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Index / Biolin / DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase / Product Detail : BIO-21115 DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase
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Feb
23th
#BIO-21115 DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase
Price : | 487 | EUR |
553 | USD | |
378 | GBP | |
2049 | Zloty | |
65271 | JPY | |
3762 | NOK | |
4031 | SEK | |
551 | CHF |
Product name : DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase
Catalog number : BIO-21115
Quantity: 1000 units
Availability: Yes
Supplier name : Biolin
Data sheet: Ask more or other datasheet now !
More Details about
"The MyTaq HS sample was so much better than anything else we have used, we have ordered more already."
Features
New generation of antibody-based hot-start polymerase
The highest specificity and the best performance
New buffer system including dNTPs and MgCl2
Fast PCR reactions
Red dye for loading the gel directly
Applications
High-throughput PCR
Assays with prolonged reaction setup on the bench or liquid handling
Amplification of challenging targets susceptible to mispriming
Colony PCR
Multiplexing
High Specificity of amplification of difficult templates ( such as GC rich)
Genotyping
TA cloning
Two-Step RT-PCR
Description
MyTaq™ HS Red DNA Polymerase is a high performance PCR reagent that is powered by antibody-mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. MyTaq HS Red does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification including primer-dimer formation. The advanced formulation of MyTaq HS Red allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield (Fig. 1). This new enzyme from Bioline is supplied with 5x MyTaq buffer system, a proprietary formulation that saves time and delivers superior results, as it contains dNTPs, MgCl2 and enhancers at optimal concentrations which removes the need for optimization.
The specially designed MyTaq Red formulation does not interfere with the PCR reaction and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer.
MyTaq HS Red only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.
Components
•MyTaq HS DNA Polymerase
•5x MyTaq Reaction Buffer
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
Concentration
5u/µl
Storage Conditions
MyTaq HS Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
2500 Units Vilas HERE
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Related products : DNA Polymerases - For Hot-Start Applications : MyTaq HS Red DNA Polymerase
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Pathways :
WP2185: Purine metabolism
WP1438: Influenza A virus infection
WP1644: DNA replication
WP1663: Homologous recombination
WP1672: Mismatch repair
WP1693: Purine metabolism
WP1694: Pyrimidine metabolism
WP1699: RNA polymerase
WP1905: RNA Polymerase I, RNA Polymerase III, and Mitochondrial Transcription
WP1906: RNA Polymerase II Transcription
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