Tissue-Tek O.C.T. compound is a formulation of transparent, water-soluble glycols and resins that provides a solid matrix to encapsulate tissue samples and adhere to the specimen holder for consistent sectioning at cryostat operating temperatures of -10°C and below. Tissue-Tek O.C.T. is a water-soluble embedding compound for cryosectioning. It is mainly composed of glycols and synthetic resins. Tissue-Tek O.C.T. is used as a matrix for tissue cryosection How to use an OCT compound?
What is Tissue-Tek?
Put 2 drops of OCT in a plastic cryo-mould. Place the tissue on top in the correct orientation for cutting. Gently pour the OCT over the tissue, taking care to avoid bubbles, until the tissue is no longer exposed. Place the mould over the aluminium plate on dry ice for rapid freezing..
This formulation of glycols and water-soluble resins at optimum shear temperature (O.C.T.) from Tissue-Tek provides a convenient preparation matrix for cryostat sections at temperatures of -10°C (14°F) and below.
The most popular and consistent inclusion medium for cryotomy.
This compound is used to rapidly embed fresh tissue samples for cold cutting with a cryostat. Tissue-Tek O.C.T. compound is a formulation of transparent, water-soluble glycols and resins that form a solid matrix to encapsulate tissue samples and adhere to the sample holder for coherent sections at cryostat operating temperatures of -10°C and below.
Unwanted background staining is eliminated as no residue is left on the slides during staining.
O.C.T. stands for optimum cutting temperature. The high viscosity of Tissue-Tek's O.C.T. compound allows rapid freezing for optimal cutting quality.
O.C.T. compound is sold in 118 ml (4 oz) bottles; 12/pack.
Sakura's Tissue-Tek O.C.T. Compound is a formulation of water-soluble glycols and resins that provides a convenient preparation matrix for cryostat slides at temperatures as low as -10°C and below. No residue is left on slides during the staining procedure, eliminating unwanted background staining.
Long-term storage of tissue embedded in O.C.T. Compound at -80°C for more than 10 years!
ATTENTION: To prevent the tissue from drying out, it must be completely covered.
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