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Steroid 5-alpha-reductase DET2 (AtDET2) (EC 1.3.1.22) (Protein COMPACT ROSETTE 1) (Protein DEETIOLATED 2)

 DET2_ARATH              Reviewed;         262 AA.
Q38944; Q9SH83;
16-APR-2002, integrated into UniProtKB/Swiss-Prot.
16-APR-2002, sequence version 2.
12-SEP-2018, entry version 123.
RecName: Full=Steroid 5-alpha-reductase DET2;
Short=AtDET2;
EC=1.3.1.22;
AltName: Full=Protein COMPACT ROSETTE 1;
AltName: Full=Protein DEETIOLATED 2;
Name=DET2; Synonyms=CRO1; OrderedLocusNames=At2g38050;
ORFNames=T8P21.4;
Arabidopsis thaliana (Mouse-ear cress).
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
Arabidopsis.
NCBI_TaxID=3702;
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=8602526; DOI=10.1126/science.272.5260.398;
Li J., Nagpal P., Vitart V., McMorris T.C., Chory J.;
"A role for brassinosteroids in light-dependent development of
Arabidopsis.";
Science 272:398-401(1996).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
PubMed=10617197; DOI=10.1038/45471;
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis
thaliana.";
Nature 402:761-768(1999).
[3]
GENOME REANNOTATION.
STRAIN=cv. Columbia;
PubMed=27862469; DOI=10.1111/tpj.13415;
Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
Town C.D.;
"Araport11: a complete reannotation of the Arabidopsis thaliana
reference genome.";
Plant J. 89:789-804(2017).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
PubMed=14593172; DOI=10.1126/science.1088305;
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis
genome.";
Science 302:842-846(2003).
[5]
FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-204.
STRAIN=cv. Columbia;
PubMed=9401120; DOI=10.1105/tpc.9.11.1951;
Fujioka S., Li J., Choi Y.-H., Seto H., Takatsuto S., Noguchi T.,
Watanabe T., Kuriyama H., Yokota T., Chory J., Sakurai A.;
"The Arabidopsis deetiolated2 mutant is blocked early in
brassinosteroid biosynthesis.";
Plant Cell 9:1951-1962(1997).
[6]
FUNCTION, MUTAGENESIS OF GLU-204, CATALYTIC ACTIVITY,
BIOPHYSICOCHEMICAL PROPERTIES, AND ACTIVITY REGULATION.
STRAIN=cv. Columbia;
PubMed=9108014; DOI=10.1073/pnas.94.8.3554;
Li J., Biswas M.G., Chao A., Russell D.W., Chory J.;
"Conservation of function between mammalian and plant steroid 5alpha-
reductases.";
Proc. Natl. Acad. Sci. U.S.A. 94:3554-3559(1997).
[7]
DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-204.
PubMed=10394910; DOI=10.1007/s004380050016;
Serrano-Cartagena J., Robles P., Ponce M.R., Micol J.L.;
"Genetic analysis of leaf form mutants from the Arabidopsis
information service collection.";
Mol. Gen. Genet. 261:725-739(1999).
[8]
FUNCTION, MUTAGENESIS OF GLU-204, AND CATALYTIC ACTIVITY.
STRAIN=cv. Columbia;
PubMed=10398719; DOI=10.1104/pp.120.3.833;
Noguchi T., Fujioka S., Takatsuto S., Sakurai A., Yoshida S., Li J.,
Chory J.;
"Arabidopsis det2 is defective in the conversion of (24R)-24-
methylcholest-4-en-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in
brassinosteroid biosynthesis.";
Plant Physiol. 120:833-840(1999).
[9]
MUTAGENESIS OF GLU-204.
PubMed=11161033; DOI=10.1104/pp.125.2.763;
Steber C.M., McCourt P.;
"A role for brassinosteroids in germination in Arabidopsis.";
Plant Physiol. 125:763-769(2001).
[10]
FUNCTION, AND DISRUPTION PHENOTYPE.
STRAIN=cv. Columbia;
PubMed=11867704; DOI=10.1093/pcp/pcf024;
Nakaya M., Tsukaya H., Murakami N., Kato M.;
"Brassinosteroids control the proliferation of leaf cells of
Arabidopsis thaliana.";
Plant Cell Physiol. 43:239-244(2002).
[11]
FUNCTION, CATALYTIC ACTIVITY, AND MUTAGENESIS OF GLU-204.
STRAIN=cv. Columbia;
PubMed=15993049; DOI=10.1016/j.jsbmb.2005.04.035;
Rosati F., Bardazzi I., De Blasi P., Simi L., Scarpi D., Guarna A.,
Serio M., Racchi M.L., Danza G.;
"5alpha-Reductase activity in Lycopersicon esculentum: cloning and
functional characterization of LeDET2 and evidence of the presence of
two isoenzymes.";
J. Steroid Biochem. Mol. Biol. 96:287-299(2005).
-!- FUNCTION: Involved in a reduction step in the biosynthesis of the
plant steroid, brassinolide; acts at the second step in
brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-
methylcholest-4-en-3-one, which is further modified to form
campestanol. Can use progesterone, testosterone, androstenedione
and campestenone as substrate. {ECO:0000269|PubMed:10398719,
ECO:0000269|PubMed:11867704, ECO:0000269|PubMed:15993049,
ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9108014,
ECO:0000269|PubMed:9401120}.
-!- CATALYTIC ACTIVITY: A 3-oxo-5-alpha-steroid + NADP(+) = a 3-oxo-
Delta(4)-steroid + NADPH. {ECO:0000269|PubMed:10398719,
ECO:0000269|PubMed:15993049, ECO:0000269|PubMed:9108014}.
