GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Biolin / Velocity DNA Polymerase / Product Detail : BIO-21099 Velocity DNA Polymerase
Related keywords:

Jun
27th

#BIO-21099 Velocity DNA Polymerase

Ask technical file .

  Price : 404   EUR
459   USD
313   GBP
1698   Zloty
54106   JPY
3118   NOK
3341   SEK
457   CHF

Product name : Velocity DNA Polymerase

Catalog number : BIO-21099

Quantity: 500 Units

Availability: Yes

Supplier name : Biolin

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Features
 
  • High-speed, high-fidelity DNA polymerase
  • High processivity
  • Fast amplification
  • Shorter PCR runs for longer templates
  • Robust, requires minimal optimization of the reaction

 
Applications
 
  • Cloning techniques where high-fidelity is desirable
  • Blunt-end cloning
  • Amplification of difficult templates
  • Mutagenesis

 
Description

VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high-fidelity with an error-rate of 4.4 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

 
Components
 
  • VELOCITY DNA Polymerase
  • 5x Hi-Fi Buffer (contains 10mM Mg2+)
  • 50mM MgCl2 Solution
  • DMSO

 
Working Concentration

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
 
Concentration

2u/µl
 
Storage Conditions

VELOCITY DNA Polymerase can be stored for 12 months at –20°C.
 
Shipping Conditions

On Dry Ice or Blue Ice.
 
Product Citations
 
  1. Killelea, T. & Connolly, B.A. Chem. BioChem. 12, 1330–1336 (2011).
  2. Thornton, J.K., et al. PLoS ONE 6(8), e23878 (2011).
  3. Paxton, C.W., et al. Am. J. Physiol. 300, C1345-C1355 (2011).
  4. Renaud, C., et al. J. Clin. Virol. 49(1), 21-25 (2010).
  5. Huang, X.X., et al. Am. J. Pathol. 174(4), 1534–1543 (2009).
  6. Norgate, M., et al. PLoS One. 4(11), e7950 (2009).

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Biolin \ Velocity_DNA_Polymerase \ BIO_21099
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
6017 Snell Ave,
Ste 357, San Jose,
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur