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Index / Biolin / Hydroxymethylated dCTP, 100mM / Product Detail : BIO-39046 Hydroxymethylated dCTP, 100mM
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May
24th

#BIO-39046 Hydroxymethylated dCTP, 100mM

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  Price : 369   EUR
418   USD
286   GBP
1550   Zloty
49383   JPY
2846   NOK
3049   SEK
417   CHF

Product name : Hydroxymethylated dCTP, 100mM

Catalog number : BIO-39046

Quantity: 25µmol, (1 x 250µl)

Availability: Yes

Supplier name : Biolin

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More Details about

 
 
Features
 
  • Ultra high purity >99% triphosphate by HPLC
  • Readily incorporated by standard DNA polymerases
  • Hydroxymethylated substrates can be ligated by standard ligases
  • DNase, RNase and Nickase free

 
Applications
 
  • Epigenetic/Methylation studies
  • Studies of hydroxymethylated DNA/protein interaction
  • Site-Directed Mutagenesis
  • Substitution of dCTP in a wide variety of molecular biology assays
  • Structural and activity studies of the restriction/modification systems of different organisms
  • Labeling of DNA in vitro

 
Description

5-hmC (5-Hydroxymethylcytosine) is a modified nucleotide, a hydroxylated and methylated form of cytosine originally found in T-even bacteriophages and subsequently mammalian genomes. Described as the "sixth base of the genome", this new tool opens up multiple avenues of investigation for DNA methylation and epigenomics research.

Manufactured in a purpose-built facility, Bioline has developed a novel enzymatic synthesis method for producing highly purified Hydroxymethyl dCTP that mimics the biological steps in the synthesis of Hydroxymethyl dCTP from T-even phages. Highly purified Hydroxymethyl dCTP can be used in a number of molecular biological applications.

Hydroxymethyl dCTP can be used as a substrate for several DNA polymerases under conditions that permit the amplification of DNA containing hydroxymethylated cytosine in place of cytosine. Hydroxymethyl dCTPs can be used to discriminate between the different DNA molecules synthesized in one or several PCR cycles. By the use of appropriate enzymes, it is possible to separate the unhydroxymethylated starting material from the hemi-hydroxymethylated intermediate (produced by a single primer extension reaction) and from the fully-hydroxymethylated end product.

This ability to generate PCR products in which cytosine is uniformly replaced by hydroxymethylated cytosine can be applied to: epigenetic/methylation studies, studies of hydroxymethylated DNA/protein interaction, and forensic DNA analysis. Further applications include the development of novel strategies for site-directed mutagenesis and the production of DNA fragments resistant to cleavage by a wide range of restriction endonucleases and can be useful in the generation of cDNA libraries.

Bioline's ultra-pure hydroxymethyl dCTP has been cited in a key epigenetics paper by Tahiliani et al. published in Science from the Department of Pathology, Harvard Medical School and Immune Disease Institute.

For more information regarding Bulk, Custom and OEM dNTP services please visit our dedicated microsite.

 
Storage Conditions

Hydroxymethyl dCTP can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.
 
Shipping Conditions

On Dry Ice or Blue Ice.
 
Product Citations
 
  1. Guo, J.U., et al. Cell 145(3), 423-434 (2011).
  2. Stroud, H., et al., Genome Biol. 12(6), R54 (2011).
  3. Kinney, S.M., et al. J. Biol. Chem. 286, 24685-24693 (2011).
  4. Wang, H., et al. NAR doi: 10.1093/nar/gkr607 (2011).
  5. Robertson, A.B., et al. NAR 39, e55 (2011).
  6. Szwagierczak, A., et al. NAR 39(12), 5149-5156 (2011).
  7. Huang, Y., et al. PLoS ONE 5(1), e8888 (2010).
  8. Jin, S-G., et al. NAR 38, e125 (2010).
  9. Szwagierczak, A., et al. NAR 38, e181 (2010).
  10. Nestor, C., et al. Biotechniques 48, 317-319 (2010.
  11. Tahiliani, M., et al. Science 324, 930-935 (2009).

 
Physical constants, spectral and HPLC analysis

Product HMdCTP Lithium 100mM Solution
Nomenclature 5 hydroxymethyl 2´-deoxycytidine-5´-triphosphate
Formula C11H14N3O14P3Li4
Molecular Weight 520.918g/mol
λmax pH 7.0 275nm
ε at λmax 7.7 E x mmol-1 x cm-1
A250/A260 0.90±0.03
A250/A260 1.33±0.03
Concentration 100mM±2%
Appearance Clear Colorless Solution
pH of Solution 7.5
dNTP (HPLC Area) >99%
dNDP (HPLC Area) <1%
DNases, RNases, Nicking Activity Negative
Storage at –20°C
Stability >24 months

 
HPLC Profile of >99% pure dNTPs

HMdCTP
Hydroxymethyl dCTP

 

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