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tRNA sulfurtransferase (EC 2.8.1.4) (Sulfur carrier protein ThiS sulfurtransferase) (Thiamine biosynthesis protein ThiI) (tRNA 4-thiouridine synthase)

 A0A0A6VPM0_ECOLX        Unreviewed;       482 AA.
A0A0A6VPM0;
04-FEB-2015, integrated into UniProtKB/TrEMBL.
04-FEB-2015, sequence version 1.
22-NOV-2017, entry version 30.
RecName: Full=tRNA sulfurtransferase {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00898278};
EC=2.8.1.4 {ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00848765};
AltName: Full=Sulfur carrier protein ThiS sulfurtransferase {ECO:0000256|HAMAP-Rule:MF_00021};
AltName: Full=Thiamine biosynthesis protein ThiI {ECO:0000256|HAMAP-Rule:MF_00021};
AltName: Full=tRNA 4-thiouridine synthase {ECO:0000256|HAMAP-Rule:MF_00021};
Name=thiI {ECO:0000256|HAMAP-Rule:MF_00021};
ORFNames=AKN40_3769 {ECO:0000313|EMBL:ASO80543.1},
CES94_20250 {ECO:0000313|EMBL:ASG51015.1},
WR15_04200 {ECO:0000313|EMBL:KNF71440.1};
Escherichia coli.
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Escherichia.
NCBI_TaxID=562 {ECO:0000313|EMBL:KNF71440.1, ECO:0000313|Proteomes:UP000037564};
[1] {ECO:0000313|EMBL:KNF71440.1, ECO:0000313|Proteomes:UP000037564}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=CFSAN026843 {ECO:0000313|EMBL:KNF71440.1,
ECO:0000313|Proteomes:UP000037564};
Gonzalez-Escalona N., Toro M., Timme R., Payne J.;
"Genome sequences of 64 non-O157:H7 Shiga toxin-producing Escherichia
coli strains.";
Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
[2] {ECO:0000313|EMBL:ASO80543.1, ECO:0000313|Proteomes:UP000203621}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=MEM {ECO:0000313|EMBL:ASO80543.1,
ECO:0000313|Proteomes:UP000203621};
Liu B.;
"Domestication mutations in four distinct E. coli isolates are strain-
specific and environment dependent.";
Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
[3] {ECO:0000313|EMBL:ASG51015.1, ECO:0000313|Proteomes:UP000197584}
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ABWA45 {ECO:0000313|EMBL:ASG51015.1,
ECO:0000313|Proteomes:UP000197584};
Zurfluh K., Stephan R., Klumpp J., Hummerjohann J., Bagutti C.,
Marti R.;
"Genome of Escherichia coli isolate ABWA45, an rmtB encoding
wastewater isolate.";
Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
-!- FUNCTION: Catalyzes the ATP-dependent transfer of a sulfur to tRNA
to produce 4-thiouridine in position 8 of tRNAs, which functions
as a near-UV photosensor. Also catalyzes the transfer of sulfur to
the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate.
This is a step in the synthesis of thiazole, in the thiamine
biosynthesis pathway. The sulfur is donated as persulfide by IscS.
{ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00848766}.
-!- CATALYTIC ACTIVITY: ATP + [ThiI sulfur-carrier protein]-S-
sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-
sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI
sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-
sulfur] cluster. {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00860091}.
-!- CATALYTIC ACTIVITY: [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH +
[ThiS]-COSH + AMP. {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848762}.
-!- PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis.
{ECO:0000256|HAMAP-Rule:MF_00021, ECO:0000256|SAAS:SAAS00848764}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848759}.
-!- SIMILARITY: Belongs to the ThiI family. {ECO:0000256|HAMAP-
Rule:MF_00021, ECO:0000256|SAAS:SAAS00848754}.
-!- CAUTION: Lacks conserved residue(s) required for the propagation
of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00021}.
-----------------------------------------------------------------------
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EMBL; CP022154; ASG51015.1; -; Genomic_DNA.
EMBL; CP012378; ASO80543.1; -; Genomic_DNA.
EMBL; LGZN01000011; KNF71440.1; -; Genomic_DNA.
