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Tyrosine recombinase XerC

 XERC_ECOLI              Reviewed;         298 AA.
P0A8P6; P22885; Q2M8B7;
07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
28-MAR-2018, entry version 102.
RecName: Full=Tyrosine recombinase XerC;
Name=xerC; OrderedLocusNames=b3811, JW3784;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-20.
STRAIN=K12;
PubMed=2254268; DOI=10.1128/jb.172.12.6973-6980.1990;
Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.;
"Recombination at ColE1 cer requires the Escherichia coli xerC gene
product, a member of the lambda integrase family of site-specific
recombinases.";
J. Bacteriol. 172:6973-6980(1990).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=1379743; DOI=10.1126/science.1379743;
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
"Analysis of the Escherichia coli genome: DNA sequence of the region
from 84.5 to 86.5 minutes.";
Science 257:771-778(1992).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION
TO 184-185.
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
CHARACTERIZATION, AND MUTAGENESIS OF TYR-275.
STRAIN=K12 / DS941;
PubMed=8402918; DOI=10.1016/0092-8674(93)80076-Q;
Blakely G., May G., McCulloch R., Arciszewska L.K., Burke M.,
Lovett S.T., Sherratt D.J.;
"Two related recombinases are required for site-specific recombination
at dif and cer in E. coli K12.";
Cell 75:351-361(1993).
[6]
FUNCTION, AND MUTAGENESIS OF ARG-243 AND TYR-275.
STRAIN=K12 / DS941;
PubMed=7744017;
Arciszewska L.K., Sherratt D.J.;
"Xer site-specific recombination in vitro.";
EMBO J. 14:2112-2120(1995).
[7]
FUNCTION, AND MUTAGENESIS OF TYR-275.
PubMed=9268326; DOI=10.1074/jbc.272.35.21927;
Cornet F., Hallet B., Sherratt D.J.;
"Xer recombination in Escherichia coli. Site-specific DNA
topoisomerase activity of the XerC and XerD recombinases.";
J. Biol. Chem. 272:21927-21931(1997).
[8]
INTERACTION WITH XERC.
STRAIN=K12 / DS941;
PubMed=10361305; DOI=10.1046/j.1365-2958.1999.01418.x;
Spiers A.J., Sherratt D.J.;
"C-terminal interactions between the XerC and XerD site-specific
recombinases.";
Mol. Microbiol. 32:1031-1042(1999).
[9]
FUNCTION.
PubMed=10037776; DOI=10.1074/jbc.274.10.6763;
Grainge I., Sherratt D.J.;
"Xer site-specific recombination. DNA strand rejoining by recombinase
XerC.";
J. Biol. Chem. 274:6763-6769(1999).
[10]
ENZYME REGULATION, AND MUTAGENESIS OF 252-GLU--SER-254.
STRAIN=K12 / DS941;
PubMed=10635320; DOI=10.1016/S1097-2765(00)80224-5;
Hallet B., Arciszewska L.K., Sherratt D.J.;
"Reciprocal control of catalysis by the tyrosine recombinases XerC and
XerD: an enzymatic switch in site-specific recombination.";
Mol. Cell 4:949-959(1999).
[11]
ENZYME REGULATION BY FTSK.
PubMed=11832210; DOI=10.1016/S0092-8674(02)00624-4;
Aussel L., Barre F.-X., Aroyo M., Stasiak A., Stasiak A.Z.,
Sherratt D.J.;
"FtsK is a DNA motor protein that activates chromosome dimer
resolution by switching the catalytic state of the XerC and XerD
recombinases.";
Cell 108:195-205(2002).
-!- FUNCTION: Site-specific tyrosine recombinase, which acts by
catalyzing the cutting and rejoining of the recombining DNA
molecules. Binds cooperatively to specific DNA consensus sequences
that are separated from XerD binding sites by a short central
region, forming the heterotetrameric XerC-XerD complex that
recombines DNA substrates. The complex is essential to convert
dimers of the bacterial chromosome into monomers to permit their
segregation at cell division. It also contributes to the
segregational stability of plasmids at ColE1 xer (or cer) and
pSC101 (or psi) sites. In the complex XerC specifically exchanges
the top DNA strands (By similarity). {ECO:0000250,
ECO:0000269|PubMed:10037776, ECO:0000269|PubMed:7744017,
ECO:0000269|PubMed:9268326}.
-!- ENZYME REGULATION: During recombination, the heterotetrameric
complex catalyzes two consecutive pairs of strand exchanges,
implying that specific pairs of active sites are sequentially
switched on and off in the recombinase tetramer to ensure that
appropriate DNA strands will be exchanged at both reaction steps.
FtsK plays a central role in this catalytic state switch that
turns recombinase on and off reciprocally. The reciprocal C-
terminal interaction between XerC and XerD may also participate in
the enzymatic switch process. {ECO:0000269|PubMed:10635320,
ECO:0000269|PubMed:11832210}.
-!- SUBUNIT: Forms a cyclic heterotetrameric complex composed of two
molecules of XerC and two molecules of XerD, in which XerC
interacts with XerD via its C-terminal region, XerD interacts with
XerC via its C-terminal region and so on.
{ECO:0000269|PubMed:10361305}.
-!- INTERACTION:
P0A9E0:araC; NbExp=3; IntAct=EBI-1133806, EBI-1113479;
P77551:rzpR; NbExp=2; IntAct=EBI-1133806, EBI-9135212;
-!- SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with DNA.
