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tRNA-specific 2-thiouridylase MnmA (EC 2.8.1.13)

 MNMA_FRATF              Reviewed;         359 AA.
A7N9A5;
15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
02-OCT-2007, sequence version 1.
05-JUL-2017, entry version 65.
RecName: Full=tRNA-specific 2-thiouridylase MnmA {ECO:0000255|HAMAP-Rule:MF_00144};
EC=2.8.1.13 {ECO:0000255|HAMAP-Rule:MF_00144};
Name=mnmA {ECO:0000255|HAMAP-Rule:MF_00144}; Synonyms=trmU;
OrderedLocusNames=FTA_0080;
Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA).
Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
Francisellaceae; Francisella.
NCBI_TaxID=458234;
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=FTNF002-00 / FTA;
PubMed=19756146; DOI=10.1371/journal.pone.0007041;
Barabote R.D., Xie G., Brettin T.S., Hinrichs S.H., Fey P.D.,
Jay J.J., Engle J.L., Godbole S.D., Noronha J.M., Scheuermann R.H.,
Zhou L.W., Lion C., Dempsey M.P.;
"Complete genome sequence of Francisella tularensis subspecies
holarctica FTNF002-00.";
PLoS ONE 4:E7041-E7041(2009).
-!- FUNCTION: Catalyzes the 2-thiolation of uridine at the wobble
position (U34) of tRNA, leading to the formation of s(2)U34.
{ECO:0000255|HAMAP-Rule:MF_00144}.
-!- CATALYTIC ACTIVITY: A [protein]-S-sulfanyl-L-cysteine +
uridine(34) in tRNA + ATP + reduced acceptor = a [protein]-L-
cysteine + 2-thiouridine(34) in tRNA + AMP + diphosphate +
acceptor. {ECO:0000255|HAMAP-Rule:MF_00144}.
-!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00144}.
-!- SIMILARITY: Belongs to the MnmA/TRMU family. {ECO:0000255|HAMAP-
Rule:MF_00144}.
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EMBL; CP000803; ABU60558.1; -; Genomic_DNA.
ProteinModelPortal; A7N9A5; -.
SMR; A7N9A5; -.
EnsemblBacteria; ABU60558; ABU60558; FTA_0080.
KEGG; fta:FTA_0080; -.
HOGENOM; HOG000218046; -.
KO; K00566; -.
OMA; AVCTGHY; -.
GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO; GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
CDD; cd01998; tRNA_Me_trans; 1.
Gene3D; 2.30.30.280; -; 1.
Gene3D; 3.40.50.620; -; 1.
HAMAP; MF_00144; tRNA_thiouridyl_MnmA; 1.
InterPro; IPR023382; Adenine_a_hdrlase_dom.
InterPro; IPR014729; Rossmann-like_a/b/a_fold.
InterPro; IPR004506; tRNA-specific_2-thiouridylase.
PANTHER; PTHR11933; PTHR11933; 1.
TIGRFAMs; TIGR00420; trmU; 1.
3: Inferred from homology;
ATP-binding; Cytoplasm; Disulfide bond; Nucleotide-binding;
RNA-binding; Transferase; tRNA processing; tRNA-binding.
CHAIN 1 359 tRNA-specific 2-thiouridylase MnmA.
/FTId=PRO_1000009524.
NP_BIND 9 16 ATP. {ECO:0000255|HAMAP-Rule:MF_00144}.
REGION 95 97 Interaction with target base in tRNA.
{ECO:0000255|HAMAP-Rule:MF_00144}.
REGION 147 149 Interaction with tRNA.
{ECO:0000255|HAMAP-Rule:MF_00144}.
REGION 309 310 Interaction with tRNA.
{ECO:0000255|HAMAP-Rule:MF_00144}.
ACT_SITE 100 100 Nucleophile. {ECO:0000255|HAMAP-
Rule:MF_00144}.
ACT_SITE 197 197 Cysteine persulfide intermediate.
{ECO:0000255|HAMAP-Rule:MF_00144}.
BINDING 35 35 ATP; via amide nitrogen and carbonyl
oxygen. {ECO:0000255|HAMAP-
Rule:MF_00144}.
BINDING 124 124 ATP; via amide nitrogen.
{ECO:0000255|HAMAP-Rule:MF_00144}.
SITE 125 125 Interaction with tRNA.
{ECO:0000255|HAMAP-Rule:MF_00144}.
SITE 342 342 Interaction with tRNA.
{ECO:0000255|HAMAP-Rule:MF_00144}.
DISULFID 100 197 Alternate. {ECO:0000255|HAMAP-
Rule:MF_00144}.
SEQUENCE 359 AA; 40130 MW; BED3FF9A6633DF43 CRC64;
MENKKVIVGI SGGVDSSVSA LLLKQQGYDV TGVFMKNWEE DDTDEFCSAE QDIADAQAVC
DSIGIPFKKI NFTAEYWDNV FEHFLIEYKA GRTPNPDILC NKEIKFKAFL SYVHLLGGDY
IATGHYAQTR LAADGSVQLV KGLDDNKDQT YFLYTLGQEQ LRQTIFPIGN IEKSKVREIA
KENNLVTFDK KDSTGICFIG ERKFKEFLSK YLPAQKGEIH DENGIKIGMH DGLMYYTIGQ
RQGLGIGGVK DRPEVPWFAA KKDLENNVLI AVQGHDHPLL FKQSLQAIEL SWVAGMAPAD
KFRCAAKVRY RQKDQSCEVE VNQDGSVNVT FDQPQRAITP GQSVVFYIDD VCLGGGVII


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