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Index / Biolin / BIO_X_ACT Short Mix, 2x / Product Detail : BIO-25025 BIO_X_ACT Short Mix, 2x
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Aug
30th

#BIO-25025 BIO_X_ACT Short Mix, 2x

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  Price : 113   EUR
128   USD
87   GBP
476   Zloty
15172   JPY
874   NOK
937   SEK
128   CHF

Product name : BIO_X_ACT Short Mix, 2x

Catalog number : BIO-25025

Quantity: 100 Reactions

Availability: Yes

Supplier name : Biolin

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More Details about

Features
 
  • Same high performance as BIO-X-ACT™ Short DNA Polymerase
  • Amplifies fragments up to 5Kb
  • Convenient pre-mixed, pre-optimized 2x solutions
  • Reduced risk of contamination
  • Dramatically decreases the time required for reaction setup
  • Reproducible results

 
Applications
 
  • High-fidelity PCR
  • Suitable for cloning

 
Description

BIO-X-ACT™ Short Mix is a complete ready-to-use 2x reaction mix, which enables PCR assays to be performed on problematic templates, with the simple addition of water, template and primers. In order to achieve optimal reaction conditions, the BIO-X-ACT Short Mix contains BIO-X-ACT™ Short DNA Polymerase, MgCl2, ultra-pure dNTPs manufactured by Bioline as well as further additives. The mix has been optimized for a wide variety of templates, and an additional 50mM MgCl2 solution is included should any fine adjustments be required.

BIO-X-ACT Short Mix dramatically reduces the time needed to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured by a reduction in the number of pipetting steps that can lead to pipetting errors.

 
Volume

BIO-25025: 100 x 50µl Reactions: 2 x 1.25ml
BIO-25026: 500 x 50µl Reactions: 10 x 1.25ml

 
Extended Stability

A sample of BIO-X-ACT Short Mix was stored at +20°C over a five-week period and tested daily. No detectable loss of activity was evidenced. However, in view of the possibility of microbial contamination, please adhere to the recommended storage conditions.
 
Concentration

2x
 
Storage Conditions

BIO-X-ACT Short Mix can be stored for up to 12 months at -20°C, or up to 2 weeks at +4°C.
 
Product Citations
 
  1. Eastman, R. T., et al. Antimicrob. Agents Chemother. 55, 3908-3916 (2011).
  2. Salame, T. M., et al. Micro. Biotech. 3(1), 93–106 (2010).
  3. Sharbati, S., et al. BMC Microbiol. 9, 31 (2009).
  4. Maruvada, R., et al. FASEB J. 23, 3967-77 (2009).
  5. Ondzigjhi, C.A., et al. Plant Cell 20(8), 2205–20 (2008).
  6. Eastham, R. T., et al. Mol. Biochem. Parasitol. 152(1), 66-71 (2007).
  7. Caffaro, C. E., et al. PNAS 103(44), 16176-16181 (2006).

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