-!- SIMILARITY: Belongs to the 'phage' integrase family. XerC
subfamily. {ECO:0000305}.
-----------------------------------------------------------------------
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EMBL; M38257; AAA24763.1; -; Genomic_DNA.
EMBL; M87049; AAA67607.1; -; Genomic_DNA.
EMBL; U00096; AAC76814.1; -; Genomic_DNA.
EMBL; AP009048; BAE77489.1; -; Genomic_DNA.
PIR; C37841; C37841.
RefSeq; NP_418256.1; NC_000913.3.
RefSeq; WP_000130691.1; NZ_LN832404.1.
PDB; 5DCF; X-ray; 2.30 A; A=111-298.
PDBsum; 5DCF; -.
ProteinModelPortal; P0A8P6; -.
SMR; P0A8P6; -.
BioGrid; 4263112; 411.
DIP; DIP-726N; -.
IntAct; P0A8P6; 9.
STRING; 316385.ECDH10B_4003; -.
PaxDb; P0A8P6; -.
PRIDE; P0A8P6; -.
EnsemblBacteria; AAC76814; AAC76814; b3811.
EnsemblBacteria; BAE77489; BAE77489; BAE77489.
GeneID; 948355; -.
KEGG; ecj:JW3784; -.
KEGG; eco:b3811; -.
PATRIC; fig|1411691.4.peg.2896; -.
EchoBASE; EB1062; -.
EcoGene; EG11069; xerC.
eggNOG; ENOG4108IYS; Bacteria.
eggNOG; COG4973; LUCA.
HOGENOM; HOG000045294; -.
InParanoid; P0A8P6; -.
KO; K03733; -.
OMA; FASHMLE; -.
PhylomeDB; P0A8P6; -.
BioCyc; EcoCyc:EG11069-MONOMER; -.
PRO; PR:P0A8P6; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0048476; C:Holliday junction resolvase complex; IDA:EcoliWiki.
GO; GO:0003677; F:DNA binding; IDA:EcoliWiki.
GO; GO:0009009; F:site-specific recombinase activity; IDA:EcoliWiki.
GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IDA:EcoliWiki.
GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
GO; GO:0006276; P:plasmid maintenance; IMP:EcoliWiki.
GO; GO:0042150; P:plasmid recombination; IMP:EcoliWiki.
GO; GO:0071139; P:resolution of recombination intermediates; IDA:EcoliWiki.
Gene3D; 1.10.150.130; -; 1.
Gene3D; 1.10.443.10; -; 1.
HAMAP; MF_01808; Recomb_XerC_XerD; 1.
InterPro; IPR011010; DNA_brk_join_enz.
InterPro; IPR013762; Integrase-like_cat_sf.
InterPro; IPR002104; Integrase_catalytic.
InterPro; IPR010998; Integrase_recombinase_N.
InterPro; IPR004107; Integrase_SAM-like_N.
InterPro; IPR011931; Recomb_XerC.
InterPro; IPR023009; Tyrosine_recombinase_XerC/XerD.
Pfam; PF02899; Phage_int_SAM_1; 1.
Pfam; PF00589; Phage_integrase; 1.
SUPFAM; SSF56349; SSF56349; 1.
TIGRFAMs; TIGR02224; recomb_XerC; 1.
1: Evidence at protein level;
3D-structure; Cell cycle; Cell division; Chromosome partition;
Complete proteome; Cytoplasm; Direct protein sequencing;
DNA integration; DNA recombination; DNA-binding; Reference proteome.
CHAIN 1 298 Tyrosine recombinase XerC.
/FTId=PRO_0000095294.
ACT_SITE 148 148 {ECO:0000250}.
ACT_SITE 172 172 {ECO:0000250}.
ACT_SITE 240 240 {ECO:0000250}.
ACT_SITE 243 243 {ECO:0000250}.
ACT_SITE 266 266 {ECO:0000250}.
ACT_SITE 275 275 O-(3'-phospho-DNA)-tyrosine intermediate.
MUTAGEN 243 243 R->Q: Abolishes DNA cleavage activity.
{ECO:0000269|PubMed:7744017}.
MUTAGEN 252 254 ESS->NHG: Abolishes chromosomal
recombination but not plasmid resolution.
{ECO:0000269|PubMed:10635320}.
MUTAGEN 275 275 Y->F: Abolishes DNA cleavage activity.
{ECO:0000269|PubMed:7744017,
ECO:0000269|PubMed:8402918,
ECO:0000269|PubMed:9268326}.
CONFLICT 184 185 NA -> KP (in Ref. 2; AAA67607).
{ECO:0000305}.
SEQUENCE 298 AA; 33868 MW; 91706E6945752FBA CRC64;
MTDLHTDVER YLRYLSVERQ LSPITLLNYQ RQLEAIINFA SENGLQSWQQ CDVTMVRNFA
VRSRRKGLGA ASLALRLSAL RSFFDWLVSQ NELKANPAKG VSAPKAPRHL PKNIDVDDMN
RLLDIDINDP LAVRDRAMLE VMYGAGLRLS ELVGLDIKHL DLESGEVWVM GKGSKERRLP
IGRNAVAWIE HWLDLRDLFG SEDDALFLSK LGKRISARNV QKRFAEWGIK QGLNNHVHPH
KLRHSFATHM LESSGDLRGV QELLGHANLS TTQIYTHLDF QHLASVYDAA HPRAKRGK


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