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Translocase of chloroplast 34 (EC 3.6.5.-) (34 kDa chloroplast outer envelope protein) (GTP-binding protein IAP34) (GTP-binding protein OEP34)

 TOC34_PEA               Reviewed;         310 AA.
Q41009; Q41029;
15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-NOV-1996, sequence version 1.
25-OCT-2017, entry version 104.
RecName: Full=Translocase of chloroplast 34;
EC=3.6.5.-;
AltName: Full=34 kDa chloroplast outer envelope protein;
AltName: Full=GTP-binding protein IAP34;
AltName: Full=GTP-binding protein OEP34;
Name=TOC34; Synonyms=IAP34, OEP34;
Pisum sativum (Garden pea).
Plastid; Chloroplast.
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
Fabeae; Pisum.
NCBI_TaxID=3888;
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
STRAIN=cv. Miranda; TISSUE=Leaf;
PubMed=7757113; DOI=10.1046/j.1365-313X.1995.7030401.x;
Seedorf M., Waegemann K., Soll J.;
"A constituent of the chloroplast import complex represents a new type
of GTP-binding protein.";
Plant J. 7:401-411(1995).
[2]
NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 119-139 AND
157-180.
PubMed=7973656; DOI=10.1126/science.7973656;
Kessler F., Blobel G., Patel H.V., Schnell D.J.;
"Identification of two GTP-binding proteins in the chloroplast protein
import machinery.";
Science 266:1035-1039(1994).
[3]
X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-258 IN COMPLEX WITH GTP,
HOMODIMERIZATION, AND MUTAGENESIS OF ARG-128.
PubMed=11753431; DOI=10.1038/nsb744;
Sun Y.-J., Forouhar F., Li Hm H.-M., Tu S.-L., Yeh Y.-H., Kao S.,
Shr H.-L., Chou C.-C., Chen C., Hsiao C.-D.;
"Crystal structure of pea Toc34, a novel GTPase of the chloroplast
protein translocon.";
Nat. Struct. Biol. 9:95-100(2002).
-!- FUNCTION: GTPase involved in protein precursor import into
chloroplasts. Seems to recognize chloroplast-destined precursor
proteins and regulate their presentation to the translocation
channel through GTP hydrolysis.
-!- SUBUNIT: Homodimer and monomer. Part of the TOC core complex that
includes a protein for the specific recognition of transit
peptides surrounded by a ring composed of four proteins forming
translocation channels, and four to five GTP-binding proteins
providing energy. This core complex can interact with components
of the TIC complex to form a larger import complex. Chloroplastic
protein precursor such as prSS (precursor of the RuBisCO small
subunit) interacts with these complexes. The TOC complex contains
a specific subset of polar lipids such as
digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and
phosphatidylglycerol (PG). TOC34 interacts at least with TOC75.
{ECO:0000269|PubMed:11753431}.
-!- INTERACTION:
Self; NbExp=5; IntAct=EBI-638506, EBI-638506;
P48347:GRF10 (xeno); NbExp=2; IntAct=EBI-638506, EBI-1803304;
Q9MUK5:TOC64; NbExp=6; IntAct=EBI-638506, EBI-638487;
-!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane.
-!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-
like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase
family. TOC34 subfamily. {ECO:0000305}.
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EMBL; Z28341; CAA82196.1; -; mRNA.
EMBL; L36856; AAC25785.1; -; mRNA.
PIR; B55171; B55171.
PDB; 1H65; X-ray; 2.00 A; A/B/C=1-258.
PDB; 3BB1; X-ray; 2.80 A; A/B/C/D/E/F/G/H=1-266.
PDBsum; 1H65; -.
PDBsum; 3BB1; -.
ProteinModelPortal; Q41009; -.
SMR; Q41009; -.
DIP; DIP-332N; -.
IntAct; Q41009; 9.
MINT; MINT-2584005; -.
TCDB; 3.A.9.1.1; the chloroplast envelope protein translocase (cept or tic-toc) family.
PRIDE; Q41009; -.
BRENDA; 3.6.5.2; 4872.
