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Index / ABM / to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region / Product Detail : Y051597 to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region
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Oct
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#Y051597 to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region

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Product name : to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region

Catalog number : Y051597

Quantity: 100μg

Availability: Yes

Supplier name : ABM

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More Details about

Antibody Type Primary Antibody
Description to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region is a The antibody is a rabbit polyclonal antibody made to the Type-II serine protease FAPa.. The antibody is made to a synthetic peptide based on the stalk region of human FAPa. The antibody reacts with human, mouse and rat FAPβ, and does not recognize other DPP family members. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. FAPβ (Fibroblast Activation Protein alpha subunit) is the alpha subunit and DPP-IV the beta subunit of a heterodimeric membrane-bound proteinase complex. Also known as 170 kDa Melanoma Membrane Gelatinase, Integral Membrane Serine Proteinase and Seprase, FAPβ was first identified as a membrane glycoprotein of fibroblasts and tumor cells, in a search for tumor cell markers. The beta subunit of the complex was identified as dipeptidyl peptidase-4 (DPP-IV), also known as CD26. Not all cells and tissues make the heterodimer; some make only FAPβ homodimers, some only DPP-4 homodimers. It has been reported that CD26/ FAPβ colocalizes with UPAR in melanoma cells, that larger complexes form with integrins and other connective tissue components, and that this impacts cell migration. Also reported is that FAPβ is produced by over 90% of human epithelial cancers, expressed by the stromal fibroblasts. FAPβ also is reported to promote tumor growth and angiogenesis in breast cancer model systems. FAPβ at the edges of invadipodia is thought to aid in cell migration and ECM/connective tissue breakdown. FAPβ is considered a dual-function proteinase, acting both as a dipeptidyl peptidase and as a collagenase, although there is some debate as to specificity. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa-Gly orientation. FAPβ is a member of a broader family of dipeptidyl peptidases including DPP-4, DPP-2, DPP-8, DPP-9, DPP-10, which have differing substrate specificities and tissue localizations. The surface-bound FAPβ is shed from cells, and cleavage of the stalk region releases the shed form found in serum. Two human FAPβ have been reported, 759 and 760 amino acids in length, with predicted mass of 87.7 and 87.8 kDa, and pI of 6.2 and 6.45 respectively. Postranslational modifications and reduction lead to an apparent mass of 110-120 kDa on SDS-PAGE gels. The 759 amino acid sequence has an insertion in the catalytic domain, but retains the catalytic triad. It is not clear what functional difference the two isoforms have. The FAPβ antibody recognizes both the 759 and 760 amino acid forms. There also is reported a much shorter sequence encoding only the carboxyterminal portion of FAPβ, only the catalytic domain. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
   
Immunogen Synthetic peptide of Human Fibroblast activation protein, alpha, corresponding to the stalk region.
Species Reactivity Human, Mouse, Rat
Specificity This antibody recognizes the Stalk Region of both long isoforms of Fibroblast activation protein, alpha but does not recognize other DPP family members.
Tested Applications Western Blot 1:1000
Application Notes Recommended dilutions WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Detects a band of approximately 110-120 kDa (predicted molecular weight: 88 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Raised In Rabbit
Clonality Polyclonal
Isotype IgG
Purity Immunogen Affinity purified
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Formulation Liquid
Concentration 1ug/ul
Storage The undiluted antibody solution is stable for 12 months at -20°C.
Caution to FAPβ (Fibroblast Activation Protein alpha subunit, Seprase, Integral Membrane Serine Protease) Stalk Region is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

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