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#BIO-65048 MyTaq One_Step RT_PCR Kit

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Product name : MyTaq One_Step RT_PCR Kit

Catalog number : BIO-65048

Quantity: 25 Reactions

Availability: Yes

Supplier name : Biolin

Data sheet: Ask more or other datasheet now !

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More Details about

"When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch."

- The University of Adelaide, Australia

 

Features

 

• Extremely sensitive blend of RT and novel hot-start MyTaq
• Highly optimized for detection of low-copy genes
• Overcomes secondary structure in difficult and GC-rich targets
• High-quality, full-length cDNA from as little as 3pg of total RNA
• Simple to use all-in-one mix

 

Applications

 

• Gene-expression analysis
• Transcription analysis
• Gene cloning
• Multiplex RT-PCR

 

Description

MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube (Fig. 1), from either total RNA or mRNA using gene-specific primers. The kit contains the latest advances in buffer chemistry, including Bioline´s ultra-pure dNTPs, together with reverse transcriptase (RT) and our new generation of very high performance, antibody-mediated hot-start DNA polymerase (MyTaq HS). This ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultrasensitive products for downstream applications.

MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix.

The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-expression analysis.

The cDNA can be synthesised with starting amounts of RNA template from 3pg to 1µg, over a broad temperature range (up to 50°C (Fig. 2) to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS.
 

Components

 

• MyTaq One-Step Mix (2x)
• Ribosafe Inhibitor (10u/µl)
• Reverse transcriptase
• DEPC-treated Water

 

Storage Conditions

The MyTaq One-Step RT-PCR Kit can be stored for 6 months at -20°C.
 

Shipping Conditions

On Dry Ice or Blue Ice.
 

Product Citations

 

1. Buttermore, E.D., et al. J. Neurosci. 31(22), 8013-8024 (2011).

 

RT PCR kits of 100 reactions availabe HERE

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