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Index / Biolin / DNA Kits SensiMix SYBR & Fluorescein Kit / Product Detail : QT615-02 DNA Kits SensiMix SYBR & Fluorescein Kit
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May
29th

#QT615-02 DNA Kits SensiMix SYBR & Fluorescein Kit

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  Price : 372   EUR
422   USD
288   GBP
1563   Zloty
49812   JPY
2871   NOK
3076   SEK
421   CHF

Product name : DNA Kits SensiMix SYBR & Fluorescein Kit

Catalog number : QT615-02

Quantity: 6.25ml

Availability: Yes

Supplier name : Biolin

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More Details about

 

Features and Benefits
 
  • Simple and reproducible: premixed with SYBR® Green I and fluorescein, just add primers and template, reducing errors in set-up
  • Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples
  • Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors
  • 2x master mix: premixed with SYBR® Green I and an internal reference for convenience

 
Instrument Compatibility

Bio-Rad iCycler®, iQTM5 and MyiQ™. (See product selection table). However the kit can is also compatible with several instruments that do not require the use of fluorescein, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.
 
Description

The SensiMix SYBR® & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein signal, such as the Bio-Rad iCycler®, iQ™5 and MyiQ™ platforms. Both SYBR®Green I and fluorescein are premixed for immediate use. 

With SYBR® Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected.

 
Volume

QT615-02: 250 x 50µl Reactions: 5 x 1.25ml
QT615-05: 500 x 50µl Reactions: 10 x 1.25ml
QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml

 
Components

2x Reaction mix consisting of:
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2 (6mM)
  • Fluorescein
  • SYBR® Green I


Plus separate vials of:
  • 50mM MgCl2

 
Kit Size

The kit size is based on a 50µl final reaction volume.
 
Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR® Green I to light.
 
Product Citations
 
  1. Dhawan R. et al. Plant Cell 21: 1000-1019 (2009).
  2. Maatouk D.M. et al. Hum. Mol. Genet. 17(19): 2949-2955 (2008).
  3. Del Rosario B.C. & Pemberton L.F. Mol. Cell. Biol. 28(7): 2113-2124 (2008).
  4. Miller M.R. et al. J. Nutr. 138(11):2179-2185 (2008).

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