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Index / Ato / AB_2550 Kronos Dio (100V) / Product Detail : 3510120 AB_2550 Kronos Dio (100V)
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#3510120 AB_2550 Kronos Dio (100V)

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  Price : 35984   EUR
40840   USD
27912   GBP
151133   Zloty
4813798   JPY
277447   NOK
297289   SEK
40692   CHF

Product name : AB_2550 Kronos Dio (100V)

Catalog number : 3510120

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Supplier name : Ato

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A luminometer for real-time reporter assay with 8 dish incubator and multicolor measurement system


A luminescence monitoring tool to observe gene expressions with bioluminescent markers under long-term culture. Can be used in studies of internal biological clock, circadian rhythms in organisms, gene silencing, drug stimulation, etc. Kronos Dio is equiped with cell culture system within its housing to provide real-time periodical luminescence measurement with multi-color luciferase assay, enabling users to analyse the effects of complex factors influencing gene expression in the cell or the tissue. Internal temperature control (range: 20 to 45 °C) and carbon dioxide circulation allows for convenient cell culture over a long period of time.

 

http://www.laboproducts.com/prodct_Kronos.html

See also: Cellgraph AB-3000B

 

Product Number AB-2550
Product Name Kronos Dio
Container  Culture dish, diameter 35 mm
Number of samples  8
Temperature control  Peltier device and forced air circulation
Temperature range in Kronos Dio incubator   From room temperature – 5°C to 45°C  in increments of 1°C
(Lowest temp.; 20°C )
Temperature control accuracy in Kronos Dio incubator  ±0.5ºC  under room temperature of 25°C
Detector  Photomultiplier tube (PMT)
Measurement method Photon counting method with a photomultiplier tube
Detectable range of wavelength 350 nm to 670 nm
Measurement time  1 to 60 seconds in increments of 1 second
1 to 60 minutes in increments of 1 minute 
Measurement interval  From less than 1 minutes, which depends on time required for eight samples, to 300 minutes in increments of 1 minute
Optical long pass filter  F0: No filter  (whole wavelength)
F1: O56 filter  (cutoff wavelength: less than 560 nm ± 5 nm)
F2: R62 filter  (cutoff wavelength: less than 620 mm ± 5 nm)
Color separation Measurement with automatically selected optical filter up to three colors
CO 2 gas precision regulator  Maximum inlet pressure: 1 MPa
Pressure range: 0.007 to 0.07 MPa
CO 2 gas controller  Electromagnetic valve
Inlet pressure: -27 to 100 kPa
Outlet pressure: 0 to 50 kPa
CO 2 gas sensor  Measurement range: 0 to 20% CO 2   (±0.1%)
PC environmental requirements  OS: Windows XP/Vista/7  (32-bit version)
Recommended memory: more than 2GB
HD: Free space of 1 GB or more
Communication USB (Version 1.1) interface
Power AC100-230V 50/60Hz 110W
Dimensions 280(W) × 400(D) × 330(H) mm
Weight 16.0 kg

Literature

Published papers which refer to ATTO Kronos Dio are listed below.

Shin-ya Nishide, Sato Honma, Yoshihiro Nakajima, Masaaki Ikeda, Kenkiti Baba, Yoshihiro Ohmiya and Ken-ichi Honma, New reporter system for Per1 and Bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues. Genes to Cells 11, 1173-1182 (2006) 
Fujimori Sato, Masatoshi Kurokawa, Nobuhiko Yamauchi and Masa-aki Hattori, Gene silencing of myostatin in differential of chicken embryonic myoblasts by small interfering RNA. Am J Physiol Cell Physiol 291: C538-C545, 2006 
Hong Seok Shim, Hyunjung Kim, Jiwon Lee, Gi Hoon Son, Sehyung Cho, Tae H Oh, Sang Hyeon Kang, Dong-Seung Seen, Kun Ho Lee & Kyungjin Kim, Rapid activation of CLOCK by Ca2+-dependent protein kinase C mediates resetting of the mammalian circadian clock. EMBO reports 8, 4, 366-371 (2007) 
Tomoya Ohno,Yoshiaki Onishi, and Norio Ishida, A novel E4BP4 element drives circadian expression of mPeriod2. Nucleic Acids Res. 35(2): 648-655. (2007) [Nucleic Acids Research]
Hiroaki Fukuda, Pei Jian He, Kazuko Yokota, Tomoki Soh, Nobuhiko Yamauchi and Masa-aki Hattori, Progesterone-dependent and -independent expression of the multidrug resistance type I gene in porcine granulosa. Molecular and Cellular Biochemistry. Volume 298, Numbers 1-2, 179-186 (2007) 
Pei-Jian He, Masami Hirata, Nobuhiko Yamauchi, Seiichi Hashimoto and Masa-aki Hattori, Gonadotropic regulation of circadian clockwork in rat granulosa cells. Molecular and Cellular Biochemistry. Volume 302, Numbers 1-2, 111-118 (2007) 
He, Pei; Fujimoto, Yasunori; Yamauchi, Nobuhiko; Hattori, Masa-aki, Real-time monitoring of cAMP response element binding protein signaling in porcine granulosa cells modulated by ovarian factors. Molecular and Cellular Biochemistry.Volume 290, Numbers 1-2, 177-184 (2006) 

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