Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Small archaeal modifier protein 2 (SAMP2) (Ubiquitin-like small archaeal modifier protein 2)

 SAMP2_HALVD             Reviewed;          66 AA.
D4GZE7;
10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
18-MAY-2010, sequence version 1.
28-FEB-2018, entry version 44.
RecName: Full=Small archaeal modifier protein 2;
Short=SAMP2;
AltName: Full=Ubiquitin-like small archaeal modifier protein 2;
Name=samp2; OrderedLocusNames=HVO_0202;
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC
14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
Archaea; Euryarchaeota; Halobacteria; Haloferacales; Haloferacaceae;
Haloferax.
NCBI_TaxID=309800;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 /
VKM B-1768 / DS2;
PubMed=20333302; DOI=10.1371/journal.pone.0009605;
Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S.,
Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M.,
Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T.,
Eisen J.A.;
"The complete genome sequence of Haloferax volcanii DS2, a model
archaeon.";
PLoS ONE 5:E9605-E9605(2010).
[2]
FUNCTION AS A PROTEIN MODIFIER, CROSS-LINK, PROTEIN TARGETS,
SAMPYLATION AT LYS-58, IDENTIFICATION BY MASS SPECTROMETRY, AND
MUTAGENESIS OF 65-GLY-GLY-66.
STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 /
VKM B-1768 / DS2;
PubMed=20054389; DOI=10.1038/nature08659;
Humbard M.A., Miranda H.V., Lim J.M., Krause D.J., Pritz J.R.,
Zhou G., Chen S., Wells L., Maupin-Furlow J.A.;
"Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax
volcanii.";
Nature 463:54-60(2010).
[3]
REVIEW.
PubMed=20547064; DOI=10.1016/j.tibs.2010.03.003;
Darwin K.H., Hofmann K.;
"SAMPyling proteins in archaea.";
Trends Biochem. Sci. 35:348-351(2010).
[4]
FUNCTION, THIOCARBOXYLATION AT GLY-66, AMPYLATION AT GLY-66, AND
DISRUPTION PHENOTYPE.
STRAIN=DS2 / DS70;
PubMed=21368171; DOI=10.1073/pnas.1018151108;
Miranda H.V., Nembhard N., Su D., Hepowit N., Krause D.J., Pritz J.R.,
Phillips C., Soll D., Maupin-Furlow J.A.;
"E1- and ubiquitin-like proteins provide a direct link between protein
conjugation and sulfur transfer in archaea.";
Proc. Natl. Acad. Sci. U.S.A. 108:4417-4422(2011).
[5]
FUNCTION.
STRAIN=DS2 / DS70;
PubMed=24097257; DOI=10.1074/mcp.M113.029652;
Miranda H.V., Antelmann H., Hepowit N., Chavarria N.E., Krause D.J.,
Pritz J.R., Basell K., Becher D., Humbard M.A., Brocchieri L.,
Maupin-Furlow J.A.;
"Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a
UbaA-dependent mechanism.";
Mol. Cell. Proteomics 13:220-239(2014).
[6]
STRUCTURE BY NMR.
Fan S., Zhang W., Liao S., Tu X.;
"Solution structure of ubiquitin-like small archaeal modifier protein
in Haloferax volcanii.";
Submitted (SEP-2010) to the PDB data bank.
[7]
STRUCTURE BY NMR.
Liao S., Zhang W., Fan K., Tu X.;
"Structure of a protein from Haloferax volcanii.";
Submitted (SEP-2011) to the PDB data bank.
[8]
X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS), SUBUNIT, AND MUTAGENESIS OF
GLU-53.
STRAIN=DS2;
PubMed=23821306; DOI=10.1002/pro.2305;
Li Y., Maciejewski M.W., Martin J., Jin K., Zhang Y.,
Maupin-Furlow J.A., Hao B.;
"Crystal structure of the ubiquitin-like small archaeal modifier
protein 2 from Haloferax volcanii.";
Protein Sci. 22:1206-1217(2013).
-!- FUNCTION: Functions as a protein modifier covalently attached to
lysine residues of substrate proteins, as well as a sulfur carrier
in tRNA thiolation. The protein modification process is termed
sampylation and involves the formation of an isopeptide bond
between the SAMP2 C-terminal glycine carboxylate and the epsilon-
amino group of lysine residues on target proteins. Is able to form
polymeric chains with itself at Lys-58, similar to ubiquitin and
other ubiquitin-like proteins. May serve as a proteolytic signal
in the cell to target proteins for degradation by proteasomes.
{ECO:0000269|PubMed:20054389, ECO:0000269|PubMed:21368171,
ECO:0000269|PubMed:24097257}.
-!- SUBUNIT: Monomer. {ECO:0000269|PubMed:23821306}.
-!- PTM: The C-terminal glycine is likely acyl-adenylated (-COAMP) by
UbaA, and also probably thiocarboxylated (-COSH) to function in
sulfur transfer. {ECO:0000269|PubMed:21368171}.
-!- DISRUPTION PHENOTYPE: Cells lacking this gene grow similarly to
wild-type in the presence of either DMSO or oxygen as the terminal
electron acceptor, but are retarded in aerobic growth at 50
degrees Celsius. The lysine tRNAs of the mutant strain appear to
be nonthiolated. {ECO:0000269|PubMed:21368171}.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
EMBL; CP001956; ADE03392.1; -; Genomic_DNA.
RefSeq; WP_004045316.1; NZ_AOHU01000106.1.
PDB; 2L32; NMR; -; A=1-66.
PDB; 2LJI; NMR; -; A=1-66.
PDB; 4HRS; X-ray; 2.30 A; A=2-66.
PDBsum; 2L32; -.
PDBsum; 2LJI; -.
PDBsum; 4HRS; -.
SMR; D4GZE7; -.
STRING; 309800.HVO_0202; -.
EnsemblBacteria; ADE03392; ADE03392; HVO_0202.
GeneID; 8926718; -.
KEGG; hvo:HVO_0202; -.
eggNOG; arCOG00535; Archaea.
eggNOG; COG2104; LUCA.
HOGENOM; HOG000227894; -.
KO; K03154; -.
OMA; MHVTVDV; -.
OrthoDB; POG093Z0OCI; -.
EvolutionaryTrace; D4GZE7; -.
Proteomes; UP000008243; Chromosome.
GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
GO; GO:0031386; F:protein tag; IDA:UniProtKB.
GO; GO:0032446; P:protein modification by small protein conjugation; IDA:UniProtKB.
InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
SUPFAM; SSF54285; SSF54285; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Isopeptide bond; Nucleotide-binding;
Phosphoprotein; Reference proteome; Ubl conjugation;
Ubl conjugation pathway.
CHAIN 1 66 Small archaeal modifier protein 2.
/FTId=PRO_0000397102.
MOD_RES 66 66 1-thioglycine; alternate.
{ECO:0000269|PubMed:21368171}.
MOD_RES 66 66 Glycyl adenylate; alternate.
{ECO:0000269|PubMed:21368171}.
CROSSLNK 58 58 Glycyl lysine isopeptide (Lys-Gly)
(interchain with G-Cter in SAMP2).
CROSSLNK 66 66 Glycyl lysine isopeptide (Gly-Lys)
(interchain with K-? in acceptor
proteins); alternate.
MUTAGEN 53 53 E->G: Abolishes SAMPylation of substrate
proteins. {ECO:0000269|PubMed:23821306}.
MUTAGEN 65 66 Missing: Abolishes SAMPylation of
substrate proteins.
{ECO:0000269|PubMed:20054389}.
STRAND 2 6 {ECO:0000244|PDB:4HRS}.
STRAND 12 16 {ECO:0000244|PDB:4HRS}.
HELIX 23 29 {ECO:0000244|PDB:4HRS}.
HELIX 34 36 {ECO:0000244|PDB:4HRS}.
STRAND 39 41 {ECO:0000244|PDB:4HRS}.
STRAND 44 46 {ECO:0000244|PDB:4HRS}.
HELIX 47 49 {ECO:0000244|PDB:2LJI}.
STRAND 50 53 {ECO:0000244|PDB:4HRS}.
STRAND 57 59 {ECO:0000244|PDB:2L32}.
SEQUENCE 66 AA; 7118 MW; 09442B0B845C7DE0 CRC64;
MNVTVEVVGE ETSEVAVDDD GTYADLVRAV DLSPHEVTVL VDGRPVPEDQ SVEVDRVKVL
RLIKGG


