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Uric acid transporter UacT

 UACT_ECOLI              Reviewed;         482 AA.
Q46821; Q2M9V3;
01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
30-MAY-2000, sequence version 2.
05-JUL-2017, entry version 121.
RecName: Full=Uric acid transporter UacT;
Name=uacT; Synonyms=ygfU; OrderedLocusNames=b2888, JW5470;
Escherichia coli (strain K12).
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=83333;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=9278503; DOI=10.1126/science.277.5331.1453;
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1462(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
PubMed=16738553; DOI=10.1038/msb4100049;
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains
MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[3]
TOPOLOGY [LARGE SCALE ANALYSIS].
STRAIN=K12 / MG1655 / ATCC 47076;
PubMed=15919996; DOI=10.1126/science.1109730;
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
"Global topology analysis of the Escherichia coli inner membrane
proteome.";
Science 308:1321-1323(2005).
[4]
FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES,
SUBCELLULAR LOCATION, GENE NAME, AND MUTAGENESIS OF HIS-37; THR-100;
SER-256; THR-259; GLU-270; LEU-278; ASP-298; SER-317; GLN-318;
ASN-319; VAL-320; ARG-327 AND SER-426.
STRAIN=K12;
PubMed=22437829; DOI=10.1074/jbc.M112.355818;
Papakostas K., Frillingos S.;
"Substrate selectivity of YgfU, a uric acid transporter from
Escherichia coli.";
J. Biol. Chem. 287:15684-15695(2012).
-!- FUNCTION: Proton-dependent high-capacity transporter for uric
acid. Shows also a low capacity for transport of xanthine at 37
degrees Celsius but not at 25 degrees Celsius.
{ECO:0000269|PubMed:22437829}.
-!- ENZYME REGULATION: Inhibited in the presence of the protonophore
carbonyl cyanide m-chlorophenyl hydrazone.
{ECO:0000269|PubMed:22437829}.
-!- BIOPHYSICOCHEMICAL PROPERTIES:
Kinetic parameters:
KM=0.5 mM for uric acid {ECO:0000269|PubMed:22437829};
KM=0.3 mM for xanthine {ECO:0000269|PubMed:22437829};
Vmax=715 nmol/min/mg enzyme with acid uric as substrate
{ECO:0000269|PubMed:22437829};
Vmax=14 nmol/min/mg enzyme with xanthine as substrate (at 37
degrees Celsius) {ECO:0000269|PubMed:22437829};
-!- SUBCELLULAR LOCATION: Cell inner membrane
{ECO:0000269|PubMed:22437829}; Multi-pass membrane protein
{ECO:0000269|PubMed:22437829}.
-!- SIMILARITY: Belongs to the xanthine/uracil permease family.
Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily.
{ECO:0000305}.
-!- SEQUENCE CAUTION:
Sequence=AAA83069.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
-----------------------------------------------------------------------
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EMBL; U28375; AAA83069.1; ALT_INIT; Genomic_DNA.
EMBL; U00096; AAC75926.2; -; Genomic_DNA.
EMBL; AP009048; BAE76953.1; -; Genomic_DNA.
PIR; H65072; H65072.
RefSeq; NP_417364.2; NC_000913.3.
RefSeq; WP_001295374.1; NZ_LN832404.1.
ProteinModelPortal; Q46821; -.
BioGrid; 4262336; 160.
DIP; DIP-12176N; -.
STRING; 316385.ECDH10B_3062; -.
TCDB; 2.A.40.3.3; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
PaxDb; Q46821; -.
PRIDE; Q46821; -.
DNASU; 949017; -.
EnsemblBacteria; AAC75926; AAC75926; b2888.
EnsemblBacteria; BAE76953; BAE76953; BAE76953.
GeneID; 949017; -.
KEGG; ecj:JW5470; -.
KEGG; eco:b2888; -.
PATRIC; fig|1411691.4.peg.3847; -.
EchoBASE; EB2882; -.
EcoGene; EG13071; uacT.
eggNOG; ENOG4105C2W; Bacteria.
eggNOG; COG2233; LUCA.
HOGENOM; HOG000038199; -.
InParanoid; Q46821; -.
PhylomeDB; Q46821; -.
BioCyc; EcoCyc:YGFU-MONOMER; -.
BioCyc; MetaCyc:YGFU-MONOMER; -.
PRO; PR:Q46821; -.
Proteomes; UP000000318; Chromosome.
Proteomes; UP000000625; Chromosome.
GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
GO; GO:0015143; F:urate transmembrane transporter activity; IMP:EcoCyc.
GO; GO:0042907; F:xanthine transmembrane transporter activity; IMP:EcoCyc.
GO; GO:0015992; P:proton transport; IEA:UniProtKB-KW.
GO; GO:0015747; P:urate transport; IMP:EcoCyc.
GO; GO:0042906; P:xanthine transport; IMP:EcoCyc.
InterPro; IPR017588; UacT-like.
InterPro; IPR006042; Xan_ur_permease.
InterPro; IPR006043; Xant/urac/vitC.
Pfam; PF00860; Xan_ur_permease; 1.
TIGRFAMs; TIGR00801; ncs2; 1.
TIGRFAMs; TIGR03173; pbuX; 1.
PROSITE; PS01116; XANTH_URACIL_PERMASE; 1.
1: Evidence at protein level;
Cell inner membrane; Cell membrane; Complete proteome;
Hydrogen ion transport; Ion transport; Membrane; Reference proteome;
Symport; Transmembrane; Transmembrane helix; Transport.
CHAIN 1 482 Uric acid transporter UacT.
/FTId=PRO_0000165968.
TOPO_DOM 1 29 Cytoplasmic. {ECO:0000255}.
TRANSMEM 30 50 Helical. {ECO:0000255}.
TOPO_DOM 51 62 Periplasmic. {ECO:0000255}.
TRANSMEM 63 83 Helical. {ECO:0000255}.
TOPO_DOM 84 92 Cytoplasmic. {ECO:0000255}.
TRANSMEM 93 113 Helical. {ECO:0000255}.
TOPO_DOM 114 115 Periplasmic. {ECO:0000255}.
TRANSMEM 116 136 Helical. {ECO:0000255}.
TOPO_DOM 137 142 Cytoplasmic. {ECO:0000255}.
TRANSMEM 143 163 Helical. {ECO:0000255}.
TOPO_DOM 164 178 Periplasmic. {ECO:0000255}.
TRANSMEM 179 199 Helical. {ECO:0000255}.
TOPO_DOM 200 204 Cytoplasmic. {ECO:0000255}.
TRANSMEM 205 225 Helical. {ECO:0000255}.
TOPO_DOM 226 261 Periplasmic. {ECO:0000255}.
TRANSMEM 262 282 Helical. {ECO:0000255}.
TOPO_DOM 283 337 Cytoplasmic. {ECO:0000255}.
TRANSMEM 338 358 Helical. {ECO:0000255}.
TOPO_DOM 359 359 Periplasmic. {ECO:0000255}.
TRANSMEM 360 380 Helical. {ECO:0000255}.
TOPO_DOM 381 392 Cytoplasmic. {ECO:0000255}.
TRANSMEM 393 413 Helical. {ECO:0000255}.
TOPO_DOM 414 421 Periplasmic. {ECO:0000255}.
TRANSMEM 422 442 Helical. {ECO:0000255}.
TOPO_DOM 443 482 Cytoplasmic. {ECO:0000255}.
MUTAGEN 37 37 H->K,L: Lack of activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 37 37 H->N: Strong decrease in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 100 100 T->A: Decrease in activity with uric
acid. Highly active with xanthine.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 100 100 T->S: Decrease in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 256 256 S->Q: Almost no change in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 259 259 T->V: Almost no change in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 270 270 E->D,N,Q: Lack of activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 278 278 L->T: Almost no change in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 298 298 D->E: Strong decrease in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 298 298 D->N: Lack of activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 317 317 S->A: Decrease in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 318 318 Q->E,N: Strong decrease in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 319 319 N->D,Q: Lack of activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 320 320 V->N: Lack of activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 327 327 R->G: Almost no change in activity.
{ECO:0000269|PubMed:22437829}.
MUTAGEN 426 426 S->N: Almost no change in activity.
{ECO:0000269|PubMed:22437829}.
SEQUENCE 482 AA; 51758 MW; E08EF1D4CBD9D066 CRC64;
MSAIDSQLPS SSGQDRPTDE VDRILSPGKL IILGLQHVLV MYAGAVAVPL MIGDRLGLSK
EAIAMLISSD LFCCGIVTLL QCIGIGRFMG IRLPVIMSVT FAAVTPMIAI GMNPDIGLLG
IFGATIAAGF ITTLLAPLIG RLMPLFPPLV TGVVITSIGL SIIQVGIDWA AGGKGNPQYG
NPVYLGISFA VLIFILLITR YAKGFMSNVA VLLGIVFGFL LSWMMNEVNL SGLHDASWFA
IVTPMSFGMP IFDPVSILTM TAVLIIVFIE SMGMFLALGE IVGRKLSSHD IIRGLRVDGV
GTMIGGTFNS FPHTSFSQNV GLVSVTRVHS RWVCISSGII LILFGMVPKM AVLVASIPQF
VLGGAGLVMF GMVLATGIRI LSRCNYTTNR YNLYIVAISL GVGMTPTLSH DFFSKLPAVL
QPLLHSGIML ATLSAVVLNV FFNGYQHHAD LVKESVSDKD LKVRTVRMWL LMRKLKKNEH
GE


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