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RNA-directed RNA polymerase catalytic subunit (EC 2.7.7.48) (Polymerase basic protein 1) (PB1) (RNA-directed RNA polymerase subunit P1)

 RDRP_I33A0              Reviewed;         757 AA.
P03430;
21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
21-JUL-1986, sequence version 1.
30-AUG-2017, entry version 86.
RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000255|HAMAP-Rule:MF_04065};
EC=2.7.7.48 {ECO:0000255|HAMAP-Rule:MF_04065};
AltName: Full=Polymerase basic protein 1 {ECO:0000255|HAMAP-Rule:MF_04065};
Short=PB1 {ECO:0000255|HAMAP-Rule:MF_04065};
AltName: Full=RNA-directed RNA polymerase subunit P1 {ECO:0000255|HAMAP-Rule:MF_04065};
Name=PB1 {ECO:0000255|HAMAP-Rule:MF_04065};
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus
(strain A/WS/1933 H1N1)).
Viruses; ssRNA viruses; ssRNA negative-strand viruses;
Orthomyxoviridae; Influenzavirus A.
NCBI_TaxID=381518;
NCBI_TaxID=8782; Aves.
NCBI_TaxID=9606; Homo sapiens (Human).
NCBI_TaxID=9823; Sus scrofa (Pig).
[1]
NUCLEOTIDE SEQUENCE [GENOMIC RNA].
PubMed=7143569;
Sivasubramanian N., Nayak D.P.;
"Sequence analysis of the polymerase 1 gene and the secondary
structure prediction of polymerase 1 protein of human influenza virus
A/WSN/33.";
J. Virol. 44:321-329(1982).
[2]
NUCLEAR LOCALIZATION SIGNAL.
PubMed=2196448; DOI=10.1128/MCB.10.8.4139;
Nath S.T., Nayak D.P.;
"Function of two discrete regions is required for nuclear localization
of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1).";
Mol. Cell. Biol. 10:4139-4145(1990).
[3]
MUTAGENESIS OF ARG-233; LYS-235; ARG-238; ARG-239; ARG-249; PHE-251;
PHE-254; SER-269; VAL-273; LYS-278 AND LYS-281.
PubMed=16476991; DOI=10.1099/vir.0.81453-0;
Jung T.E., Brownlee G.G.;
"A new promoter-binding site in the PB1 subunit of the influenza A
virus polymerase.";
J. Gen. Virol. 87:679-688(2006).
-!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
replication and transcription of virus RNA segments. The
transcription of viral mRNAs occurs by a unique mechanism called
cap-snatching. 5' methylated caps of cellular mRNAs are cleaved
after 10-13 nucleotides by PA. In turn, these short capped RNAs
are used as primers by PB1 for transcription of viral mRNAs.
During virus replication, PB1 initiates RNA synthesis and copy
vRNA into complementary RNA (cRNA) which in turn serves as a
template for the production of more vRNAs. {ECO:0000255|HAMAP-
Rule:MF_04065}.
-!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
+ RNA(n+1). {ECO:0000255|HAMAP-Rule:MF_04065}.
-!- SUBUNIT: Influenza RNA polymerase is composed of three subunits:
PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-
terminus). Interacts (via C-terminus) with PB2 (via N-terminus);
this interaction is essential for transcription initiation.
{ECO:0000255|HAMAP-Rule:MF_04065}.
-!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000255|HAMAP-
Rule:MF_04065}. Host cytoplasm {ECO:0000255|HAMAP-Rule:MF_04065}.
-!- PTM: Phosphorylated by host PRKCA. {ECO:0000255|HAMAP-
Rule:MF_04065}.
-!- SIMILARITY: Belongs to the influenza viruses polymerase PB1
family. {ECO:0000255|HAMAP-Rule:MF_04065}.
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EMBL; J02178; AAA43582.1; ALT_SEQ; Genomic_RNA.
PIR; A04046; P1IV33.
SMR; P03430; -.
IntAct; P03430; 1.
MINT; MINT-8081772; -.
OrthoDB; VOG09000028; -.
GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW.
GO; GO:0039689; P:negative stranded viral RNA replication; IDA:UniProtKB.
GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-KW.
GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
HAMAP; MF_04065; INFV_RDRP; 1.
InterPro; IPR007099; RNA-dir_pol_NSvirus.
InterPro; IPR001407; RNA_pol_PB1_influenza.
Pfam; PF00602; Flu_PB1; 1.
PIRSF; PIRSF000827; RdRPol_OMV; 1.
PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
1: Evidence at protein level;
Eukaryotic host gene expression shutoff by virus;
Eukaryotic host transcription shutoff by virus; Host cytoplasm;
Host gene expression shutoff by virus; Host nucleus;
Host-virus interaction; Inhibition of host RNA polymerase II by virus;
Nucleotide-binding; Nucleotidyltransferase; Phosphoprotein;
RNA-directed RNA polymerase; Transferase; Viral RNA replication;
Viral transcription.
CHAIN 1 757 RNA-directed RNA polymerase catalytic
subunit.
/FTId=PRO_0000078767.
DOMAIN 286 483 RdRp catalytic. {ECO:0000255|HAMAP-
Rule:MF_04065}.
REGION 249 256 Promoter-binding site.
{ECO:0000255|HAMAP-Rule:MF_04065}.
MOTIF 187 195 Nuclear localization signal.
{ECO:0000255|HAMAP-Rule:MF_04065,
ECO:0000269|PubMed:2196448}.
MOTIF 203 216 Nuclear localization signal.
{ECO:0000255|HAMAP-Rule:MF_04065,
ECO:0000269|PubMed:2196448}.
MUTAGEN 233 233 R->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 235 235 K->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 238 238 R->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 239 239 R->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 249 249 R->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 251 251 F->A: 20% loss of RNA synthesis in vitro.
{ECO:0000269|PubMed:16476991}.
MUTAGEN 254 254 F->A: 50% loss of RNA synthesis in vitro.
{ECO:0000269|PubMed:16476991}.
MUTAGEN 269 269 S->A: 70% loss of RNA synthesis in vitro.
{ECO:0000269|PubMed:16476991}.
MUTAGEN 273 273 V->A: 80% loss of RNA synthesis in vitro.
{ECO:0000269|PubMed:16476991}.
MUTAGEN 278 278 K->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
MUTAGEN 281 281 K->A: Complete loss of RNA synthesis in
vitro. {ECO:0000269|PubMed:16476991}.
SEQUENCE 757 AA; 86534 MW; D5A0E8133CDE37F4 CRC64;
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS ERGRWTTNTE
TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFETSCLET MEVVQQTRVD
KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMNKEEME
ITTHFQRKRR VRDNMTKKMV TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA
IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK
SMKIRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG
VSILNLGQKR HTKTTYWWDG LQSSDDFALI VNAPNHEGIQ AGVNRFYRTC KLLGINMSKK
KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG
PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTHSKAGLL VSDGGPNLYN
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVNHKDIESV NNAVIMPAHG PAKNMEYDAV
ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS
RARIDARIDF ESGRIKKEEF TEIMKICSTI EELRRQK


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