Related products :

Catalog number Product name Quantity
EIAAB40576 Homo sapiens,Human,Small ubiquitin-like protein 4,Small ubiquitin-related modifier 4,SMT3H4,SUMO4,SUMO-4
18-783-75567 RABBIT ANTI SUMO-1 (N-TERMINAL) - SMALL UBIQUITIN-RELATED MODIFIER-1; SUMO-1; Sentrin; Ubiquitin-like protein SMT3C; SMT3 homolog 3; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein UBL1 0.1 mg
E12399h Human Small Ubiquitin Related Modifier Protein 2 E 96T
E12400h Human Small Ubiquitin Related Modifier Protein 3 E 96T
E12401h Human Small Ubiquitin Related Modifier Protein 1 E 96T
H3634 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Pig, ELISA Kit 96T
LF-P0326 Sumo1, Human ( Small ubiquitin-related modifier), Protein 0.5 mg
AS08 308 rabbit polyclonal SUMO1 | small ubiquitin-like modifier protein 1 1.5 mg
Y050693 Anti-SUMO3(small ubiquitin-like modifier protein 3) Antibody 100ug
AS08 349 rabbit polyclonal SUMO3 | small ubiquitin-like modifier protein 3 50;
orb81400 Human Small Ubiquitin-Related Modifier 2 protein Proteins 10
orb81401 Human Small Ubiquitin-Related Modifier 2, GST protein Proteins 2
orb81399 Human Small Ubiquitin-Related Modifier 1 protein Proteins 10
E96152Hu ELISA Kit for Small Ubiquitin Related Modifier Protein 2 (SUMO2) 96T/Kit
UB-E00850 Human Small Ubiquitin Related Modifier Protein 2(SUMO2)ELISA Kit 96T
LF-P0326 Sumo1, Human ( Small ubiquitin-related modifier) type: Protein 0.5 mg
H3635 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Rabbit, ELISA Kit 96T
H3636 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Sheep, ELISA Kit 96T
E-EL-Ch0617 Chicken SUMO2 (Small Ubiquitin Related Modifier Protein 2) ELISA Kit 96T
E-EL-P2194 Porcine SUMO2 (Small Ubiquitin Related Modifier Protein 2) ELISA Kit 96T
H3633 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Monkey, ELISA Kit 96T
H3632 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Human, ELISA Kit 96T
H3631 SUMO2 (Small Ubiquitin Related Modifier Protein 2), Chicken, ELISA Kit 96T
201-12-4952 Human Small Ubiquitin Related Modifier Protein 2(SUMO2)ELISA Kit 96T
E-EL-MK0059 Monkey SUMO2 (Small Ubiquitin Related Modifier Protein 2) ELISA Kit 96T


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur