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#BIO-21098 DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase

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Product name : DNA Polymerases - For High-Fidelity Applications : VELOCITY DNA Polymerase

Catalog number : BIO-21098

Quantity: 250 Units

Availability: Yes

Supplier name : Biolin

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More Details about

Features

 

• High-speed, high-fidelity DNA polymerase
• High processivity
• Fast amplification
• Shorter PCR runs for longer templates
• Robust, requires minimal optimization of the reaction

 

Applications

 

• Cloning techniques where high-fidelity is desirable
• Blunt-end cloning
• Amplification of difficult templates
• Mutagenesis

 

Description

VELOCITY is a fast thermostable enzyme possessing 5´-3´ DNA polymerase and 3´-5´ proofreading exonuclease activities. VELOCITY provides high-fidelity with an error-rate of 4.4 x 10^-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (4.4 x 10^-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).
 

Working Concentration

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.
 

Concentration

2u/µl

 

 

Storage Conditions

VELOCITY DNA Polymerase can be stored for 12 months at –20°C.
 

Shipping Conditions

On Dry Ice or Blue Ice.
 

Product Citations

 

1. Killelea, T. & Connolly, B.A. Chem. BioChem. 12, 1330–1336 (2011).
2. Thornton, J.K., et al. PLoS ONE 6(8), e23878 (2011).
3. Paxton, C.W., et al. Am. J. Physiol. 300, C1345-C1355 (2011).
4. Renaud, C., et al. J. Clin. Virol. 49(1), 21-25 (2010).
5. Huang, X.X., et al. Am. J. Pathol. 174(4), 1534–1543 (2009).
6. Norgate, M., et al. PLoS One. 4(11), e7950 (2009).

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