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Index / MarkerGene / Green FAM Caspase 9 Assay Kit _ 100tests / Product Detail : M0828 Green FAM Caspase 9 Assay Kit _ 100tests
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#M0828 Green FAM Caspase 9 Assay Kit _ 100tests

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  Price : 793   EUR
901   USD
615   GBP
3334   Zloty
106209   JPY
6121   NOK
6559   SEK
897   CHF

Product name : Green FAM Caspase 9 Assay Kit _ 100tests

Catalog number : M0828

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Availability: Yes

Supplier name : MarkerGene

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More Details about

Description:
FAM FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases intracellularly.

Application:
The methodology is based on the unique cell-permeable and non-cytotoxic reagents called the Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. For the FAM FLICA™ kits, which fluoresce green, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of the specific caspase is used (EX:488nm / EM:520nm), compatible with most fluorescence microscopes or microplate readers.
STAINING PROTOCOL Staining apoptotic cells with the FLICA™ kit can be completed within a few minutes. However, the FLICA™ kit is used with living cells, which require periodic maintenance and cultivation several days in advance. In addition, once the proper number of cells has been cultivated, time must be allotted for the induction process. The FLICA™ kit works with your current apoptosis protocols - induce apoptosis as you normally would and then label the cells with FLICA™. 1. Culture cells to a density optimal for apoptosis induction according to your specific induction protocol, but not to exceed 106 cells/mL. 2. At the same time, culture a non-induced negative control cell population at the same density as the induced population for every labeling condition. 3. Induce apoptosis following your protocol (such as treating Jurkat cells with 2 mg/ml camptothecin for 3 hours). 4. Dilute the 10X wash buffer to 1X. 5. Reconstitute the vial of lyophilized FLICA™ with DMSO to form the 150X FLICA™ stock concentrate. 6. Dilute the 150X FLICA™ stock to the 30X working solution. 7. Stain cells by adding the 30X FLICA™ solution. 8. Incubate for 1 hour. 9. Wash and spin cells. 10. If desired, label cells with Hoechst 33342 stain. 11. If desired, fix cells. 12. Analyze data via microtiter plate fluorometry or fluorescence microscopy.

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