RefSeq; WP_000668689.1; NZ_NLUB01000007.1.
EnsemblBacteria; KHH09952; KHH09952; PU69_07855.
EnsemblBacteria; KIH37422; KIH37422; PD07_04490.
EnsemblBacteria; KLG77479; KLG77479; WR12_10985.
EnsemblBacteria; KNF71440; KNF71440; WR15_04200.
PATRIC; fig|562.7061.peg.2389; -.
UniPathway; UPA00060; -.
Proteomes; UP000037564; Unassembled WGS sequence.
Proteomes; UP000197584; Chromosome.
Proteomes; UP000203621; Chromosome.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
GO; GO:0016783; F:sulfurtransferase activity; IEA:UniProtKB-UniRule.
GO; GO:0004810; F:tRNA adenylyltransferase activity; IEA:InterPro.
GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-UniRule.
GO; GO:0009229; P:thiamine diphosphate biosynthetic process; IEA:UniProtKB-UniPathway.
GO; GO:0052837; P:thiazole biosynthetic process; IEA:InterPro.
GO; GO:0034227; P:tRNA thio-modification; IEA:UniProtKB-UniRule.
CDD; cd01712; ThiI; 1.
Gene3D; 3.40.250.10; -; 1.
Gene3D; 3.40.50.620; -; 1.
HAMAP; MF_00021; ThiI; 1.
InterPro; IPR001763; Rhodanese-like_dom.
InterPro; IPR036873; Rhodanese-like_dom_sf.
InterPro; IPR014729; Rossmann-like_a/b/a_fold.
InterPro; IPR026340; Thiazole_biosynth_dom.
InterPro; IPR020536; ThiI_AANH.
InterPro; IPR004114; THUMP_dom.
InterPro; IPR003720; tRNA_STrfase.
Pfam; PF02568; ThiI; 1.
Pfam; PF02926; THUMP; 1.
SMART; SM00981; THUMP; 1.
SUPFAM; SSF52821; SSF52821; 1.
TIGRFAMs; TIGR04271; ThiI_C_thiazole; 1.
TIGRFAMs; TIGR00342; TIGR00342; 1.
PROSITE; PS50206; RHODANESE_3; 1.
PROSITE; PS51165; THUMP; 1.
3: Inferred from homology;
ATP-binding {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00023005};
Complete proteome {ECO:0000313|Proteomes:UP000037564,
ECO:0000313|Proteomes:UP000197584, ECO:0000313|Proteomes:UP000203621};
Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848768};
Disulfide bond {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00898280};
Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00023005};
Redox-active center {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00898282};
RNA-binding {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848757};
Thiamine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848763};
Transferase {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848758, ECO:0000313|EMBL:KNF71440.1};
tRNA-binding {ECO:0000256|HAMAP-Rule:MF_00021,
ECO:0000256|SAAS:SAAS00848757}.
DOMAIN 61 165 THUMP. {ECO:0000259|PROSITE:PS51165}.
DOMAIN 404 482 Rhodanese. {ECO:0000259|PROSITE:PS50206}.
NP_BIND 183 184 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.
ACT_SITE 456 456 Cysteine persulfide intermediate.
{ECO:0000256|HAMAP-Rule:MF_00021}.
BINDING 265 265 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.
BINDING 287 287 ATP; via amide nitrogen.
{ECO:0000256|HAMAP-Rule:MF_00021}.
BINDING 296 296 ATP. {ECO:0000256|HAMAP-Rule:MF_00021}.
DISULFID 344 456 Redox-active. {ECO:0000256|HAMAP-
Rule:MF_00021}.
SEQUENCE 482 AA; 55001 MW; F33C4A648D9186AC CRC64;
MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI EVRAKDENQR
LAIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD QLEGKTFCVR VKRRGKHDFS
SIDVERYVGG GLNQHIESAR VKLTNPEVTV HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV
LSLISGGFDS GVSSYMLMRR GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV
AINFEPVVGE ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN
LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS PTVKAVKSKI
EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET VNGFGPNDVI LDIRSIDEQE
DNPLKVEGID VVSLPFYKLS TKFGDLDQNR TWLLWCERGV MSRLQALYLR EQGFNNVKVY
RP


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