GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc 6017 Snell Ave, Ste 357, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / MarkerGene / Red Sulforhodamine Poly Caspases Assay Kit _ 100tests / Product Detail : M0834 Red Sulforhodamine Poly Caspases Assay Kit _ 100tests
Related keywords:

Jul
31th

#M0834 Red Sulforhodamine Poly Caspases Assay Kit _ 100tests

Ask technical file .

  Price : 839   EUR
953   USD
651   GBP
3527   Zloty
112364   JPY
6476   NOK
6939   SEK
949   CHF

Product name : Red Sulforhodamine Poly Caspases Assay Kit _ 100tests

Catalog number : M0834

Quantity:

Availability: Yes

Supplier name : MarkerGene

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

More Details about

Description:
SR-FLICA™ Apoptosis Detection Kits use a novel approach to detect active caspases.

Application:
The methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA™). Once inside the cell, the FLICA™ inhibitor binds covalently to the active caspase. The SR FLICA™ reagent excites at 550 nm and has a maximum emission range of 590-600 nm. For the SR FLICA™ kits, which fluoresce red, a sulforhodamine-labeled fluoromethyl ketone peptide inhibitor of caspases is used. The red SR-FLICA™ kits contain a fluorescent-labeled inhibitor, either SR-VAD-FMK (sulforhodaminyl-L-valylalanylaspartyl fluoromethyl ketone), or SR-DEVD-FMK (sulforhodaminyl-L-aspartylglutamylvalyl-aspartylfluoro-methyl ketone). VAD is an amino acid sequence targeted by all caspases, while DEVD is targeted by caspases-3 and -7. When added to a population of cells, the FLICA™ probe enters each cell and covalently binds to a reactive cysteine residue that resides on the large subunit of the caspase heterodimer, thereby inhibiting further enzymatic activity. Because the FLICA™ reagent is covalently coupled to the enzyme, it is retained in the cell, while any unbound FLICA™ reagent will diffuse out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the number of active caspase enzymes that were present in the cell when the reagent was added. Cells that contain the bound FLICA™ can be analyzed by 96-well-plate based fluorometry, and fluorescence microscopy. Because the FLICA™ reagent SR-VAD-FMK irreversibly binds to many activated caspases (caspases-1, -3, -4, -5, -6, -7, -8 and -9), it can be used as a generic probe for the detection of most caspases. In comparison, when the FLICA™ reagent SR-DEVD-FMK enters the cell, it primarily binds to caspase-3, therefore it can be used to measure the amount of active caspase-3 that was present in the cell at the time when the SR-DEVD-FMK FLICA™ reagent was added. Cells labeled with the FLICA™ reagent may be read immediately or preserved for 24 hours using the fixative. Unfixed SR samples may be analyzed with Hoechst stain.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
MarkerGene \ Red_Sulforhodamine_Poly_Caspases_Assay_Kit___100tests \ M0834
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur





GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017

BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG

france@gentaur.com | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur