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Index / Biolin / Tetro cDNA Synthesis Kit / Product Detail : BIO-65042 Tetro cDNA Synthesis Kit
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Sep
26th

#BIO-65042 Tetro cDNA Synthesis Kit

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  Price : 163   EUR
185   USD
126   GBP
687   Zloty
21900   JPY
1262   NOK
1352   SEK
185   CHF

Product name : Tetro cDNA Synthesis Kit

Catalog number : BIO-65042

Quantity: 30 Reactions

Availability: Yes

Supplier name : Biolin

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More Details about

Features & Benefits

  • Highly sensitive -for high-quality, full length cDNA from as little as 10pg of total RNA
  • Ultra-stable reverse transcriptase -for long genes and rare transcripts
  • Simple -one-tube, one-step set-up for highly sensitive RT-PCR
  • Broad dynamic range -10pg to 2μg of RNA

 

Applications

  • Construction of cDNA libraries
  • 2-step RT-PCR assays
  • Generation of probes for hybridization
  • Gene cloning

 

Description

The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers together with control RNA template and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as  analyses of cellular RNAs, characterization of RNA splice variants, and the generation and cloning of cDNA. 

The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10pg-2μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers together with control RNA template. The kit components are fully optimized to generate maximum yields of full-length cDNA.

 

Components

Reagent

30 reactions

100 reactions

5x RT Buffer

120µl

400µl

Reverse Transcriptase (200u/µl)

30µl

100µl

RNase Inhibitor (10u/µl)

30µl

100µl

dNTP Mix 10mM Total

30µl

100µl

Oligo (dT)18 Primer Mix

30µl

100µl

Random Hexamer Primer Mix

30µl

100µl

DEPC-treated Water

1.8ml

1.8ml


Storage & Stability

All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.


Shipping conditions

Tetro cDNA Synthesis Kit is shipped on dry/blue ice

 

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