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Tautomerase PptA (EC 5.3.2.-)

 PPTA_ECOLI              Reviewed;          77 AA.
P31992;
01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
23-JAN-2007, sequence version 3.
28-MAR-2018, entry version 133.
RecName: Full=Tautomerase PptA;
EC=5.3.2.-;
Name=pptA; Synonyms=ydcE; OrderedLocusNames=b1461, JW1456;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=8387990; DOI=10.1128/jb.175.10.2799-2808.1993;
Zhao S., Sandt C.H., Feulner G., Vlazny D.A., Gray J.A., Hill C.W.;
"Rhs elements of Escherichia coli K-12: complex composites of shared
and unique components that have different evolutionary histories.";
J. Bacteriol. 175:2799-2808(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=9097039; DOI=10.1093/dnares/3.6.363;
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M.,
Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K.,
Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N.,
Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J.,
Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome
corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF NATIVE PROTEIN AND COMPLEX
WITH INHIBITOR, FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL
PROPERTIES, SUBUNIT, AND MASS SPECTROMETRY.
STRAIN=K12;
PubMed=12356301; DOI=10.1021/bi020271h;
Almrud J.J., Kern A.D., Wang S.C., Czerwinski R.M., Johnson W.H. Jr.,
Murzin A.G., Hackert M.L., Whitman C.P.;
"The crystal structure of YdcE, a 4-oxalocrotonate tautomerase
homologue from Escherichia coli, confirms the structural basis for
oligomer diversity.";
Biochemistry 41:12010-12024(2002).
-!- FUNCTION: Can use enol isomers of phenylpyruvate, 2-hydroxy-2,4-
pentadienoate and (p-hydroxyphenyl)pyruvate as substrates.
{ECO:0000269|PubMed:12356301}.
-!- ENZYME REGULATION: Inhibited by (E)-2-fluoro-p-hydroxycinnamate.
{ECO:0000269|PubMed:12356301}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=205 uM for enol isomers of phenylpyruvate
{ECO:0000269|PubMed:12356301};
KM=220 uM for 2-hydroxy-2,4-pentadienoate
{ECO:0000269|PubMed:12356301};
KM=180 uM for (p-hydroxyphenyl)pyruvate
{ECO:0000269|PubMed:12356301};
-!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12356301}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
-!- MASS SPECTROMETRY: Mass=8540; Method=Electrospray; Range=2-77;
Evidence={ECO:0000269|PubMed:12356301};
-!- SIMILARITY: Belongs to the 4-oxalocrotonate tautomerase family.
PptA subfamily. {ECO:0000305}.
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EMBL; X60998; CAA43312.1; -; Genomic_DNA.
EMBL; U00096; AAC74543.1; -; Genomic_DNA.
EMBL; AP009048; BAA15092.1; -; Genomic_DNA.
PIR; H64898; H64898.
RefSeq; NP_415978.1; NC_000913.3.
RefSeq; WP_001120143.1; NZ_LN832404.1.
PDB; 1GYJ; X-ray; 2.10 A; A/B=2-77.
PDB; 1GYX; X-ray; 1.35 A; A/B=2-77.
PDB; 1GYY; X-ray; 1.35 A; A/B=2-77.
PDBsum; 1GYJ; -.
PDBsum; 1GYX; -.
PDBsum; 1GYY; -.
ProteinModelPortal; P31992; -.
SMR; P31992; -.
BioGrid; 4262891; 27.
IntAct; P31992; 14.
STRING; 316385.ECDH10B_1592; -.
DrugBank; DB02384; (E)-2-Fluoro-P-Hydroxycinnamate.
DrugBank; DB03793; Benzoic Acid.
PaxDb; P31992; -.
PRIDE; P31992; -.
EnsemblBacteria; AAC74543; AAC74543; b1461.
EnsemblBacteria; BAA15092; BAA15092; BAA15092.
GeneID; 945731; -.
KEGG; ecj:JW1456; -.
KEGG; eco:b1461; -.
PATRIC; fig|1411691.4.peg.807; -.
EchoBASE; EB1711; -.
EcoGene; EG11761; pptA.
eggNOG; ENOG4105KZK; Bacteria.
eggNOG; COG1942; LUCA.
HOGENOM; HOG000077848; -.
KO; K01821; -.
OMA; IKCFPRD; -.
BioCyc; EcoCyc:EG11761-MONOMER; -.
EvolutionaryTrace; P31992; -.
PRO; PR:P31992; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0016862; F:intramolecular oxidoreductase activity, interconverting keto- and enol-groups; IEA:InterPro.
GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro.
Gene3D; 3.30.429.10; -; 1.
HAMAP; MF_00718; Tautomerase_PptA; 1.
InterPro; IPR004370; 4-oxalocrotonate_tautomerase.
InterPro; IPR014347; Tautomerase/MIF_sf.
InterPro; IPR017284; Tautomerase_PptA.
Pfam; PF01361; Tautomerase; 1.
PIRSF; PIRSF037799; Tautomer_YdcE_prd; 1.
SUPFAM; SSF55331; SSF55331; 1.
1: Evidence at protein level;
3D-structure; Complete proteome; Cytoplasm; Isomerase;
Reference proteome.
INIT_MET 1 1 Removed.
CHAIN 2 77 Tautomerase PptA.
/FTId=PRO_0000209526.
ACT_SITE 2 2 Proton acceptor; via imino nitrogen.
STRAND 3 9 {ECO:0000244|PDB:1GYX}.
HELIX 15 33 {ECO:0000244|PDB:1GYX}.
HELIX 37 39 {ECO:0000244|PDB:1GYX}.
STRAND 41 46 {ECO:0000244|PDB:1GYX}.
HELIX 49 51 {ECO:0000244|PDB:1GYX}.
HELIX 52 58 {ECO:0000244|PDB:1GYX}.
TURN 59 66 {ECO:0000244|PDB:1GYX}.
SEQUENCE 77 AA; 8673 MW; D32FF4023FE556CA CRC64;
MPHIDIKCFP RELDEQQKAA LAADITDVII RHLNSKDSSI SIALQQIQPE SWQAIWDAEI
APQMEALIKK PGYSMNA


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