EvolutionaryTrace; Q41009; -.
GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
GO; GO:0042802; F:identical protein binding; IPI:IntAct.
GO; GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro.
GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
InterPro; IPR006703; G_AIG1.
InterPro; IPR027417; P-loop_NTPase.
InterPro; IPR005688; Toc34.
Pfam; PF04548; AIG1; 1.
PIRSF; PIRSF038134; Toc34; 1.
SUPFAM; SSF52540; SSF52540; 1.
TIGRFAMs; TIGR00991; 3a0901s02IAP34; 1.
PROSITE; PS51720; G_AIG1; 1.
1: Evidence at protein level;
3D-structure; Chloroplast; Direct protein sequencing; GTP-binding;
Hydrolase; Membrane; Nucleotide-binding; Plastid;
Plastid outer membrane; Protein transport; Transmembrane;
Transmembrane helix; Transport.
CHAIN 1 310 Translocase of chloroplast 34.
/FTId=PRO_0000144793.
TOPO_DOM 1 268 Cytoplasmic. {ECO:0000255}.
TRANSMEM 269 289 Helical. {ECO:0000255}.
TOPO_DOM 290 310 Chloroplast intermembrane. {ECO:0000255}.
DOMAIN 37 261 AIG1-type G.
NP_BIND 49 54 GTP. {ECO:0000269|PubMed:11753431}.
NP_BIND 210 211 GTP. {ECO:0000269|PubMed:11753431}.
REGION 68 71 Homodimerization.
REGION 128 133 Homodimerization.
BINDING 73 73 GTP. {ECO:0000269|PubMed:11753431}.
MUTAGEN 128 128 R->A: No dimerization and reduced GTPase
activity. {ECO:0000269|PubMed:11753431}.
HELIX 12 16 {ECO:0000244|PDB:1H65}.
HELIX 19 34 {ECO:0000244|PDB:1H65}.
STRAND 39 47 {ECO:0000244|PDB:1H65}.
HELIX 52 60 {ECO:0000244|PDB:1H65}.
STRAND 69 71 {ECO:0000244|PDB:1H65}.
STRAND 78 84 {ECO:0000244|PDB:1H65}.
STRAND 87 93 {ECO:0000244|PDB:1H65}.
STRAND 97 99 {ECO:0000244|PDB:1H65}.
HELIX 105 114 {ECO:0000244|PDB:1H65}.
TURN 115 117 {ECO:0000244|PDB:1H65}.
STRAND 122 130 {ECO:0000244|PDB:1H65}.
HELIX 136 149 {ECO:0000244|PDB:1H65}.
HELIX 151 156 {ECO:0000244|PDB:1H65}.
STRAND 157 162 {ECO:0000244|PDB:1H65}.
HELIX 169 171 {ECO:0000244|PDB:1H65}.
HELIX 174 192 {ECO:0000244|PDB:1H65}.
HELIX 200 202 {ECO:0000244|PDB:1H65}.
STRAND 206 208 {ECO:0000244|PDB:1H65}.
HELIX 231 243 {ECO:0000244|PDB:1H65}.
STRAND 245 247 {ECO:0000244|PDB:1H65}.
HELIX 254 258 {ECO:0000244|PDB:1H65}.
SEQUENCE 310 AA; 34157 MW; 00D5E79735BF041F CRC64;
MASQQQTVRE WSGINTFAPA TQTKLLELLG NLKQEDVNSL TILVMGKGGV GKSSTVNSII
GERVVSISPF QSEGPRPVMV SRSRAGFTLN IIDTPGLIEG GYINDMALNI IKSFLLDKTI
DVLLYVDRLD AYRVDNLDKL VAKAITDSFG KGIWNKAIVA LTHAQFSPPD GLPYDEFFSK
RSEALLQVVR SGASLKKDAQ ASDIPVVLIE NSGRCNKNDS DEKVLPNGIA WIPHLVQTIT
EVALNKSESI FVDKNLIDGP NPNQRGKLWI PLIFALQYLF LAKPIEALIR RDIATETKPA
WETRDVGDRK


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