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Index / ABM / Anti-ADAM-13 A Disintegrin And Metalloproteinase-13 X-ADAM-13; Propeptide domain antibody / Product Detail : Y051501 Anti-ADAM-13 A Disintegrin And Metalloproteinase-13 X-ADAM-13; Propeptide domain antibody
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#Y051501 Anti-ADAM-13 A Disintegrin And Metalloproteinase-13 X-ADAM-13; Propeptide domain antibody

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  Price : 1208   EUR
1371   USD
937   GBP
5073   Zloty
161605   JPY
9314   NOK
9980   SEK
1366   CHF

Product name : Anti-ADAM-13 A Disintegrin And Metalloproteinase-13 X-ADAM-13; Propeptide domain antibody

Catalog number : Y051501

Quantity: 250ug

Availability: Yes

Supplier name : ABM

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More Details about

Antibody Type Primary Antibody
Description Anti-ADAM-13 (A Disintegrin And Metalloproteinase-13, X-ADAM-13); Propeptide domain is a The antibody is a polyclonal antibody made to the metalloproteinase ADAM-13. The antibody is made to a synthetic peptide based on the amino end of XenopusADAM-13. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant. ADAM-13 was first described as a protein expressed in somatic mesoderm and neural crest cells, in developing Xenopus embryos. ADAM13 was found in liver, heart and intestines from adult Xenopus. Other papers investigated SH3 ligand domains in the Cytoplasmic portion of ADAM13, demonstrating regulation routes for ADAM13 via Src and Src tyrosine kinase. ADAM13 may also act as a cell-attachment molecule, by binding integrins through the cysteine-rich domain. A member of the metalloproteinase family containing disintegrin-like domains (ADAMs) the functions of ADAM-13 are still poorly understood. ADAM-13 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, as well as disintegrin, cysteine-rich, EFG-like, transmembrane and Cytoplasmic domains. ADAM-13 has been shown to be proteolytically active, cleaving fibronectin after binding it to the EGF-like domain. ADAM-13 is also shed from cells in culture, cleaved aminoterminal from the transmembrane domain, and is released into the culture media. Shed ADAM-13 is a 52 kD protein, and can form complexes with a2-macroglobulin, suggesting it is a competent protease. Xenopus ADAM-13 has greatest homology with human ADAM-33 (51% identical), and is 46% identical with human or mouse ADAM-12 or ADAM-19. It is still unclear if any of these ADAMs are species orthologs of Xenopus ADAM13, but there are significant differences between the related sequences, suggesting that ADAM-13 may be a unique protein. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length Xenopus ADAM13 sequence codes for a 914 amino acid protein. Predicted mass is 99.749 kD, but glycosylation and cyteine-rich regions give Xenopus ADAM-13 an apparent MW of 120 kD unprocessed, and 97 kD processed forms, on reduced SDS PAGE gels. ADAM-13 contains a putative furin cleavage site, suggesting that a prohormone convertase cleaves the propeptide domain away from the catalytic domain. RP1ADAM-13 recognizes bands of 120 kD (minor band), 92 kD (major band), and cleaved products_recover at 68 kD, 40 kD in reduced Western blots of cell lysates. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Immunogen Synthetic peptide based on the amino end of Xenopus ADAM13.
Species Reactivity Xl
Specificity recognises metalloproteinase ADAM-13
Tested Applications Western Blot 1:1000
Application Notes Recommended dilutions WB: Recommended starting dilution of 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes bands of 120 kDa (minor band), 92 kDa (major band), and cleaved products_recover at 68 kDa, 40 kDa in reduced Western blots of cell lysates. (Predicted molecular weight: 100 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Raised In Rabbit
Clonality Polyclonal
Isotype IgG
Purity Immunogen Affinity purified
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Formulation Liquid
Concentration 1ug/ul
Storage The undiluted antibody solution is stable for approximately 6 months at 4°C. For longer storage, store at -20C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at -80°C. Avoid unnecessary freeze-thaw cycles.
Caution Anti-ADAM-13 (A Disintegrin And Metalloproteinase-13, X-ADAM-13); Propeptide domain is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

 

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