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Tannase (EC 3.1.1.20) (Tannin acylhydrolase) (Fragment)

 TAN_BACIU               Reviewed;          15 AA.
C0HJL9;
01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
01-OCT-2014, sequence version 1.
11-MAY-2016, entry version 3.
RecName: Full=Tannase {ECO:0000303|Ref.2};
EC=3.1.1.20 {ECO:0000269|Ref.2};
AltName: Full=Tannin acylhydrolase {ECO:0000303|Ref.1};
Flags: Fragment;
Bacillus subtilis.
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
NCBI_TaxID=1423;
[1] {ECO:0000305}
PROTEIN SEQUENCE.
STRAIN=PAB2 {ECO:0000269|Ref.1};
Jana A., Mondal K.C., Das Mohapatra P.K.;
"Sequence insights of Tannase of Bacillus subtilis PAB2.";
Submitted (JUN-2014) to UniProtKB.
[2] {ECO:0000305}
FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL
PROPERTIES, AND SUBUNIT.
STRAIN=PAB2 {ECO:0000269|Ref.2};
DOI=10.1016/j.bej.2013.06.002;
Jana A., Maity C., Halder S.K., Das A., Pati B.R., Mondal K.C.,
Das Mohapatra P.K.;
"Structural characterization of thermostable, solvent tolerant,
cytosafe tannase from Bacillus subtilis PAB2.";
Biochem. Eng. J. 77:161-170(2013).
-!- FUNCTION: Hydrolyzes ester bonds of tannic acid to produce gallic
acid and glucose. Exhibits no cytotoxicity in vitro in Vero cells
or in vivo in a rat model. {ECO:0000269|Ref.2}.
-!- CATALYTIC ACTIVITY: Digallate + H(2)O = 2 gallate.
{ECO:0000269|Ref.2}.
-!- ENZYME REGULATION: Activity is enhanced by the addition of 1 mM
Mg(2+) (more than 100%) and by the addition of 1% glycerol (22%).
Activity is reduced by the addition of 1 mM of various metal ions,
including Hg(2+) (98%), Fe(3+) (95%), Fe(2+) (94%), Ba(2+) (92%),
Ca(2+) (76%), Co(2+) (70%), Zn(2+) (44%), Ag(2+) (14%) and K(+)
(8%). Inhibited by the addition of 1 mM DMSO (75%), Triton X-100
(42%), sodium azide (40%), beta-mercaptoethanol (34%), Tween 80
(22%), Tween 60 (20%), EDTA (18%) and phenylmethanesulfonyl
fluoride (PMSF) (7%) but not affected by the addition of SDS. Also
inhibited by the addition of 1% of acetic acid (73%), acetone
(67%) and butanol (40%) but not affected by the addition of
isopropanol, methanol and ethanol. {ECO:0000269|Ref.2}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=0.445 mM for tannic acid at 40 degrees Celsius and pH 5.0
{ECO:0000269|Ref.2};
Vmax=125.8 mM/min/mg enzyme with tannic acid as substrate
{ECO:0000269|Ref.2};
Note=Exhibits salt tolerance as it retains 67% of activity after
24 h exposure to 4 M NaCl. {ECO:0000269|Ref.2};
pH dependence:
Optimum pH is 5.0. Active from pH 3.0 to 7.0.
{ECO:0000269|Ref.2};
Temperature dependence:
Optimum temperature is 40 degrees Celsius. Retains 14% of its
maximal activity after heating 1 hour at 70 degrees Celsius.
{ECO:0000269|Ref.2};
-!- SUBUNIT: Monomer. {ECO:0000269|Ref.2}.
-!- SIMILARITY: Belongs to the tannase family.
{ECO:0000250|UniProtKB:P78581}.
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GO; GO:0050318; F:tannase activity; IEA:UniProtKB-EC.
1: Evidence at protein level;
Direct protein sequencing; Hydrolase; Serine esterase.
PEPTIDE 1 15 Tannase. {ECO:0000269|Ref.1}.
/FTId=PRO_0000430487.
NON_TER 15 15 {ECO:0000303|Ref.1}.
SEQUENCE 15 AA; 1798 MW; 319DF4660C905D26 CRC64;
VQPPHSHGDN FYIWT


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