GENTAUR Molecular Products.

 A0A0A8WQV6_9DELT        Unreviewed;       401 AA.
 A0A0A8WQV6;
 04-MAR-2015, integrated into UniProtKB/TrEMBL.
 04-MAR-2015, sequence version 1.
 16-JAN-2019, entry version 36.
 RecName: Full=Riboflavin biosynthesis protein RibBA {ECO:0000256|HAMAP-Rule:MF_01283};
 Includes:
   RecName: Full=3,4-dihydroxy-2-butanone 4-phosphate synthase {ECO:0000256|HAMAP-Rule:MF_01283};
            Short=DHBP synthase {ECO:0000256|HAMAP-Rule:MF_01283};
            EC=4.1.99.12 {ECO:0000256|HAMAP-Rule:MF_01283};
 Includes:
   RecName: Full=GTP cyclohydrolase-2 {ECO:0000256|HAMAP-Rule:MF_01283};
            EC=3.5.4.25 {ECO:0000256|HAMAP-Rule:MF_01283};
   AltName: Full=GTP cyclohydrolase II {ECO:0000256|HAMAP-Rule:MF_01283};
 Name=ribBA {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000313|EMBL:GAM10030.1};
 ORFNames=OR1_02316 {ECO:0000313|EMBL:GAM10030.1};
 Geobacter sp. OR-1.
 Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
 Geobacteraceae; Geobacter.
 NCBI_TaxID=1266765 {ECO:0000313|EMBL:GAM10030.1, ECO:0000313|Proteomes:UP000030972};
 [1] {ECO:0000313|EMBL:GAM10030.1, ECO:0000313|Proteomes:UP000030972}
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=OR-1 {ECO:0000313|EMBL:GAM10030.1,
 ECO:0000313|Proteomes:UP000030972};
 PubMed=23668621; DOI=10.1021/es400231x;
 Ohtsuka T., Yamaguchi N., Makino T., Sakurai K., Kimura K., Kudo K.,
 Homma E., Dong DT., Amachi S.;
 "Arsenic dissolution from Japanese paddy soil by a dissimilatory
 arsenate-reducing bacterium Geobacter sp. OR-1.";
 Environ. Sci. Technol. 47:6263-6271(2013).
 [2] {ECO:0000313|EMBL:GAM10030.1, ECO:0000313|Proteomes:UP000030972}
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=OR-1 {ECO:0000313|EMBL:GAM10030.1,
 ECO:0000313|Proteomes:UP000030972};
 Ehara A., Suzuki H., Amachi S.;
 "Draft Genome Sequence of Geobacter sp. Strain OR-1, an Arsenate-
 Respiring Bacterium Isolated from Japanese Paddy Soil.";
 Genome Announc. 3:e01478-14(2015).
 -!- FUNCTION: Catalyzes the conversion of D-ribulose 5-phosphate to
     formate and 3,4-dihydroxy-2-butanone 4-phosphate.
     {ECO:0000256|HAMAP-Rule:MF_01283, ECO:0000256|SAAS:SAAS00739193}.
 -!- FUNCTION: Catalyzes the conversion of GTP to 2,5-diamino-6-
     ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and
     pyrophosphate. {ECO:0000256|HAMAP-Rule:MF_01283}.
 -!- CATALYTIC ACTIVITY:
     Reaction=D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl
       phosphate + formate + H(+); Xref=Rhea:RHEA:18457,
       ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:58121,
       ChEBI:CHEBI:58830; EC=4.1.99.12; Evidence={ECO:0000256|HAMAP-
       Rule:MF_01283};
 -!- CATALYTIC ACTIVITY:
     Reaction=GTP + 3 H2O = 2,5-diamino-6-hydroxy-4-(5-
       phosphoribosylamino)-pyrimidine + diphosphate + formate + 2
       H(+); Xref=Rhea:RHEA:23704, ChEBI:CHEBI:15377,
       ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:33019,
       ChEBI:CHEBI:37565, ChEBI:CHEBI:58614; EC=3.5.4.25;
       Evidence={ECO:0000256|HAMAP-Rule:MF_01283,
       ECO:0000256|SAAS:SAAS01116086};
 -!- COFACTOR:
     Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
       Evidence={ECO:0000256|HAMAP-Rule:MF_01283};
     Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
       Evidence={ECO:0000256|HAMAP-Rule:MF_01283};
     Note=Binds 2 divalent metal cations per subunit. Magnesium or
     manganese. {ECO:0000256|HAMAP-Rule:MF_01283};
 -!- COFACTOR:
     Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
       Evidence={ECO:0000256|HAMAP-Rule:MF_01283};
     Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-
     Rule:MF_01283};
 -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 2-
     hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step
     1/1. {ECO:0000256|HAMAP-Rule:MF_01283}.
 -!- PATHWAY: Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-
     6-(D-ribitylamino)uracil from GTP: step 1/4. {ECO:0000256|HAMAP-
     Rule:MF_01283, ECO:0000256|SAAS:SAAS00711724}.
 -!- SIMILARITY: In the C-terminal section; belongs to the GTP
     cyclohydrolase II family. {ECO:0000256|HAMAP-Rule:MF_01283,
     ECO:0000256|SAAS:SAAS00789992}.
 -!- SIMILARITY: In the N-terminal section; belongs to the DHBP
     synthase family. {ECO:0000256|HAMAP-Rule:MF_01283,
     ECO:0000256|SAAS:SAAS00534513}.
 -!- CAUTION: Lacks conserved residue(s) required for the propagation
     of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01283}.
