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Index / Biolin / ISOLATE RNA Mini Kit / Product Detail : BIO-52042 ISOLATE RNA Mini Kit
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Jun
25th

#BIO-52042 ISOLATE RNA Mini Kit

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  Price : 64   EUR
72   USD
49   GBP
269   Zloty
8588   JPY
495   NOK
530   SEK
72   CHF

Product name : ISOLATE RNA Mini Kit

Catalog number : BIO-52042

Quantity: 10 preps

Availability: Yes

Supplier name : Biolin

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

ISOLATE RNA Mini Kit rna research rna is not so stable and very sticky. Rnases are abundand on hans and everywhere. SoreRNA at -75C and use rnase free.
http://gentaur-search.com/save_search/rna.htm

More Details about

 

TOTAL RNA ISOLATION FROM

ANIMAL TISSUE

1. Grind upto20mg sample using mortar and pestle with liquid N,. Transfer to 1.5ml tube. Add 450|jl Lysis Buffer R and homogenize. OR

1.     Place sample in suitable container. Add 450[jI Lysis Buffer R and homogenize using homogenizer. Transfer to 1.5ml tube.

2.     Spin: max. speed for 1 min.

3.     Transfer supernatant to Spin Column R1 placed in Collection Tube.

4.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

5.     Add 1 volume 70% ethanol to filtrate and mix well. Transfer to Spin Column R2 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

7.     Add 500pl Wash Buffer AR.

8.     Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

9.     Add 700[jl Wash Buffer BR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

12.   Add 30-80pi RNase-free water to Spin Column R2 membrane.

13.   Incubate at RT for 1 min.

14.   Spin: 6000 x g for 1 min. to elute RNA.

The isolated RNA is ready for use in downstream applications

 

TOTAL RNA ISOLATION FROM

PLANT TISSUE

1. Grind up to 100mg sample using mortar and pestle with liquid N2. Transfer to 1.5ml tube. Add 450pl Lysis Buffer APR or BPR and homogenize. OR

1.     Place sample in suitable container. Add 450^1 Lysis Buffer APR or BPR. Homogenize using homogenizer. Transfer to 1.5ml tube.

2.     Spin: max. speed for 1 min.

3.     Transfer supernatant to Spin Column PR1 placed in 2ml Collection Tube.

4.     Spin: 10,000 x g for 2 min. Discard Spin Column PR1 and SAVE THE FILTRATE.

5.     Add 1 volume of 70% ethanol to filtrate and mix well. Transfer to Spin Column PR2 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

7.     Add 500|jl Wash Buffer APR.

8.     Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

9.     Add 650|il Wash Buffer BPR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column PR2 in new Collection Tube.

11.   Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column PR2 in 1.5ml Elution Tube.

12.   Add 30-80pl RNase-free water directly to Spin Column PR2 membrane.

13.   Incubate at RT for 1 min.

14.   Spin: 6000 x g for 1 min. to elute RNA.

15.   The isolated RNA is ready for use in downstream applications.

TOTAL RNA ISOLATION FROM

EUKARYOTIC CELLS

1.     Harvest up to 5 x 10G cells.

Cells in suspension: Pellet cells at 300 x g for 5 min. Remove supernatant. Cells in monolayer: Remove cell culture medium.

2.     Add 400pl Lysis Buffer R.

3.     Resuspend pellet by pipetting.

4.     Incubate at RT for 3 min.

5.     Transfer supernatant to Spin Column R1 placed in Collection Tube.

6.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

7.     Add 1 volume 70% ethanol, mix well. Transfer to Spin Column R2 placed in new Collection Tube.

8.     Spin: 10,000 x g for 2 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

9.     Add 500pl Wash Buffer AR.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Add 700[il Wash Buffer BR.

12.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

13.   Spin: 10,000 x g for 3 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

14.   Add 30-80pl RNase-free water to Spin Column R2 membrane.

15.   Incubate at RT for 1 min.

16.   Spin: 6000 x g for 1 min. to elute RNA.

17.   The isolated RNA is ready for use in downstream applications.

 

 

TOTAL RNA ISOLATION FROM

BACTERIAL CELLS

1.     Pellet up to 1 x 10s cells at 5000 x g for 4 min. Discard supernatant.

2.     Resuspend cell pellet in 100^1 TE Buffer.

3.     Add 1pl to 6[jl lysozyme solution. Mix by pipetting.

4.     Add 450|jl Lysis Buffer R, vortex vigorously.

5.     Incubate at RT for 3 min.

6.     Transfer to Spin Column R1 placed in Collection Tube.

7.     Spin: 10,000 x g for 2 min. Discard Spin Column R1 and SAVE THE FILTRATE.

8.     Add 1 volume 70% ethanol to filtrate, mix well.

9.     Transfer 650|jl to Spin Column R2 placed in new Collection Tube.

10.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

11.   Transfer any remaining sample from step 9 to same Spin Column R2.

12.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

13.   Add 500pl Wash Buffer AR.

14.   Spin: 10,000 x g for 1 min. Discard Collection Tube, place Spin Column R2 in new Collection Tube.

15.   Add 700mI Wash Buffer BR.

16.   Repeat step 14.

17.   Spin: 10,000 x g for 3 min. Discard Collection Tube, place Spin Column R2 in 1.5ml Elution Tube.

18.   Add 30-80pi RNase-free water to Spin Column R2 membrane.

19.   Incubate at RT for 1 min.

20.   Spin: 6000 x g for 1 min. to elute RNA.

21.   The isolated RNA is ready for use in downstream applications.

 

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