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Index / ABM / to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody / Product Detail : Y051589 to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody
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Dec
18th

#Y051589 to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody

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  Price : 989   EUR
1123   USD
767   GBP
4156   Zloty
132404   JPY
7631   NOK
8177   SEK
1119   CHF

Product name : to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody

Catalog number : Y051589

Quantity: 100μg

Availability: Yes

Supplier name : ABM

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About this Product :

to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end Antibody.
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More Details about

Antibody Type Primary Antibody
Description to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end is a The antibody is a rabbit polyclonal antibody made to the serine protease DPP-8. The antibody is made to a synthetic peptide based on the aminoterminal end of human DPP-8. The antibody reacts with human, mouse and rat DPP-8, and does not recognize other DPP family members. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant. DPP-8 (Dipeptidyl Peptidase-8) is a serine proteinase of the clan SC. The clan SC proteinases have a catalytic triad of Ser-Asp-His, and like other Serine proteinases, the active site serine is in a Gly-Xaa-Ser-Xaa -Gly orientation. DPP-8 is a member of a broader family of dipeptidyl peptidases including DPP-2, DPP-4, FAP/Seprase, DPP-6, DPP-9, DPP-10, which have differing substrate specificity and tissue localizations. DPP-8 is most closely related to DPP-9, with which it shares 58% identical residues. DPP-8 has only 27% identity with DPP-4, but seems to have similar substrate specificity. Although DPP-4 and FAP-β form a surface-bound heterodimer in some cells, and homodimers in others, DPP-8 seems to be a cytoplasmic protein. The original publication determined that DPP-8 was a monomer, active at neutral pH, and later publications suggest DPP-8 is a homodimer. Substrates include glucagon-like peptide-1, glucagon-like peptide-2, neuropeptide-Y, but with different efficacy relative to DPP-4. DPP-8 has been found in highest abundance in the testis and placenta, but is found in most tissues. Several different splice variants of DPP-8 were seen in the different tissues, with some tissue-specific expression. Four different human sequences are reported to date; 898, 882, 847 and 782 amino acids in length. The longest sequence of human DPP-8 has a predicted mass of 103.4 kDa, and pI of 5.42. The 882, 847 and 782 amino acid forms are predicted at 101.4, 97.5 and 89.9 kDa respectively, with pIs of 5.26, 5.55 and 5.07. Another three partial sequences that are alternatively spliced have deletions in the catalytic domain, and may not be catalytically active. Over expression of DPP-8 caused decreased viability in cells, but a catalytically dead mutant of DPP-8 had the same results, thus some of the DPP-8 activity may be non-enzymatic. Two of the splice variants have longer aminotermini (16 AA insert), and this may affect localization in the cell. RP1-DPP-8 recognizes all four forms of DPP-8. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Immunogen Synthetic peptide corresponding to the aminoterminal end of human DPP8.
Species Reactivity Human
Specificity This antibody recognizes all four forms of DPP8. This antibody does not recognize other DPP family members.
Tested Applications Western Blot 1:1000
Application Notes Recommended dilutions WB: 1/1000 . Predicted molecular weight: 100 kDa. Use at 1/1000 when using colourimetric substrates, use at 1/5000 when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Raised In Rabbit
Clonality Polyclonal
Isotype IgG
Purity Immunogen Affinity purified
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Formulation Liquid
Concentration 1ug/ul
Storage The undiluted antibody solution is stable for approximately 12 months at -20°C.
Caution to DPP-8 (Dipeptidyl Peptidase-8); Aminterminal end is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

 

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