAKIP DSGHMIPVLN TGSGHKRVGT TKEVLTAIKK
RNADVPELGD SVNLSRLSKA VAERFFISYI NGNSLASSNF VNVVSNFHDY MEKWKSSGLS
YDDLPDLHAE NLQFYDHMIK SDVKPVVSDT LNIDRPVPAT ITYHKKSITS QFSPLFTALF
ERFQRCLRER IILPVGKISS LEMAGFDVKN KHCLEIDLSK FDKSQGEFHL LIQEHILNGL
GCPAPITKWW CDFHRFSYIR DRRAGVGMPI SFQRRTGDAL TYFGNTIVTM AEFAWCYDTD
QFEKLLFSGD DSLGFSLLPP VGDPSKFTTL FNMEAKVMEP AVPYICSKFL LSDEFGNTFS
VPDPLREVQR LGTKKIPYSD NDEFLFAHFM SFVDRLKFLD RMSQSCIDQL SIFFELKYKK
SGEEAALMLG AFKKYTANFQ SYKELYYSDR RQCELINSFC STEFRVERVN SNKQRKNYGI
ERRCNDKRRT PTGSYGGGEE AETKVSQTES TGTRSQKSQR ESAFKSQTIP LPTVLSSGWF
GTDRVMPPCE RGGVTRV


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