GENTAUR Molecular Products.

 BH063_ARATH             Reviewed;         335 AA.
 Q8GY61; O65678; Q8L851; Q8S3D9;
 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
 01-MAR-2003, sequence version 1.
 07-NOV-2018, entry version 109.
 RecName: Full=Transcription factor bHLH63;
 AltName: Full=Basic helix-loop-helix protein 63;
          Short=AtbHLH63;
          Short=bHLH 63;
 AltName: Full=Protein CRYPTOCHROME INTERACTING BASIC-HELIX-LOOP-HELIX 1;
 AltName: Full=Transcription factor EN 84;
 AltName: Full=bHLH transcription factor bHLH063;
 Name=BHLH63; Synonyms=CIB1, EN84; OrderedLocusNames=At4g34530;
 ORFNames=T4L20.110;
 Arabidopsis thaliana (Mouse-ear cress).
 Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
 Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
 Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
 Arabidopsis.
 NCBI_TaxID=3702;
 [1]
 NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
 NOMENCLATURE.
 STRAIN=cv. Columbia;
 PubMed=12679534; DOI=10.1093/molbev/msg088;
 Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
 "The basic helix-loop-helix transcription factor family in plants: a
 genome-wide study of protein structure and functional diversity.";
 Mol. Biol. Evol. 20:735-747(2003).
 [2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
 STRAIN=cv. Columbia;
 PubMed=10617198; DOI=10.1038/47134;
 Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
 Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
 Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
 Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
 Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
 Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
 Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
 Langham S.-A., McCullagh B., Bilham L., Robben J.,
 van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
 Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
 Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
 Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
 Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
 Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
 De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
 van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
 Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
 Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
 Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
 Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
 Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
 Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
 Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
 Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
 Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
 Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
 Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
 Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
 Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
 Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
 Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
 Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
 Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
 Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
 Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
 Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
 Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
 Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
 Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
 Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
 Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
 Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
 Chen E., Marra M.A., Martienssen R., McCombie W.R.;
 "Sequence and analysis of chromosome 4 of the plant Arabidopsis
 thaliana.";
 Nature 402:769-777(1999).
 [3]
 GENOME REANNOTATION.
 STRAIN=cv. Columbia;
 PubMed=27862469; DOI=10.1111/tpj.13415;
 Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
 Town C.D.;
 "Araport11: a complete reannotation of the Arabidopsis thaliana
 reference genome.";
 Plant J. 89:789-804(2017).
 [4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
 STRAIN=cv. Columbia;
 PubMed=11910074; DOI=10.1126/science.1071006;
 Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
 Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M.,
 Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T.,
 Shibata K., Shinagawa A., Shinozaki K.;
 "Functional annotation of a full-length Arabidopsis cDNA collection.";
 Science 296:141-145(2002).
 [5]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
 STRAIN=cv. Columbia;
 PubMed=14593172; DOI=10.1126/science.1088305;
 Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
 Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
 Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
 Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
 Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
 Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
 Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
 Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
 Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
 Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
 Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
 Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
 "Empirical analysis of transcriptional activity in the Arabidopsis
 genome.";
 Science 302:842-846(2003).
 [6]
 GENE FAMILY.
 PubMed=12897250; DOI=10.1105/tpc.013839;
 Toledo-Ortiz G., Huq E., Quail P.H.;
 "The Arabidopsis basic/helix-loop-helix transcription factor family.";
 Plant Cell 15:1749-1770(2003).
 [7]
 GENE FAMILY, AND NOMENCLATURE.
 PubMed=14600211; DOI=10.1105/tpc.151140;
 Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E.,
 Heim M.A., Jakoby M., Werber M., Weisshaar B.;
 "Update on the basic helix-loop-helix transcription factor gene family
 in Arabidopsis thaliana.";
 Plant Cell 15:2497-2502(2003).
 [8]
 FUNCTION, INTERACTION WITH CRY2, AND SUBCELLULAR LOCATION.
 PubMed=18988809; DOI=10.1126/science.1163927;
 Liu H., Yu X., Li K., Klejnot J., Yang H., Lisiero D., Lin C.;
 "Photoexcited CRY2 interacts with CIB1 to regulate transcription and
 floral initiation in Arabidopsis.";
 Science 322:1535-1539(2008).
 [9]
 INTERACTION WITH IBH1.
 PubMed=23161888; DOI=10.1105/tpc.112.105023;
 Ikeda M., Fujiwara S., Mitsuda N., Ohme-Takagi M.;
 "A triantagonistic basic helix-loop-helix system regulates cell
 elongation in Arabidopsis.";
 Plant Cell 24:4483-4497(2012).
 [10]
 BIOTECHNOLOGY.