-!- ACTIVITY REGULATION: Inhibited by the 4-azasteroids 4-MA.
{ECO:0000269|PubMed:9108014}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=2.5 uM for testosterone {ECO:0000269|PubMed:9108014};
KM=0.4 uM for progesterone {ECO:0000269|PubMed:9108014};
Vmax=0.2 nmol/min/mg enzyme with testosterone as substrate
{ECO:0000269|PubMed:9108014};
Vmax=0.5 nmol/min/mg enzyme with progesterone as substrate
{ECO:0000269|PubMed:9108014};
pH dependence:
Optimum pH is 6.8. {ECO:0000269|PubMed:9108014};
-!- PATHWAY: Plant hormone biosynthesis; brassinosteroid biosynthesis.
-!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
protein {ECO:0000250}.
-!- DISRUPTION PHENOTYPE: Brassinolide-reversible dark- and light-
grown defects; small dark-green dwarf plants with a limited leaves
expension due to a reduced number of cells per leaf blade.
{ECO:0000269|PubMed:10394910, ECO:0000269|PubMed:11867704,
ECO:0000269|PubMed:8602526, ECO:0000269|PubMed:9401120}.
-!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
{ECO:0000305}.
-----------------------------------------------------------------------
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-----------------------------------------------------------------------
EMBL; U53860; AAC49264.1; -; mRNA.
EMBL; CP002685; AEC09484.1; -; Genomic_DNA.
EMBL; AY045926; AAK76600.1; -; mRNA.
EMBL; AY079337; AAL85068.1; -; mRNA.
PIR; C84800; C84800.
RefSeq; NP_181340.1; NM_129361.5.
UniGene; At.22739; -.
UniGene; At.66437; -.
ProteinModelPortal; Q38944; -.
BioGrid; 3727; 2.
IntAct; Q38944; 2.
STRING; 3702.AT2G38050.1; -.
PaxDb; Q38944; -.
EnsemblPlants; AT2G38050.1; AT2G38050.1; AT2G38050.
GeneID; 818383; -.
Gramene; AT2G38050.1; AT2G38050.1; AT2G38050.
KEGG; ath:AT2G38050; -.
Araport; AT2G38050; -.
TAIR; locus:2005506; AT2G38050.
eggNOG; KOG1638; Eukaryota.
eggNOG; ENOG410XS6P; LUCA.
HOGENOM; HOG000050133; -.
InParanoid; Q38944; -.
KO; K09591; -.
OMA; VEWCGFA; -.
OrthoDB; EOG09360J9S; -.
PhylomeDB; Q38944; -.
BioCyc; ARA:AT2G38050-MONOMER; -.
BioCyc; MetaCyc:AT2G38050-MONOMER; -.
UniPathway; UPA00381; -.
PRO; PR:Q38944; -.
Proteomes; UP000006548; Chromosome 2.
ExpressionAtlas; Q38944; baseline and differential.
Genevisible; Q38944; AT.
GO; GO:0005737; C:cytoplasm; IEA:InterPro.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IBA:GO_Central.
GO; GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:UniProtKB-EC.
GO; GO:0050213; F:progesterone 5-alpha-reductase activity; IDA:UniProtKB.
GO; GO:0009917; F:sterol 5-alpha reductase activity; IDA:TAIR.
GO; GO:0016132; P:brassinosteroid biosynthetic process; IDA:TAIR.
GO; GO:0010268; P:brassinosteroid homeostasis; IEP:TAIR.
GO; GO:0055114; P:oxidation-reduction process; IMP:UniProtKB.
InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
InterPro; IPR039357; SRD5A/TECR.
PANTHER; PTHR10556; PTHR10556; 1.
Pfam; PF02544; Steroid_dh; 1.
PIRSF; PIRSF015596; 5_alpha-SR2; 1.
PROSITE; PS50244; S5A_REDUCTASE; 1.
1: Evidence at protein level;
Brassinosteroid biosynthesis; Complete proteome; Lipid biosynthesis;
Lipid metabolism; Membrane; NADP; Oxidoreductase; Reference proteome;
Steroid biosynthesis; Transmembrane; Transmembrane helix.
CHAIN 1 262 Steroid 5-alpha-reductase DET2.
/FTId=PRO_0000213681.
TRANSMEM 13 33 Helical. {ECO:0000255}.
TRANSMEM 51 71 Helical. {ECO:0000255}.
TRANSMEM 79 99 Helical. {ECO:0000255}.
TRANSMEM 113 133 Helical. {ECO:0000255}.
TRANSMEM 148 168 Helical. {ECO:0000255}.
TRANSMEM 205 225 Helical. {ECO:0000255}.
MUTAGEN 204 204 E->K: In cro1-2 and det2-1; loss of
activity, enhanced sensitivity to
abscisic acid (ABA) during seed
germination.
{ECO:0000269|PubMed:10394910,
ECO:0000269|PubMed:10398719,
ECO:0000269|PubMed:11161033,
ECO:0000269|PubMed:15993049,
ECO:0000269|PubMed:9108014,
ECO:0000269|PubMed:9401120}.
CONFLICT 198 198 C -> R (in Ref. 1; AAC49264).
{ECO:0000305}.
SEQUENCE 262 AA; 30635 MW; 88291B8A8AF55664 CRC64;
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT
LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR LFRSSFPAGK NGFPITIAAL
AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE
NRGGYVIPRG GWFELVSCPN YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK
WYIAKFKEEY PKTRKAVIPF VY


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