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Index / Biolin / SensiMix II Probe Kit / Product Detail : BIO-91002 SensiMix II Probe Kit
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Jun
26th

#BIO-91002 SensiMix II Probe Kit

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  Price : 353   EUR
400   USD
273   GBP
1483   Zloty
47236   JPY
2722   NOK
2917   SEK
399   CHF

Product name : SensiMix II Probe Kit

Catalog number : BIO-91002

Quantity: 250 rxn

Availability: Yes

Supplier name : Biolin

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More Details about

 

eatures and Benefits
 
  • Superior PCR Efficiency: with sequence specific probes
  • High specificity: reproducibility and reduced background
  • Simple and reproducible: Ideal for multiplexing and gene expression analysis
  • Flexible: Compatibility with all real-time PCR instruments

 
Instrument Compatibility

Roche LightCycler® 480 platform, Bio-Rad Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).
 
Description

The SensiMix™ II Probe Kit is a high-performance reagent designed for superior sensitivity and specificity on all real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpion®, Assay On Demand®, allelic discrimination and molecular beacon probes, delivering superior performance in gene expression analysis (Fig. 1) and multiplexing (Fig. 2). The SensiMix II Probe Kit employs a hot-start DNA polymerase, for high PCR specificity and sensitivity. Since SensiMix possesses no polymerase activity during reaction set-up, the kit greatly reduces non-specific amplification including primer-dimer formation. After pre-heating, SensiMix becomes fully activated and in conjunction with specially optimized buffer chemistry, generates reliable and highly reproducible data on all real-time PCR instruments. 

For ease-of-use and added convenience, SensiMix II Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs and stabilizers. 

 
Volume

BIO-91002: 250 x 50µl Reactions: 5 x 1.25ml
BIO-91005: 500 x 50µl Reactions: 10 x 1.25ml
BIO-91020: 2000 x 50µl Reactions: 40 x 1.25ml

 
Components

2x Reaction mix consisting of:
  • Heat-activated DNA polymerase
  • Ultra-pure dNTPs
  • MgCl2 (6mM)

Plus separate vials of:
  • 50mM MgCl2
  • 25µM ROX dye

 
Kit Size

The kit size is based on a 50µl final reaction volume.
 
Storage Conditions

6 months at -20°C. Avoid exposure of the ROX™ to light.

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