 PubMed=22847441; DOI=10.1073/pnas.1211305109;
 Idevall-Hagren O., Dickson E.J., Hille B., Toomre D.K., De Camilli P.;
 "Optogenetic control of phosphoinositide metabolism.";
 Proc. Natl. Acad. Sci. U.S.A. 109:E2316-E2323(2012).
 [11]
 FUNCTION, AND INTERACTION WITH CRY2.
 PubMed=24130508; DOI=10.1371/journal.pgen.1003861;
 Liu Y., Li X., Li K., Liu H., Lin C.;
 "Multiple bHLH proteins form heterodimers to mediate CRY2-dependent
 regulation of flowering-time in Arabidopsis.";
 PLoS Genet. 9:E1003861-E1003861(2013).
 [12]
 INDUCTION BY BLUE LIGHT.
 STRAIN=cv. Columbia;
 PubMed=24101505; DOI=10.1073/pnas.1308987110;
 Liu H., Wang Q., Liu Y., Zhao X., Imaizumi T., Somers D.E.,
 Tobin E.M., Lin C.;
 "Arabidopsis CRY2 and ZTL mediate blue-light regulation of the
 transcription factor CIB1 by distinct mechanisms.";
 Proc. Natl. Acad. Sci. U.S.A. 110:17582-17587(2013).
 [13]
 INTERACTION WITH CRY2.
 PubMed=24780222; DOI=10.1016/j.ab.2014.04.023;
 Cui Y., Choudhury S.R., Irudayaraj J.;
 "Quantitative real-time kinetics of optogenetic proteins CRY2 and
 CIB1/N using single-molecule tools.";
 Anal. Biochem. 458:58-60(2014).
 [14]
 BIOTECHNOLOGY.
 PubMed=24718798; DOI=10.1007/978-1-4939-0470-9_8;
 Idevall-Hagren O., Decamilli P.;
 "Manipulation of plasma membrane phosphoinositides using photoinduced
 protein-protein interactions.";
 Methods Mol. Biol. 1148:109-128(2014).
 [15]
 BIOTECHNOLOGY.
 PubMed=25963241; DOI=10.1016/j.chembiol.2015.04.014;
 Duan L., Che D., Zhang K., Ong Q., Guo S., Cui B.;
 "Optogenetic control of molecular motors and organelle distributions
 in cells.";
 Chem. Biol. 22:671-682(2015).
 -!- FUNCTION: Transcription factor that binds DNA to G box 5'-CACGTG-
     3' and, to a lower extent, to E-box 5'-CANNTG-3' in vitro. Binds
     to chromatin DNA of the FT gene and promotes its expression, and
     thus triggers flowering in response to blue light.
     {ECO:0000269|PubMed:18988809, ECO:0000269|PubMed:24130508}.
 -!- SUBUNIT: Homodimer (Probable). Interacts with IBH1
     (PubMed:23161888). Binds reversibly to CRY2 after blue light
     illumination (PubMed:18988809, PubMed:24780222, PubMed:24130508).
     {ECO:0000269|PubMed:18988809, ECO:0000269|PubMed:23161888,
     ECO:0000269|PubMed:24130508, ECO:0000269|PubMed:24780222,
     ECO:0000305}.
 -!- INTERACTION:
     Q9C8Z9:BHLH148; NbExp=3; IntAct=EBI-4469930, EBI-4434374;
     Q96524:CRY2; NbExp=3; IntAct=EBI-4469930, EBI-531555;
     Q9SKX1:IBH1; NbExp=3; IntAct=EBI-4469930, EBI-4433589;
     Q9LXU1:PIM1; NbExp=3; IntAct=EBI-4469930, EBI-15193025;
 -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-
     ProRule:PRU00981, ECO:0000269|PubMed:18988809}.
 -!- TISSUE SPECIFICITY: Expressed constitutively in roots, leaves, and
     stems. {ECO:0000269|PubMed:12679534}.
 -!- INDUCTION: Accumulates strongly in response to blue light due to
     reduced preventing 26S proteasome-mediated degradation in an
     ADO1/ZTL and ADO2/LKP2 dependent manner, but levels decrease in
     the absence of blue light via 26S proteasome degradation (at
     protein level). {ECO:0000269|PubMed:24101505}.
 -!- BIOTECHNOLOGY: The blue light-mediated interaction between CRY2
     and BHLH63/CIB1 is used to design an optogenetic control of target
     proteins or organelles. {ECO:0000269|PubMed:22847441,
     ECO:0000269|PubMed:24718798, ECO:0000269|PubMed:25963241}.
