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Index / Ato / AB_3000B Cellgraph / Product Detail : 3600000 AB_3000B Cellgraph
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Nov
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#3600000 AB_3000B Cellgraph

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  Price : 134178   EUR
152285   USD
104082   GBP
563547   Zloty
17949756   JPY
1034550   NOK
1108536   SEK
151734   CHF

Product name : AB_3000B Cellgraph

Catalog number : 3600000

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Availability: Yes

Supplier name : Ato

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More Details about

Bioluminescnece imaging system for living cell and tissues 
  Cellgraph is an imaging system to detect low-level light emission in a single living cell by using a highly sensitive EM-CCD camera.

AB-3000B Cellgraph

AB-3000B Cellgraph

Purpose and Application
  • Real time reporter gene assay
  •   Transcriptional factor activity, Clock gene (chronopharmacology). Gene expression (Transfection,RNAi,etc)
  • Analysis of cell responses
  •   Drug response(Anti-cancer drug,DDS,etc),Stress response (Hormone,Inflammation,Anti-Oxidant), Cytotoxichology
  • Analysis of signal pathway
  • Signal cascade (Calcium), Apoptosis,etc
Features
  • Acquire images of culturing cells or tissue samples on a 35 mm dish in the living state.
  • Provide a CO 2 incubator equivalent atmosphere by controlling the temperature, CO 2 gas concentration and humidifying.
  • Highly sensitive and accurate detection of faint bioluminescence with an EM-CCD camera.
  • Isolates and captures multicolor faint bioluminescence.
  • Easy operation via a user-friendly interface.
  • Full control from a PC with dedicated software.
  • Completely lightproof,compact design.
Specifications
Product Number AB-3000B
Product Name Cellgraph
Cooled CCD Camera CCD type: Back-illuminated EMCCD
Active pixel: 512 x 512 pixel
Pixel size: 16 x 16μm
AD Resolution: 14/16 bit
Cooling temperature:
-80°C with an air-cooling system when the air temperature is 25°C, -90°C with a water-cooling system*
(when the water temperature is 25°C)
-85°C with an air-cooling system when the air temperature is 20°C, -100°C with a water-cooling system*
(when the water temperature is 16°C)
*The water-cooling unit is provided as an optional accessory.
Objective Lens 4x (NA 0.53) (Other magnification lenses are also available as optional accessories)
Stage X-Y-Z axis manual stage, Z-axis motorized stage
Culture dish 35mm culture dish
Constant temperature function (Room temperature +5°C) to 45°C, 0.1°C step
Lighting White LED with dimming function Blue LED with dimming function
Optical Filter Up to three filters can be set (525LP/560 LP/620 LP are standard equipment)
Exposure time 30 milliseconds to 90 minutes
Imaging interval Indefinite
Control software OS:Windows 7/Vista/XP
System requirement RAM: 1 GB or more (512 MB or more for XP)
  Hard Drive: 30 GB or more free disk space required
  Interface: Full size PCI slot x1, serial port x1, USB port x1
Power (main body) AC100-240V  106VA (varies according to the components of the whole system)
Dimensions (main body) 430 mm (W) x 600 mm (D) x 650 mm (H)
Weight (main body) Approx. 40kg
Optional Accessories
CCD camera water-cooling unit Constant-temperature water circulating system, heat-resistant tube for circulation
CO 2 gas injection unit CO 2 gas-mixer
CO 2 gas humidifying unit Bubbling system for gas humidification
Perfusion culture chamber unit Chamber for perfusion
Objective lenses 2x, 10x, 20x, 40x, 60x
References
H. Hoshino, Y. Nakajima and Y. Ohmiya, Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging. Nature Methods, 4
(8), 637-639 (2007)
C. Wu, K. Mino, H. Akimoto, M. Kawabata, K. Nakamura, M. Ozaki and Y. Ohmiya, In vivo far-red luminescence imaging of a biomarker based on
BRET from Cypridina bioluminescence to an organic dye. Proc. Natl. Acad. Sci. U.S.A., 106(37), 15599-15603 (2009)
Y. Nakajima, T. Yamazaki, S. Nishii, T. Noguchi, H. Hoshino, K. Niwa, V. R. Viviani and Y. Ohmiya, Enhanced beetle luciferase for high-resolution
bioluminescence imaging. PLoS One, 5(4), e10011 (2010)
H. Kwon, T. Enomoto, M. Shimogawara, K. Yasuda, Y. Nakajima and Y. Ohmiya, Bioluminescence imaging of dual gene expression at the single-cell
level. Biotechniques, 48(6), 460-462 (2010)
X. Li, Y. Nakajima, K. Niwa, VR. Viviani and Y. Ohmiya, Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging. Protein Sci.,
19, 26-33 (2010)
Y. Nakajima and Y. Ohmiya, Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay. Expert.
Opin. Drug Discov., 5(9), 835-849 (2010)
H. Kwon, Y. Ohmiya, K. Honma, S. Honma, T. Nagai, K. Saito and K. Yasuda, Synchronized ATP oscillations have a critical role in prechondrogenic
condensation during chondrogenesis. Cell Death Disease, 3. e278 (2012)

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