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Index / ABM / Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody / Product Detail : Y051444 Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody
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Oct
23th

#Y051444 Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody

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Product name : Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody

Catalog number : Y051444

Quantity: 250ug

Availability: Yes

Supplier name : ABM

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About this Product :

Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody.
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Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody storing antibdies for longer storage periods of the Anti-Calpain-5 H-TRA-3; Carboxyterminal end of Domain IV. antibody we recommend - 21C or lower and for short periods lees than 1 month we suggest +5C fridge temperatures.
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More Details about

Antibody Type Primary Antibody
Description Anti-Calpain-5 (H-TRA-3); Carboxyterminal end of Domain IV. is a This antibody is a Rabbit antibody to the cysteine protease, Calpain-5, made with a synthetic peptide based on the carboxyterminal end of the large subunit, using the human sequence. The antibody binds to Calpain-5, but does not cross react with the other calpain family members (calpain-1, calpain-2, Calpain-3, ncl-2, ncl-3, etc.). This antibody reacts to Domain-IV of Calpain-5, and detects both latent and aminoprocessed (activated) Calpain-5. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant
Immunogen Synthetic peptide of Human Calpain 5 based on the carboxyterminal end of the large subunit.
Species Reactivity Human, Mouse, Rat
Specificity The antibody binds to reduced and non-reduced Calpain 5, but does not cross react with the other calpain family members (calpain 1, calpain 2, Calpain 3, ncl 2, ncl 3, etc.). It reacts to Domain-IV of Calpain 5, and detects both latent and aminoprocessed (activated) Calpain 5.
Tested Applications Western Blot 1:1000
Application Notes Calpain-5, also known as human TRA-3 orthologue, is an intracellular, papain-like cysteine protease. Unlike the ubiquitous Calpain-1 and Calpain-2, Calpain-5 is expressed in a more limited range of tissues, including testis, colon, kidney, liver and trachea. In C. elegans, H-TRA-3 is involved in sex determination, but the mammalian role is currently unknown. The calpain family members consist of a common small subunit (Calpain-4), and a large variable subunit (although it is not clear if all calpains contain a small subunit). Calpain-5 has not yet been reported to associate with a small subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV (domain T in calpains 5 & 6). Calpain-5 does not have the classical EF hand calcium-binding domain-IV, and it is not clear if calcium enhances calpain-5. RP2Calpain-5 recognizes latent and active Calpain-5; the latent large subunit is 73 KD, and the amino terminal truncation at activation yields an approximately 58 KD form. A cascade of smaller forms can be seen with further activation. RP2Calpain-5 Anti-Calpain-5 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 73 KD, 58 KD, and a series of further cleaved active forms. RP2Calpain-5 will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. Calpain-5 is a cellular protein, and cell lysate works well for Western blots. Rat, mouse and human Calpain-5 are detected with RP2Calpain-5. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Raised In Rabbit
Clonality Polyclonal
Isotype IgG
Purity Immunogen Affinity purified
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Formulation Liquid
Concentration 1ug/ul
Storage The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20°C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 4°C with 0.05% Sodium Azide as preservative.
Caution Anti-Calpain-5 (H-TRA-3); Carboxyterminal end of Domain IV. is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

 

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