 -!- SEQUENCE CAUTION:
     Sequence=CAA18832.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
     Sequence=CAB80170.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
 -----------------------------------------------------------------------
 Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
 Distributed under the Creative Commons Attribution (CC BY 4.0) License
 -----------------------------------------------------------------------
 EMBL; AF488596; AAM10952.1; -; mRNA.
 EMBL; AL023094; CAA18832.1; ALT_SEQ; Genomic_DNA.
 EMBL; AL161585; CAB80170.1; ALT_SEQ; Genomic_DNA.
 EMBL; CP002687; AEE86389.1; -; Genomic_DNA.
 EMBL; AK117846; BAC42487.1; -; mRNA.
 EMBL; AY120741; AAM53299.1; -; mRNA.
 EMBL; BT005313; AAO63377.1; -; mRNA.
 PIR; T05273; T05273.
 RefSeq; NP_195179.2; NM_119618.3.
 UniGene; At.48937; -.
 ProteinModelPortal; Q8GY61; -.
 SMR; Q8GY61; -.
 BioGrid; 14886; 6.
 DIP; DIP-59356N; -.
 IntAct; Q8GY61; 37.
 STRING; 3702.AT4G34530.1; -.
 PaxDb; Q8GY61; -.
 EnsemblPlants; AT4G34530.1; AT4G34530.1; AT4G34530.
 GeneID; 829604; -.
 Gramene; AT4G34530.1; AT4G34530.1; AT4G34530.
 KEGG; ath:AT4G34530; -.
 Araport; AT4G34530; -.
 TAIR; locus:2139484; AT4G34530.
 eggNOG; ENOG410IGTK; Eukaryota.
 eggNOG; ENOG410YGAM; LUCA.
 HOGENOM; HOG000238007; -.
 InParanoid; Q8GY61; -.
 OMA; GYSHEMV; -.
 OrthoDB; EOG09360J45; -.
 PhylomeDB; Q8GY61; -.
 PRO; PR:Q8GY61; -.
 Proteomes; UP000006548; Chromosome 4.
 GO; GO:0005634; C:nucleus; IDA:TAIR.
 GO; GO:0003677; F:DNA binding; IDA:TAIR.
 GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
 GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
 GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
 GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
 GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
 GO; GO:0009637; P:response to blue light; IDA:UniProtKB.
 GO; GO:0006351; P:transcription, DNA-templated; IDA:TAIR.
 CDD; cd00083; HLH; 1.
 Gene3D; 4.10.280.10; -; 1.
 InterPro; IPR011598; bHLH_dom.
 InterPro; IPR024097; bHLH_ZIP_TF.
 InterPro; IPR036638; HLH_DNA-bd_sf.
 PANTHER; PTHR12565; PTHR12565; 1.
 Pfam; PF00010; HLH; 1.
 SMART; SM00353; HLH; 1.
 SUPFAM; SSF47459; SSF47459; 1.
 PROSITE; PS50888; BHLH; 1.
 1: Evidence at protein level;
 Activator; Complete proteome; DNA-binding; Flowering; Nucleus;
 Reference proteome; Transcription; Transcription regulation.
 CHAIN         1    335       Transcription factor bHLH63.
                              /FTId=PRO_0000358758.
 DOMAIN      178    228       bHLH. {ECO:0000255|PROSITE-
                              ProRule:PRU00981}.
 CONFLICT     86     86       L -> F (in Ref. 5; AAM53299).
                              {ECO:0000305}.
 CONFLICT    110    110       M -> V (in Ref. 1; AAM10952).
                              {ECO:0000305}.
 SEQUENCE   335 AA;  37540 MW;  05F0CA14FC2B3C7A CRC64;
 MNGAIGGDLL LNFPDMSVLE RQRAHLKYLN PTFDSPLAGF FADSSMITGG EMDSYLSTAG
 LNLPMMYGET TVEGDSRLSI SPETTLGTGN FKKRKFDTET KDCNEKKKKM TMNRDDLVEE
 GEEEKSKITE QNNGSTKSIK KMKHKAKKEE NNFSNDSSKV TKELEKTDYI HVRARRGQAT
 DSHSIAERVR REKISERMKF LQDLVPGCDK ITGKAGMLDE IINYVQSLQR QIEFLSMKLA
 IVNPRPDFDM DDIFAKEVAS TPMTVVPSPE MVLSGYSHEM VHSGYSSEMV NSGYLHVNPM
 QQVNTSSDPL SCFNNGEAPS MWDSHVQNLY GNLGV

Related products :