 -!- CAUTION: The sequence shown here is derived from an
     EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
     preliminary data. {ECO:0000313|EMBL:GAM10030.1}.
 -----------------------------------------------------------------------
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 EMBL; BAZF01000009; GAM10030.1; -; Genomic_DNA.
 RefSeq; WP_041971966.1; NZ_BAZF01000009.1.
 EnsemblBacteria; GAM10030; GAM10030; OR1_02316.
 OrthoDB; 900513at2; -.
 UniPathway; UPA00275; UER00399.
 UniPathway; UPA00275; UER00400.
 Proteomes; UP000030972; Unassembled WGS sequence.
 GO; GO:0008686; F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity; IEA:UniProtKB-UniRule.
 GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO; GO:0003935; F:GTP cyclohydrolase II activity; IEA:UniProtKB-UniRule.
 GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
 GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
 GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
 GO; GO:0009231; P:riboflavin biosynthetic process; IEA:UniProtKB-UniRule.
 CDD; cd00641; GTP_cyclohydro2; 1.
 Gene3D; 3.40.50.10990; -; 1.
 HAMAP; MF_00179; RibA; 1.
 HAMAP; MF_00180; RibB; 1.
 HAMAP; MF_01283; RibBA; 1.
 InterPro; IPR017945; DHBP_synth_RibB-like_a/b_dom.
 InterPro; IPR000422; DHBP_synthase_RibB.
 InterPro; IPR032677; GTP_cyclohydro_II.
 InterPro; IPR000926; RibA.
 InterPro; IPR036144; RibA-like_sf.
 InterPro; IPR016299; Riboflavin_synth_RibBA.
 Pfam; PF00926; DHBP_synthase; 1.
 Pfam; PF00925; GTP_cyclohydro2; 1.
 SUPFAM; SSF142695; SSF142695; 1.
 SUPFAM; SSF55821; SSF55821; 1.
 TIGRFAMs; TIGR00505; ribA; 1.
 TIGRFAMs; TIGR00506; ribB; 1.
 3: Inferred from homology;
 Complete proteome {ECO:0000313|Proteomes:UP000030972};
 GTP-binding {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000256|SAAS:SAAS00711691};
 Hydrolase {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000256|SAAS:SAAS01033620};
 Lyase {ECO:0000256|HAMAP-Rule:MF_01283};
 Magnesium {ECO:0000256|HAMAP-Rule:MF_01283};
 Manganese {ECO:0000256|HAMAP-Rule:MF_01283};
 Metal-binding {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000256|SAAS:SAAS00037896};
 Multifunctional enzyme {ECO:0000256|HAMAP-Rule:MF_01283};
 Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000256|SAAS:SAAS00711691};
 Reference proteome {ECO:0000313|Proteomes:UP000030972};
 Riboflavin biosynthesis {ECO:0000256|HAMAP-Rule:MF_01283,
 ECO:0000256|SAAS:SAAS00037880};
 Zinc {ECO:0000256|HAMAP-Rule:MF_01283, ECO:0000256|SAAS:SAAS00711685}.
 DOMAIN      208    373       GTP_cyclohydro2.
                              {ECO:0000259|Pfam:PF00925}.
 NP_BIND     253    257       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 NP_BIND     296    298       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 REGION        1    201       DHBP synthase. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 REGION       27     28       D-ribulose 5-phosphate binding.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 REGION      202    401       GTP cyclohydrolase II.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 ACT_SITE    330    330       Proton acceptor; for GTP cyclohydrolase
                              activity. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 ACT_SITE    332    332       Nucleophile; for GTP cyclohydrolase
                              activity. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 METAL        28     28       Magnesium or manganese 1.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 METAL        28     28       Magnesium or manganese 2.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 METAL       258    258       Zinc; catalytic. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 METAL       269    269       Zinc; catalytic. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 METAL       271    271       Zinc; catalytic. {ECO:0000256|HAMAP-
                              Rule:MF_01283}.
 BINDING      32     32       D-ribulose 5-phosphate.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 BINDING     164    164       D-ribulose 5-phosphate.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 BINDING     274    274       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 BINDING     318    318       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 BINDING     353    353       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 BINDING     358    358       GTP. {ECO:0000256|HAMAP-Rule:MF_01283}.
 SITE        126    126       Essential for DHBP synthase activity.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 SITE        164    164       Essential for DHBP synthase activity.
                              {ECO:0000256|HAMAP-Rule:MF_01283}.
 SEQUENCE   401 AA;  43922 MW;  62F21DD1EFE655CD CRC64;
 MPVSRIEDAI EDIRQGKMVI LVDDEDRENE GDLTMAAEKV TPEAINFMAR FGRGLICLSM
 TAERCDHLRL PLMVSTNTSS FGTAFTVSIE AKKGVTTGIS AADRAHTILT AVADDCKAED
 LARPGHVFPL RAKPGGVLVR AGQTEGSVDL ARLAGLKPAG VICEIMNDDG TMSRLPELRK
 FAKEHGIKIC TVADLVAYRL KHESLVRRAA EVKLPTSYGG DFKAIAFEND IDKLEHLALV
 KGEIHGDEPV LVRVHSECLT GDVFGSRRCD CCDQLHAAME MIDREGKGVV LYMRQEGRGI
 GLINKLKAYA LQDQGQDTVE ANLALGFKAD LRDYGIGAQI LVNLGVKKIR LMTNNPKKIV
 GLEGYGINIV ERVSIEVEPT KSNIKYLKTK RDKMGHLIEN I

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